Why do p-nitro-substituted aryl azides provide unintended dark reactions with proteins?

Aryl azide-mediated photo cross-linking has been widely used to obtain structural features in biological systems, even though the reactive species generated upon photolysis in aqueous solution have not been well characterized. We have established a mechanistic framework for the formation of adducts...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of photochemistry and photobiology. B, Biology Biology, 2010-07, Vol.100 (1), p.19-29
Hauptverfasser:	Popova, Tatyana V., Reinbolt, Joseph, Ehresmann, Bernard, Shakirov, Makhmut M., Serebriakova, Marina V., Gerassimova, Yulia V., Knorre, Dmitri G., Godovikova, Tatyana S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels - chemistry Amino Acid Sequence Aromatic hydroxylamine Aromatic nitroso compound Aryl azide Azides - chemistry Biotin - chemistry Cross-Linking Reagents - chemistry Long-lived intermediate Modified tryptophan Nitroso Compounds - chemistry Photoaffinity labeling Photolysis Streptavidin - chemistry Streptavidin - metabolism Tryptophan - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!