Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family
Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of...
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| Veröffentlicht in: | Glycobiology (Oxford) 2010-09, Vol.20 (9), p.1077-1088 |
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| container_title | Glycobiology (Oxford) |
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| creator | Zhang, Lei Lau, Kam Cheng, Jiansong Yu, Hai Li, Yanhong Sugiarto, Go Huang, Shengshu Ding, Li Thon, Vireak Wang, Peng G Chen, Xi |
| description | Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family. |
| doi_str_mv | 10.1093/glycob/cwq068 |
| format | Article |
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The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family.</description><identifier>EISSN: 1460-2423</identifier><identifier>DOI: 10.1093/glycob/cwq068</identifier><identifier>PMID: 20466652</identifier><language>eng</language><publisher>England</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - genetics ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - metabolism ; Base Sequence ; Cloning, Molecular ; Fucosyltransferases - genetics ; Fucosyltransferases - isolation & purification ; Fucosyltransferases - metabolism ; Glycosyltransferases - genetics ; Glycosyltransferases - metabolism ; Helicobacter hepaticus - genetics ; Models, Biological ; Molecular Sequence Data ; Multigene Family ; Phylogeny ; Recombinant Proteins - genetics ; Recombinant Proteins - isolation & purification ; Recombinant Proteins - metabolism ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid</subject><ispartof>Glycobiology (Oxford), 2010-09, Vol.20 (9), p.1077-1088</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20466652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Lau, Kam</creatorcontrib><creatorcontrib>Cheng, Jiansong</creatorcontrib><creatorcontrib>Yu, Hai</creatorcontrib><creatorcontrib>Li, Yanhong</creatorcontrib><creatorcontrib>Sugiarto, Go</creatorcontrib><creatorcontrib>Huang, Shengshu</creatorcontrib><creatorcontrib>Ding, Li</creatorcontrib><creatorcontrib>Thon, Vireak</creatorcontrib><creatorcontrib>Wang, Peng G</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><title>Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family</title><title>Glycobiology (Oxford)</title><addtitle>Glycobiology</addtitle><description>Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Fucosyltransferases - genetics</subject><subject>Fucosyltransferases - isolation & purification</subject><subject>Fucosyltransferases - metabolism</subject><subject>Glycosyltransferases - genetics</subject><subject>Glycosyltransferases - metabolism</subject><subject>Helicobacter hepaticus - genetics</subject><subject>Models, Biological</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Phylogeny</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><issn>1460-2423</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kD1PwzAYhC0kREthZEXemEL9HWesKmiRKrF0Yokc53Ua5NhpnIDy76kETCednueGQ-iBkmdKCr5u_GxjtbbfZ6L0FVpSoUjGBOMLdJvSJyFUUS1v0IIRoZSSbIn8Hnx7kYwdYcAn6M3Y2inh_YmQnOEGAmAINtaQsMEhfoHHxvcnQzOeucnGNPtxMCE5GEwCXIGPoWlDg8eIt5uPGe-OlGJnutbPd-jaGZ_g_i9X6Pj6ctzus8P77m27OWS9lCyrC64EZRpUrmtZCyuU5JpW0jpaCw2scJxKYp1WlblUkijLZC6sdaZyWvAVevqd7Yd4niCNZdcmC96bAHFKZS50ISlV-YV8_COnqoO67Ie2M8Nc_v_DfwD7ImYI</recordid><startdate>201009</startdate><enddate>201009</enddate><creator>Zhang, Lei</creator><creator>Lau, Kam</creator><creator>Cheng, Jiansong</creator><creator>Yu, Hai</creator><creator>Li, Yanhong</creator><creator>Sugiarto, Go</creator><creator>Huang, Shengshu</creator><creator>Ding, Li</creator><creator>Thon, Vireak</creator><creator>Wang, Peng G</creator><creator>Chen, Xi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201009</creationdate><title>Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family</title><author>Zhang, Lei ; Lau, Kam ; Cheng, Jiansong ; Yu, Hai ; Li, Yanhong ; Sugiarto, Go ; Huang, Shengshu ; Ding, Li ; Thon, Vireak ; Wang, Peng G ; Chen, Xi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p552-d9364128e678d5d4c465381b5cf1d48e29f3150cf86baf1d506c2574ccfabf843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacterial Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Fucosyltransferases - genetics</topic><topic>Fucosyltransferases - isolation & purification</topic><topic>Fucosyltransferases - metabolism</topic><topic>Glycosyltransferases - genetics</topic><topic>Glycosyltransferases - metabolism</topic><topic>Helicobacter hepaticus - genetics</topic><topic>Models, Biological</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Phylogeny</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Lau, Kam</creatorcontrib><creatorcontrib>Cheng, Jiansong</creatorcontrib><creatorcontrib>Yu, Hai</creatorcontrib><creatorcontrib>Li, Yanhong</creatorcontrib><creatorcontrib>Sugiarto, Go</creatorcontrib><creatorcontrib>Huang, Shengshu</creatorcontrib><creatorcontrib>Ding, Li</creatorcontrib><creatorcontrib>Thon, Vireak</creatorcontrib><creatorcontrib>Wang, Peng G</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Glycobiology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Lei</au><au>Lau, Kam</au><au>Cheng, Jiansong</au><au>Yu, Hai</au><au>Li, Yanhong</au><au>Sugiarto, Go</au><au>Huang, Shengshu</au><au>Ding, Li</au><au>Thon, Vireak</au><au>Wang, Peng G</au><au>Chen, Xi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family</atitle><jtitle>Glycobiology (Oxford)</jtitle><addtitle>Glycobiology</addtitle><date>2010-09</date><risdate>2010</risdate><volume>20</volume><issue>9</issue><spage>1077</spage><epage>1088</epage><pages>1077-1088</pages><eissn>1460-2423</eissn><abstract>Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family.</abstract><cop>England</cop><pmid>20466652</pmid><doi>10.1093/glycob/cwq068</doi><tpages>12</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Base Sequence Cloning, Molecular Fucosyltransferases - genetics Fucosyltransferases - isolation & purification Fucosyltransferases - metabolism Glycosyltransferases - genetics Glycosyltransferases - metabolism Helicobacter hepaticus - genetics Models, Biological Molecular Sequence Data Multigene Family Phylogeny Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid |
| title | Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family |
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