Effects of sex and estrogen on chicken ductus arteriosus reactivity
Sex hormones have an important influence on cardiovascular physiology and pathophysiology and sex differences in vascular reactivity have been widely demonstrated. In the present study we hypothesized 1) the presence of sexual dimorphism in chicken ductus arteriosus (DA) responsiveness to contractil...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2010-05, Vol.298 (5), p.R1217-R1224 |
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| creator | Flinsenberg, Thijs W H van der Sterren, Saskia van Cleef, Anne N H Schuurman, Marijn J Agren, Pia Villamor, Eduardo |
| description | Sex hormones have an important influence on cardiovascular physiology and pathophysiology and sex differences in vascular reactivity have been widely demonstrated. In the present study we hypothesized 1) the presence of sexual dimorphism in chicken ductus arteriosus (DA) responsiveness to contractile and relaxant stimuli and 2) that estrogens are vasoactive in the chicken DA. In vitro contractions (assessed with a wire myograph) induced by normoxia, KCl, 4-aminopyridine, norepinephrine, phenylephrine, U46619, or endothelin-1, as well as relaxations induced by ACh, sodium nitroprusside, BAY 41-2272, PGE(2), isoproterenol, forskolin,Y-27632, and hydroxyfasudil were not significantly different between males and females. The estrogen 17beta-estradiol elicited concentration-dependent relaxation of KCl-, phenylephrine-, and oxygen-induced active tone in male and female chicken DA. The stereoisomer 17alpha-estradiol showed lesser relaxant effects, and the selective estrogen receptor (ER) agonists 4,4',4''-(4-propyl-[(1)H]pyrazole-1,3,5-triyl)tris-phenol (ERalpha) and 2,3-bis(4-hydroxyphenyl)-propionitrile (ERbeta) did not show any effect. There were no sex differences in the responses to estrogen. Endothelium removal or the presence of the soluble guanylate cyclase inhibitor ODQ, the K(+) channel blockers tetraethylammonium, glibenclamide, and charybdotoxin, or the ER antagonist fulvestrant did not modify 17beta-estradiol-induced relaxation. CaCl(2) (30 muM-10 mM) induced concentration-dependent contraction in DA rings depolarized by 62.5 mM KCl or stimulated with 21% O(2) in Ca(2+)-free medium. Preincubation with 17beta-estradiol or the L-type Ca(2+) channel blocker nifedipine produced an inhibition of CaCl(2)-induced contractions. In conclusion, there are no sex-related differences in chicken DA reactivity. The estrogen 17beta-estradiol induces an endothelium-independent relaxation of chicken DA that is not mediated by ER activation. This relaxant effect is, at least partially, due to inhibition of Ca(2+) entry from extracellular space. |
| doi_str_mv | 10.1152/ajpregu.00839.2009 |
| format | Article |
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In the present study we hypothesized 1) the presence of sexual dimorphism in chicken ductus arteriosus (DA) responsiveness to contractile and relaxant stimuli and 2) that estrogens are vasoactive in the chicken DA. In vitro contractions (assessed with a wire myograph) induced by normoxia, KCl, 4-aminopyridine, norepinephrine, phenylephrine, U46619, or endothelin-1, as well as relaxations induced by ACh, sodium nitroprusside, BAY 41-2272, PGE(2), isoproterenol, forskolin,Y-27632, and hydroxyfasudil were not significantly different between males and females. The estrogen 17beta-estradiol elicited concentration-dependent relaxation of KCl-, phenylephrine-, and oxygen-induced active tone in male and female chicken DA. The stereoisomer 17alpha-estradiol showed lesser relaxant effects, and the selective estrogen receptor (ER) agonists 4,4',4''-(4-propyl-[(1)H]pyrazole-1,3,5-triyl)tris-phenol (ERalpha) and 2,3-bis(4-hydroxyphenyl)-propionitrile (ERbeta) did not show any effect. There were no sex differences in the responses to