Detection of Structural Dynamics by FRET: A Photon Distribution and Fluorescence Lifetime Analysis of Systems with Multiple States

Two complementary methods in confocal single-molecule fluorescence spectroscopy are presented to analyze conformational dynamics by Förster resonance energy transfer (FRET) measurements considering simulated and experimental data. First, an extension of photon distribution analysis (PDA) is applied...

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Veröffentlicht in:	The journal of physical chemistry. B 2010-06, Vol.114 (23), p.7983-7995
Hauptverfasser:	Kalinin, Stanislav, Valeri, Alessandro, Antonik, Matthew, Felekyan, Suren, Seidel, Claus A. M
Format:	Artikel
Sprache:	eng
Schlagworte:	B: Biophysical Chemistry Fluorescence Resonance Energy Transfer Models, Theoretical Photons Spectrometry, Fluorescence
Online-Zugang:	Volltext
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