Influence of the Use of Laser Phototherapy (λ660 or 790 nm) on the Survival of Cutaneous Flaps on Diabetic Rats
Objective: The aim of this study was to assess and compare the effects of laser phototherapy (LPT) on cutaneous flaps on diabetic rats. Background: Diabetes mellitus is characterized by high blood glucose levels. Its main complications are delayed wound healing, an impaired blood supply, and a decre...
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| Veröffentlicht in: | Photomedicine and laser surgery 2010-08, Vol.28 (4), p.483-488 |
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| creator | Santos, Nicole R.S. dos Santos, Jean N. dos Reis, João A. Oliveira, Priscila C. de Sousa, Ana Paula C. de Carvalho, Carolina M. Soares, Luiz G.P. Marques, Aparecida M.C. Pinheiro, Antonio Luiz B. |
| description | Objective:
The aim of this study was to assess and compare the effects of laser phototherapy (LPT) on cutaneous flaps on diabetic rats.
Background:
Diabetes mellitus is characterized by high blood glucose levels. Its main complications are delayed wound healing, an impaired blood supply, and a decrease in collagen production. Cutaneous flaps are routinely used in several surgical procedures, and most failures are related to poor blood supply. LPT has been studied using several healing models.
Animals and Methods:
Twelve Wistar rats were randomized into three groups: group 1 (G1; diabetic animals without treatment), group 2 (G2; diabetic animals irradiated with λ680 nm), and group 3 (G3; diabetic animals irradiated with λ790 nm). Diabetes was induced with streptozotocin. A 2- x 8-cm cutaneous flap was raised on the dorsum of each animal, and a plastic sheet was introduced between the flap and the bed to cause poor blood supply. Nonirradiated animals acted as controls. The dose per session was 40 J/cm
2
. Laser light was applied transcutaneously and fractioned on 16 contact points at the wound margins (16 × 2.5 J/cm
2
). Animal death occurred on day 8 after surgery. Specimens were taken, processed, cut, stained with eosin
(
HE) and sirius red, and underwent histological analysis.
Results:
It is shown that accute inflammation was mostly discrete for G3. Chronic inflammation was more evident for G2. Fibroblast number was higher for G3. Angiogenesis was more evident for G3. Necrosis was more evident for G2. Statistical analysis among all groups showed significant differences (
p
= 0.04) on the level of acute inflammation between G1 and G3, tissue necrosis between G1 and G2 (
p
= 0.03), chronic inflammation between (
p
= 0.04), fibroblastic proliferation between G2 and G3 (
p
= 0.05), and neovascularization between G2 and G3 (
p
= 0.04).
Conclusion:
LPT was effective in increasing angiogenesis as seen on irradiated subjects and was more pronounced when IR laser light was used. |
| doi_str_mv | 10.1089/pho.2009.2500 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_748946875</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A235631466</galeid><sourcerecordid>A235631466</sourcerecordid><originalsourceid>FETCH-LOGICAL-c442t-eace0a9bbcc804a53516d8756ae5486da741cf0444d2098c51e791a3477e86d83</originalsourceid><addsrcrecordid>eNqFkc-K1TAYxYsozji6dCsBFzqLXpM2f5fD1XEGLijqrEOafvVG2qQm7cDs3PpMvoMPMU9iOr0gwoBkkY-T3zl85BTFc4I3BEv1ZtyHTYWx2lQM4wfFMWFMlJIx_HCZqSoZJfKoeJLSN4wrJVT9uDgiStaEKnFcxEvf9TN4Cyh0aNoDukp3484kiOjjPkwhq9GMN-j171-cYxQiEgrf_vjph1MU_J3p8xyv3bXpF-d2noyHMCd03psxLchbZxqYnEWfzJSeFo860yd4drhPiqvzd1-2F-Xuw_vL7dmutJRWUwnGAjaqaayVmBpWM8JbKRg3wKjkrRGU2A5TStsKK2kZAaGIqakQkJ9lfVK8WnPHGL7PkCY9uGSh79fttKBSUZ4TM_lyJb-aHrTzXZiisQutz6qa8fxXnGdqcw-VTwuDs8FD57L-j6FcDTaGlCJ0eoxuMPFGE6yX8nQuTy_l6aW8zL84LDw3A7R_6UNbGahXYJGN972DBuL0n9g_ID6k4w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>748946875</pqid></control><display><type>article</type><title>Influence of the Use of Laser Phototherapy (λ660 or 790 nm) on the Survival of Cutaneous Flaps on Diabetic Rats</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Santos, Nicole R.S. ; dos Santos, Jean N. ; dos Reis, João A. ; Oliveira, Priscila C. ; de Sousa, Ana Paula C. ; de Carvalho, Carolina M. ; Soares, Luiz G.P. ; Marques, Aparecida M.C. ; Pinheiro, Antonio Luiz B.</creator><creatorcontrib>Santos, Nicole R.S. ; dos Santos, Jean N. ; dos Reis, João A. ; Oliveira, Priscila C. ; de Sousa, Ana Paula C. ; de Carvalho, Carolina M. ; Soares, Luiz G.P. ; Marques, Aparecida M.C. ; Pinheiro, Antonio Luiz B.</creatorcontrib><description>Objective:
The aim of this study was to assess and compare the effects of laser phototherapy (LPT) on cutaneous flaps on diabetic rats.