estrogen. Endothelium removal or the presence of the soluble guanylate cyclase inhibitor ODQ, the K(+) channel blockers tetraethylammonium, glibenclamide, and charybdotoxin, or the ER antagonist fulvestrant did not modify 17beta-estradiol-induced relaxation. CaCl(2) (30 muM-10 mM) induced concentration-dependent contraction in DA rings depolarized by 62.5 mM KCl or stimulated with 21% O(2) in Ca(2+)-free medium. Preincubation with 17beta-estradiol or the L-type Ca(2+) channel blocker nifedipine produced an inhibition of CaCl(2)-induced contractions. In conclusion, there are no sex-related differences in chicken DA reactivity. The estrogen 17beta-estradiol induces an endothelium-independent relaxation of chicken DA that is not mediated by ER activation. This relaxant effect is, at least partially, due to inhibition of Ca(2+) entry from extracellular space.</description><identifier>ISSN: 0363-6119</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.00839.2009</identifier><identifier>PMID: 20164203</identifier><identifier>CODEN: AJPRDO</identifier><language>eng</language><publisher>United States: American Physiological Society</publisher><subject>15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology ; 4-Aminopyridine - pharmacology ; Acetylcholine ; Anatomy & physiology ; Animals ; Calcium - metabolism ; Cardiovascular system ; Chick Embryo ; Chickens ; Colforsin - pharmacology ; Dinoprostone - pharmacology ; Ductus Arteriosus - drug effects ; Ductus Arteriosus - embryology ; Ductus Arteriosus - physiology ; Estradiol - metabolism ; Estradiol - pharmacology ; Estrogens ; Female ; Hormones ; Male ; Nitroprusside - pharmacology ; Norepinephrine - pharmacology ; Oxygen - metabolism ; Oxygen - pharmacology ; Potassium Channel Blockers - pharmacology ; Potassium Chloride - pharmacology ; Poultry ; Pyrazoles - pharmacology ; Pyridines - pharmacology ; Receptors, Estrogen - agonists ; Sex Characteristics ; Signal Transduction - drug effects ; Signal Transduction - physiology ; Vasoconstriction - drug effects ; Vasoconstriction - physiology ; Vasoconstrictor Agents - pharmacology ; Vasodilation - drug effects ; Vasodilation - physiology</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 2010-05, Vol.298 (5), p.R1217-R1224</ispartof><rights>Copyright American Physiological Society May 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-3da4c54ea497938d24aa01f3ee7ef12e571d50a43ec40ad2fed46116f46213643</citedby><cites>FETCH-LOGICAL-c329t-3da4c54ea497938d24aa01f3ee7ef12e571d50a43ec40ad2fed46116f46213643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20164203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flinsenberg, Thijs W H</creatorcontrib><creatorcontrib>van der Sterren, Saskia</creatorcontrib><creatorcontrib>van Cleef, Anne N H</creatorcontrib><creatorcontrib>Schuurman, Marijn J</creatorcontrib><creatorcontrib>Agren, Pia</creatorcontrib><creatorcontrib>Villamor, Eduardo</creatorcontrib><title>Effects of sex and estrogen on chicken ductus arteriosus reactivity</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><description>Sex hormones have an important influence on cardiovascular physiology and pathophysiology and sex differences in vascular reactivity have been widely demonstrated. In the present study we hypothesized 1) the presence of sexual dimorphism in chicken ductus arteriosus (DA) responsiveness to contractile and relaxant stimuli and 2) that estrogens are vasoactive in the chicken DA. In vitro contractions (assessed with a wire myograph) induced by normoxia, KCl, 4-aminopyridine, norepinephrine, phenylephrine, U46619, or endothelin-1, as well as relaxations induced by ACh, sodium nitroprusside, BAY 41-2272, PGE(2), isoproterenol, forskolin,Y-27632, and hydroxyfasudil were not significantly different between males and females. The estrogen 17beta-estradiol elicited concentration-dependent relaxation of