Connexin 43 hemichannel regulates H9c2 cell proliferation by modulating intracellular ATP and [Ca^2＋]
Connexin 43 （Cx43）, known to be the main protein building blocks of gap junctions and hemichannels in mammalian heart, plays an important role in cardiocytes proliferation. Gap junctional intercellular communication has been suggested to be necessary for cellular proliferation and differentiation. H...
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| Veröffentlicht in: | Acta biochimica et biophysica Sinica 2010-07, Vol.42 (7), p.472-482 |
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| creator | Song, Dongli Liu, Xiaoyu Liu, Rujiao Yang, Ling Zuo, Ji Liu, Wen |
| description | Connexin 43 （Cx43）, known to be the main protein building blocks of gap junctions and hemichannels in mammalian heart, plays an important role in cardiocytes proliferation. Gap junctional intercellular communication has been suggested to be necessary for cellular proliferation and differentiation. However, the effect of Cx43 hemichannel on cardiocytes proliferation and the mechanism remain unclear. In this study, rat heart cell line H9c2 was used. The Cx43 location, the proliferation rate and hemichannel activity of H9c2 cells and Wnt-3a^＋-H9c2 cells were investigated and the changes of intraceilular ATP and [Ca^2＋] were determined. Results showed that the inhibited hemichannel induced by 18β-glycyrrhetinic acid （GA） evoked intracellular ATP and [Ca^2＋] increase and enhanced H9c2 cell proliferation. Wnt-3a＋-H9c2 cells displayed enhanced hemichannel activity and proliferation rate. Inhibited hemichannel of Wnt-3a＋-H9c2 cells induced by 18β-GA decreased intracellular ATP, increased [Ca^2＋], and enhanced the proliferation of H9c2 cells. This study validated the role of hemichannel in H9c2 cell proliferation regulation, and showed a mechanism involved in the regulation of H9c2 cell proliferation. The proliferation could be enhanced by Cx43 hemichannel-mediated ATP release accompanying intraceilular [Ca^2＋]change. However, different changes of ATP were observed in Wnt-3a＋-H9c2 cells. These findings provided new insights into the molecular mechanisms of proliferation regulation in H9c2 cells and the effect of Wnt-3a on intraceliular ATP. |
| doi_str_mv | 10.1093/abbs/gmq047 |
| format | Article |
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Gap junctional intercellular communication has been suggested to be necessary for cellular proliferation and differentiation. However, the effect of Cx43 hemichannel on cardiocytes proliferation and the mechanism remain unclear. In this study, rat heart cell line H9c2 was used. The Cx43 location, the proliferation rate and hemichannel activity of H9c2 cells and Wnt-3a^＋-H9c2 cells were investigated and the changes of intraceilular ATP and [Ca^2＋] were determined. Results showed that the inhibited hemichannel induced by 18β-glycyrrhetinic acid （GA） evoked intracellular ATP and [Ca^2＋] increase and enhanced H9c2 cell proliferation. Wnt-3a＋-H9c2 cells displayed enhanced hemichannel activity and proliferation rate. Inhibited hemichannel of Wnt-3a＋-H9c2 cells induced by 18β-GA decreased intracellular ATP, increased [Ca^2＋], and enhanced the proliferation of H9c2 cells. This study validated the role of hemichannel in H9c2 cell proliferation regulation, and showed a mechanism involved in the regulation of H9c2 cell proliferation. The proliferation could be enhanced by Cx43 hemichannel-mediated ATP release accompanying intraceilular [Ca^2＋]change. However, different changes of ATP were observed in Wnt-3a＋-H9c2 cells. These findings provided new insights into the molecular mechanisms of proliferation regulation in H9c2 cells and the effect of Wnt-3a on intraceliular ATP.</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1093/abbs/gmq047</identifier><identifier>PMID: 20705586</identifier><language>eng</language><publisher>China: Oxford University Press</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; ATP ; Blotting, Western ; Calcium - metabolism ; Cell Line ; Cell Proliferation ; Cell Survival - drug effects ; Connexin 43 - genetics ; Connexin 43 - metabolism ; Connexin 43 - physiology ; Cx43 ; Dose-Response Relationship, Drug ; Fluorescence Recovery After Photobleaching ; Glycyrrhetinic Acid - analogs & derivatives ; Glycyrrhetinic Acid - pharmacology ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - drug effects ; Myocytes, Cardiac - metabolism ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; Wnt Proteins - genetics ; Wnt Proteins - metabolism ; Wnt3 Protein ; 心肌细胞 ; 细胞内 ; 细胞增殖 ; 细胞活性 ; 细胞间隙连接通讯 ; 连接蛋白</subject><ispartof>Acta biochimica et biophysica Sinica, 2010-07, Vol.42 (7), p.472-482</ispartof><rights>The Author 2010. