Prokaryotic expression of HPV 16L1 and optimization of expression conditions

To construct the HPV16 L1 prokaryotic expression plasmid and to optimize its expression. A pair of primers was designed according to plasmid sequences of pGEX-KG and the HPV16L1 genes published by GeneBank. The DNA fragment of 1500 bp was amplified by PCR from the HPV recombinant plasmid with HPV16L...

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Veröffentlicht in:	Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban 2010-07, Vol.39 (4), p.395-398
Hauptverfasser:	Peng, Fang-yi, Jiang, Hai-rong, Chen, Yuan-xiang, Chen, Sheng-zhen, Lin, Zhi-hua, Peng, Fang-liang, Zhao, Wei-bin, Chen, Bao-de
Format:	Artikel
Sprache:	chi
Schlagworte:	Cancer Vaccines - biosynthesis Capsid Proteins - biosynthesis Capsid Proteins - genetics Cloning, Molecular Escherichia coli - genetics Escherichia coli - metabolism Genetic Vectors Human papillomavirus 16 - genetics Oncogene Proteins, Viral - biosynthesis Oncogene Proteins, Viral - genetics Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics
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