KCl-, phenylephrine-, and oxygen-induced active tone in male and female chicken DA. The stereoisomer 17alpha-estradiol showed lesser relaxant effects, and the selective estrogen receptor (ER) agonists 4,4',4''-(4-propyl-[(1)H]pyrazole-1,3,5-triyl)tris-phenol (ERalpha) and 2,3-bis(4-hydroxyphenyl)-propionitrile (ERbeta) did not show any effect. There were no sex differences in the responses to estrogen. Endothelium removal or the presence of the soluble guanylate cyclase inhibitor ODQ, the K(+) channel blockers tetraethylammonium, glibenclamide, and charybdotoxin, or the ER antagonist fulvestrant did not modify 17beta-estradiol-induced relaxation. CaCl(2) (30 muM-10 mM) induced concentration-dependent contraction in DA rings depolarized by 62.5 mM KCl or stimulated with 21% O(2) in Ca(2+)-free medium. Preincubation with 17beta-estradiol or the L-type Ca(2+) channel blocker nifedipine produced an inhibition of CaCl(2)-induced contractions. In conclusion, there are no sex-related differences in chicken DA reactivity. The estrogen 17beta-estradiol induces an endothelium-independent relaxation of chicken DA that is not mediated by ER activation. This relaxant effect is, at least partially, due to inhibition of Ca(2+) entry from extracellular space.</description><subject>15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology</subject><subject>4-Aminopyridine - pharmacology</subject><subject>Acetylcholine</subject><subject>Anatomy & physiology</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Cardiovascular system</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Colforsin - pharmacology</subject><subject>Dinoprostone - pharmacology</subject><subject>Ductus Arteriosus - drug effects</subject><subject>Ductus Arteriosus - embryology</subject><subject>Ductus Arteriosus - physiology</subject><subject>Estradiol - metabolism</subject><subject>Estradiol - pharmacology</subject><subject>Estrogens</subject><subject>Female</subject><subject>Hormones</subject><subject>Male</subject><subject>Nitroprusside - pharmacology</subject><subject>Norepinephrine - pharmacology</subject><subject>Oxygen - metabolism</subject><subject>Oxygen - pharmacology</subject><subject>Potassium Channel Blockers - pharmacology</subject><subject>Potassium Chloride - pharmacology</subject><subject>Poultry</subject><subject>Pyrazoles - pharmacology</subject><subject>Pyridines - pharmacology</subject><subject>Receptors, Estrogen - agonists</subject><subject>Sex Characteristics</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - physiology</subject><subject>Vasoconstriction - drug effects</subject><subject>Vasoconstriction - physiology</subject><subject>Vasoconstrictor Agents - pharmacology</subject><subject>Vasodilation - drug effects</subject><subject>Vasodilation - physiology</subject><issn>0363-6119</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkDtPAzEQhC0EghD4AxToREN1Yf26i0sUhYcUiQZqy9jrcCE5B_sOkX-PQwIF1Y60M6PRR8gFhRGlkt2YxTrivB8BjLkaMQB1QAb5wUoqFBySAfCKlxWl6oScprQAAMEFPyYnDGglGPABmUy9R9ulIvgi4VdhWldg6mKYY1uEtrBvjX3P0vW261NhYoexCSnLiMZ2zWfTbc7IkTfLhOf7OyQvd9PnyUM5e7p_nNzOSsuZ6krujLBSoBGqVnzsmDAGqOeINXrKUNbUSTCCoxVgHPPoRB5feVExyivBh-R617uO4aPPK_WqSRaXS9Ni6JOuxVhJBZxl59U_5yL0sc3jNNtyklzKbGI7k40hpYher2OzMnGjKegtYL0HrH8A620why73zf3rCt1f5Jco_wa4j3eI</recordid><startdate>201005</startdate><enddate>201005</enddate><creator>Flinsenberg, Thijs W H</creator><creator>van der Sterren, Saskia</creator><creator>van Cleef, Anne N H</creator><creator>Schuurman, Marijn J</creator><creator>Agren, Pia</creator><creator>Villamor, Eduardo</creator><general>American Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201005</creationdate><title>Effects of sex and estrogen on chicken ductus arteriosus reactivity</title><author>Flinsenberg, Thijs W H ; 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Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flinsenberg, Thijs