Background:
Diabetes mellitus is characterized by high blood glucose levels. Its main complications are delayed wound healing, an impaired blood supply, and a decrease in collagen production. Cutaneous flaps are routinely used in several surgical procedures, and most failures are related to poor blood supply. LPT has been studied using several healing models.
Animals and Methods:
Twelve Wistar rats were randomized into three groups: group 1 (G1; diabetic animals without treatment), group 2 (G2; diabetic animals irradiated with λ680 nm), and group 3 (G3; diabetic animals irradiated with λ790 nm). Diabetes was induced with streptozotocin. A 2- x 8-cm cutaneous flap was raised on the dorsum of each animal, and a plastic sheet was introduced between the flap and the bed to cause poor blood supply. Nonirradiated animals acted as controls. The dose per session was 40 J/cm
2
. Laser light was applied transcutaneously and fractioned on 16 contact points at the wound margins (16 × 2.5 J/cm
2
). Animal death occurred on day 8 after surgery. Specimens were taken, processed, cut, stained with eosin
(
HE) and sirius red, and underwent histological analysis.
Results:
It is shown that accute inflammation was mostly discrete for G3. Chronic inflammation was more evident for G2. Fibroblast number was higher for G3. Angiogenesis was more evident for G3. Necrosis was more evident for G2. Statistical analysis among all groups showed significant differences (
p
= 0.04) on the level of acute inflammation between G1 and G3, tissue necrosis between G1 and G2 (
p
= 0.03), chronic inflammation between (
p
= 0.04), fibroblastic proliferation between G2 and G3 (
p
= 0.05), and neovascularization between G2 and G3 (
p
= 0.04).
Conclusion:
LPT was effective in increasing angiogenesis as seen on irradiated subjects and was more pronounced when IR laser light was used.</description><identifier>ISSN: 1549-5418</identifier><identifier>EISSN: 1557-8550</identifier><identifier>DOI: 10.1089/pho.2009.2500</identifier><identifier>PMID: 19831497</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Blood sugar ; Care and treatment ; Diabetes ; Diabetes Mellitus, Experimental ; Graft Survival - radiation effects ; Lasers in medicine ; Low-Level Light Therapy ; Male ; Measurement ; Methods ; Neovascularization ; Observations ; Original Articles ; Phototherapy ; Random Allocation ; Rats ; Rats, Wistar ; Surgical Flaps</subject><ispartof>Photomedicine and laser surgery, 2010-08, Vol.28 (4), p.483-488</ispartof><rights>2010, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2010 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-eace0a9bbcc804a53516d8756ae5486da741cf0444d2098c51e791a3477e86d83</citedby><cites>FETCH-LOGICAL-c442t-eace0a9bbcc804a53516d8756ae5486da741cf0444d2098c51e791a3477e86d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19831497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santos, Nicole R.S.</creatorcontrib><creatorcontrib>dos Santos, Jean N.</creatorcontrib><creatorcontrib>dos Reis, João A.</creatorcontrib><creatorcontrib>Oliveira, Priscila C.</creatorcontrib><creatorcontrib>de Sousa, Ana Paula C.</creatorcontrib><creatorcontrib>de Carvalho, Carolina M.</creatorcontrib><creatorcontrib>Soares, Luiz G.P.</creatorcontrib><creatorcontrib>Marques, Aparecida M.C.</creatorcontrib><creatorcontrib>Pinheiro, Antonio Luiz B.</creatorcontrib><title>Influence of the Use of Laser Phototherapy (λ660 or 790 nm) on the Survival of Cutaneous Flaps on Diabetic Rats</title><title>Photomedicine and laser surgery</title><addtitle>Photomed Laser Surg</addtitle><description>Objective:
The aim of this study was to assess and compare the effects of laser phototherapy (LPT) on cutaneous flaps on diabetic rats.