W H</au><au>van der Sterren, Saskia</au><au>van Cleef, Anne N H</au><au>Schuurman, Marijn J</au><au>Agren, Pia</au><au>Villamor, Eduardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of sex and estrogen on chicken ductus arteriosus reactivity</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><date>2010-05</date><risdate>2010</risdate><volume>298</volume><issue>5</issue><spage>R1217</spage><epage>R1224</epage><pages>R1217-R1224</pages><issn>0363-6119</issn><eissn>1522-1490</eissn><coden>AJPRDO</coden><abstract>Sex hormones have an important influence on cardiovascular physiology and pathophysiology and sex differences in vascular reactivity have been widely demonstrated. In the present study we hypothesized 1) the presence of sexual dimorphism in chicken ductus arteriosus (DA) responsiveness to contractile and relaxant stimuli and 2) that estrogens are vasoactive in the chicken DA. In vitro contractions (assessed with a wire myograph) induced by normoxia, KCl, 4-aminopyridine, norepinephrine, phenylephrine, U46619, or endothelin-1, as well as relaxations induced by ACh, sodium nitroprusside, BAY 41-2272, PGE(2), isoproterenol, forskolin,Y-27632, and hydroxyfasudil were not significantly different between males and females. The estrogen 17beta-estradiol elicited concentration-dependent relaxation of KCl-, phenylephrine-, and oxygen-induced active tone in male and female chicken DA. The stereoisomer 17alpha-estradiol showed lesser relaxant effects, and the selective estrogen receptor (ER) agonists 4,4',4''-(4-propyl-[(1)H]pyrazole-1,3,5-triyl)tris-phenol (ERalpha) and 2,3-bis(4-hydroxyphenyl)-propionitrile (ERbeta) did not show any effect. There were no sex differences in the responses to estrogen. Endothelium removal or the presence of the soluble guanylate cyclase inhibitor ODQ, the K(+) channel blockers tetraethylammonium, glibenclamide, and charybdotoxin, or the ER antagonist fulvestrant did not modify 17beta-estradiol-induced relaxation. CaCl(2) (30 muM-10 mM) induced concentration-dependent contraction in DA rings depolarized by 62.5 mM KCl or stimulated with 21% O(2) in Ca(2+)-free medium. Preincubation with 17beta-estradiol or the L-type Ca(2+) channel blocker nifedipine produced an inhibition of CaCl(2)-induced contractions. In conclusion, there are no sex-related differences in chicken DA reactivity. The estrogen 17beta-estradiol induces an endothelium-independent relaxation of chicken DA that is not mediated by ER activation. This relaxant effect is, at least partially, due to inhibition of Ca(2+) entry from extracellular space.</abstract><cop>United States</cop><pub>American Physiological Society</pub><pmid>20164203</pmid><doi>10.1152/ajpregu.00839.2009</doi></addata></record> |
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| subjects | 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology 4-Aminopyridine - pharmacology Acetylcholine Anatomy & physiology Animals Calcium - metabolism Cardiovascular system Chick Embryo Chickens Colforsin - pharmacology Dinoprostone - pharmacology Ductus Arteriosus - drug effects Ductus Arteriosus - embryology Ductus Arteriosus - physiology Estradiol - metabolism Estradiol - pharmacology Estrogens Female Hormones Male Nitroprusside - pharmacology Norepinephrine - pharmacology Oxygen - metabolism Oxygen - pharmacology Potassium Channel Blockers - pharmacology Potassium Chloride - pharmacology Poultry Pyrazoles - pharmacology Pyridines - pharmacology Receptors, Estrogen - agonists Sex Characteristics Signal Transduction - drug effects Signal Transduction - physiology Vasoconstriction - drug effects Vasoconstriction - physiology Vasoconstrictor Agents - pharmacology Vasodilation - drug effects Vasodilation - physiology |
| title | Effects of sex and estrogen on chicken ductus arteriosus reactivity |
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