Background:
Diabetes mellitus is characterized by high blood glucose levels. Its main complications are delayed wound healing, an impaired blood supply, and a decrease in collagen production. Cutaneous flaps are routinely used in several surgical procedures, and most failures are related to poor blood supply. LPT has been studied using several healing models.
Animals and Methods:
Twelve Wistar rats were randomized into three groups: group 1 (G1; diabetic animals without treatment), group 2 (G2; diabetic animals irradiated with λ680 nm), and group 3 (G3; diabetic animals irradiated with λ790 nm). Diabetes was induced with streptozotocin. A 2- x 8-cm cutaneous flap was raised on the dorsum of each animal, and a plastic sheet was introduced between the flap and the bed to cause poor blood supply. Nonirradiated animals acted as controls. The dose per session was 40 J/cm
2
. Laser light was applied transcutaneously and fractioned on 16 contact points at the wound margins (16 × 2.5 J/cm
2
). Animal death occurred on day 8 after surgery. Specimens were taken, processed, cut, stained with eosin
(
HE) and sirius red, and underwent histological analysis.
Results:
It is shown that accute inflammation was mostly discrete for G3. Chronic inflammation was more evident for G2. Fibroblast number was higher for G3. Angiogenesis was more evident for G3. Necrosis was more evident for G2. Statistical analysis among all groups showed significant differences (
p
= 0.04) on the level of acute inflammation between G1 and G3, tissue necrosis between G1 and G2 (
p
= 0.03), chronic inflammation between (
p
= 0.04), fibroblastic proliferation between G2 and G3 (
p
= 0.05), and neovascularization between G2 and G3 (
p
= 0.04).
Conclusion:
LPT was effective in increasing angiogenesis as seen on irradiated subjects and was more pronounced when IR laser light was used.</description><subject>Animals</subject><subject>Blood sugar</subject><subject>Care and treatment</subject><subject>Diabetes</subject><subject>Diabetes Mellitus, Experimental</subject><subject>Graft Survival - radiation effects</subject><subject>Lasers in medicine</subject><subject>Low-Level Light Therapy</subject><subject>Male</subject><subject>Measurement</subject><subject>Methods</subject><subject>Neovascularization</subject><subject>Observations</subject><subject>Original Articles</subject><subject>Phototherapy</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Surgical Flaps</subject><issn>1549-5418</issn><issn>1557-8550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-K1TAYxYsozji6dCsBFzqLXpM2f5fD1XEGLijqrEOafvVG2qQm7cDs3PpMvoMPMU9iOr0gwoBkkY-T3zl85BTFc4I3BEv1ZtyHTYWx2lQM4wfFMWFMlJIx_HCZqSoZJfKoeJLSN4wrJVT9uDgiStaEKnFcxEvf9TN4Cyh0aNoDukp3484kiOjjPkwhq9GMN-j171-cYxQiEgrf_vjph1MU_J3p8xyv3bXpF-d2noyHMCd03psxLchbZxqYnEWfzJSeFo860yd4drhPiqvzd1-2F-Xuw_vL7dmutJRWUwnGAjaqaayVmBpWM8JbKRg3wKjkrRGU2A5TStsKK2kZAaGIqakQkJ9lfVK8WnPHGL7PkCY9uGSh79fttKBSUZ4TM_lyJb-aHrTzXZiisQutz6qa8fxXnGdqcw-VTwuDs8FD57L-j6FcDTaGlCJ0eoxuMPFGE6yX8nQuTy_l6aW8zL84LDw3A7R_6UNbGahXYJGN972DBuL0n9g_ID6k4w</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Santos, Nicole R.S.</creator><creator>dos Santos, Jean N.</creator><creator>dos Reis, João A.</creator><creator>Oliveira, Priscila C.</creator><creator>de Sousa, Ana Paula C.</creator><creator>de Carvalho, Carolina M.</creator><creator>Soares, Luiz G.P.</creator><creator>Marques, Aparecida M.C.</creator><creator>Pinheiro, Antonio Luiz B.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100801</creationdate><title>Influence of the Use of Laser Phototherapy (λ660 or 790 nm) on the Survival of Cutaneous Flaps on Diabetic Rats</title><author>Santos, Nicole R.S. ; dos Santos, Jean N. ; dos Reis, João A. ; Oliveira, Priscila C. ; de Sousa, Ana Paula C. ; de Carvalho, Carolina M. ; Soares, Luiz G.P. ; Marques, Aparecida M.C. ; Pinheiro, Antonio Luiz B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-eace0a9bbcc804a53516d8756ae5486da741cf0444d2098c51e791a3477e86d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Blood sugar</topic><topic>Care and treatment</topic><topic>Diabetes</topic><topic>Diabetes Mellitus, Experimental</topic><topic>Graft Survival - radiation effects</topic><topic>Lasers in medicine</topic><topic>Low-Level Light Therapy</topic><topic>Male</topic><topic>Measurement</topic><topic>Methods</topic><topic>Neovascularization</topic><topic>Observations</topic><topic>Original Articles</topic><topic>Phototherapy</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Surgical Flaps</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santos, Nicole R.S.</creatorcontrib><creatorcontrib>dos Santos, Jean N.</creatorcontrib><creatorcontrib>dos Reis, João A.</creatorcontrib><creatorcontrib>Oliveira, Priscila C.</creatorcontrib><creatorcontrib>de Sousa, Ana Paula C.</creatorcontrib><creatorcontrib>de Carvalho, Carolina M.</creatorcontrib><creatorcontrib>Soares, Luiz G.P.</creatorcontrib><creatorcontrib>Marques, Aparecida M.C.</creatorcontrib><creatorcontrib>Pinheiro, Antonio Luiz B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photomedicine and laser surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santos, Nicole R.S.</au><au>dos Santos, Jean N.</au><au>dos Reis, João A.</au><au>Oliveira, Priscila C.</au><au>de Sousa, Ana Paula C.</au><au>de Carvalho, Carolina M.</au><au>Soares, Luiz G.P.</au><au>Marques, Aparecida M.C.</au><au>Pinheiro, Antonio Luiz B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of the Use of Laser Phototherapy (λ660 or 790 nm) on the Survival of Cutaneous Flaps on Diabetic Rats</atitle><jtitle>Photomedicine and laser surgery</jtitle><addtitle>Photomed Laser Surg</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>28</volume><issue>4</issue><spage>483</spage><epage>488</epage><pages>483-488</pages><issn>1549-5418</issn><eissn>1557-8550</eissn><abstract>Objective:
The aim of this study was to assess and compare the effects of laser phototherapy (LPT) on cutaneous flaps on diabetic rats.
Background:
Diabetes mellitus is characterized by high blood glucose levels. Its main complications are delayed wound healing, an impaired blood supply, and a decrease in collagen production. Cutaneous flaps are routinely used in several surgical procedures, and most failures are related to poor blood supply. LPT has been studied using several healing models.
Animals and Methods:
Twelve Wistar rats were randomized into three groups: group 1 (G1; diabetic animals without treatment), group 2 (G2; diabetic animals irradiated with λ680 nm), and group 3 (G3; diabetic animals irradiated with λ790 nm). Diabetes was induced with streptozotocin. A 2- x 8-cm cutaneous flap was raised on the dorsum of each animal, and a plastic sheet was introduced between the flap and the bed to cause poor blood supply. Nonirradiated animals acted as controls. The dose per session was 40 J/cm
2
. Laser light was applied transcutaneously and fractioned on 16 contact points at the wound margins (16 × 2.5 J/cm
2
). Animal death occurred on day 8 after surgery. Specimens were taken, processed, cut, stained with eosin
(
HE) and sirius red, and underwent histological analysis.
Results:
It is shown that accute inflammation was mostly discrete for G3. Chronic inflammation was more evident for G2. Fibroblast number was higher for G3. Angiogenesis was more evident for G3. Necrosis was more evident for G2. Statistical analysis among all groups showed significant differences (
p
= 0.04) on the level of acute inflammation between G1 and G3, tissue necrosis between G1 and G2 (
p
= 0.03), chronic inflammation between (
p
= 0.04), fibroblastic proliferation between G2 and G3 (
p
= 0.05), and neovascularization between G2 and G3 (
p
= 0.04).
Conclusion:
LPT was effective in increasing angiogenesis as seen on irradiated subjects and was more pronounced when IR laser light was used.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>19831497</pmid><doi>10.1089/pho.2009.2500</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Photomedicine and laser surgery, 2010-08, Vol.28 (4), p.483-488 |
| issn | 1549-5418 1557-8550 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Blood sugar Care and treatment Diabetes Diabetes Mellitus, Experimental Graft Survival - radiation effects Lasers in medicine Low-Level Light Therapy Male Measurement Methods Neovascularization Observations Original Articles Phototherapy Random Allocation Rats Rats, Wistar Surgical Flaps |
| title | Influence of the Use of Laser Phototherapy (λ660 or 790 nm) on the Survival of Cutaneous Flaps on Diabetic Rats |
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