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-fe163adbf4e4ff70112e6f2b97dbe0d73646c25f1d99d8612543ccbdd835bb0c3</citedby><cites>FETCH-LOGICAL-c313t-fe163adbf4e4ff70112e6f2b97dbe0d73646c25f1d99d8612543ccbdd835bb0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90160X/90160X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20705586$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Dongli</creatorcontrib><creatorcontrib>Liu, Xiaoyu</creatorcontrib><creatorcontrib>Liu, Rujiao</creatorcontrib><creatorcontrib>Yang, Ling</creatorcontrib><creatorcontrib>Zuo, Ji</creatorcontrib><creatorcontrib>Liu, Wen</creatorcontrib><title>Connexin 43 hemichannel regulates H9c2 cell proliferation by modulating intracellular ATP and [Ca^2＋]</title><title>Acta biochimica et biophysica Sinica</title><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><description>Connexin 43 （Cx43）, known to be the main protein building blocks of gap junctions and hemichannels in mammalian heart, plays an important role in cardiocytes proliferation. Gap junctional intercellular communication has been suggested to be necessary for cellular proliferation and differentiation. However, the effect of Cx43 hemichannel on cardiocytes proliferation and the mechanism remain unclear. In this study, rat heart cell line H9c2 was used. The Cx43 location, the proliferation rate and hemichannel activity of H9c2 cells and Wnt-3a^＋-H9c2 cells were investigated and the changes of intraceilular ATP and [Ca^2＋] were determined. Results showed that the inhibited hemichannel induced by 18β-glycyrrhetinic acid （GA） evoked intracellular ATP and [Ca^2＋] increase and enhanced H9c2 cell proliferation. Wnt-3a＋-H9c2 cells displayed enhanced hemichannel activity and proliferation rate. Inhibited hemichannel of Wnt-3a＋-H9c2 cells induced by 18β-GA decreased intracellular ATP, increased [Ca^2＋], and enhanced the proliferation of H9c2 cells. This study validated the role of hemichannel in H9c2 cell proliferation regulation, and showed a mechanism involved in the regulation of H9c2 cell proliferation. The proliferation could be enhanced by Cx43 hemichannel-mediated ATP release accompanying intraceilular [Ca^2＋]change. However, different changes of ATP were observed in Wnt-3a＋-H9c2 cells. These findings provided new insights into the molecular mechanisms of proliferation regulation in H9c2 cells and the effect of Wnt-3a on intraceliular ATP.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>ATP</subject><subject>Blotting, Western</subject><subject>Calcium - metabolism</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Cell Survival - drug effects</subject><subject>Connexin 43 - genetics</subject><subject>Connexin 43 - metabolism</subject><subject>Connexin 43 - physiology</subject><subject>Cx43</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fluorescence Recovery After Photobleaching</subject><subject>Glycyrrhetinic Acid - analogs & derivatives</subject><subject>Glycyrrhetinic Acid - pharmacology</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - drug effects</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Wnt Proteins - genetics</subject><subject>Wnt Proteins - metabolism</subject><subject>Wnt3 Protein</subject><subject>心肌细胞</subject><subject>细胞内</subject><subject>细胞增殖</subject><subject>细胞活性</subject><subject>细胞间隙连接通讯</subject><subject>连接蛋白</subject><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtO3DAUhi1UxK1dsUdWF-0CBXyLPVmiUcsgjQQLuqpay9eMIXFm7ESCZ-CVeCdegUQzdNvVOfr16T9HHwCnGF1gVNFLpXW-rNsNYmIPHGHBykIQgT6NOxekqDArD8Fxzg8IUc4xOgCHBAlUljN-BOp5F6N7ChEyCleuDWalxqCBydVDo3qX4aIyBBrXNHCduiZ4l1Qfugj1M2w7O0Eh1jDEPqmJGoMEr-7voIoW_p6rv-Tt9eXPZ7DvVZPdl908Ab9-_rifL4rl7fXN_GpZGIppX3iHOVVWe-aY9wJhTBz3RFfCaoesoJxxQ0qPbVXZGcekZNQYbe2MllojQ0_A923v-OtmcLmXbcjTWyq6bshSsFnFmCDVSJ5vSZO6nJPzcp1Cq9KzxEhOYuUkVm7FjvTZrnfQrbP_2A-TI_BtC3TD-j9NX3d3V12sN6M7qZV59KFxkjJOCGOcvgOKm48U</recordid><startdate>20100701</startdate><enddate>20100701</enddate><creator>Song, Dongli</creator><creator>Liu, Xiaoyu</creator><creator>Liu, Rujiao</creator><creator>Yang, Ling</creator><creator>Zuo, Ji</creator><creator>Liu, Wen</creator><general>Oxford University Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100701</creationdate><title>Connexin 43 hemichannel regulates H9c2 cell proliferation by modulating intracellular ATP and [Ca^2＋]</title><author>Song, Dongli ; Liu, Xiaoyu ; Liu, Rujiao ; Yang, Ling ; Zuo, Ji ; Liu, Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-fe163adbf4e4ff70112e6f2b97dbe0d73646c25f1d99d8612543ccbdd835bb0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>ATP</topic><topic>Blotting, Western</topic><topic>Calcium - metabolism</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>Cell Survival - drug effects</topic><topic>Connexin 43 - genetics</topic><topic>Connexin 43 - metabolism</topic><topic>Connexin 43 - physiology</topic><topic>Cx43</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fluorescence Recovery After Photobleaching</topic><topic>Glycyrrhetinic Acid - analogs & derivatives</topic><topic>Glycyrrhetinic Acid - pharmacology</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - drug effects</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Wnt Proteins - genetics</topic><topic>Wnt Proteins - metabolism</topic><topic>Wnt3 Protein</topic><topic>心肌细胞</topic><topic>细胞内</topic><topic>细胞增殖</topic><topic>细胞活性</topic><topic>细胞间隙连接通讯</topic><topic>连接蛋白</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Dongli</creatorcontrib><creatorcontrib>Liu, Xiaoyu</creatorcontrib><creatorcontrib>Liu, Rujiao</creatorcontrib><creatorcontrib>Yang, Ling</creatorcontrib><creatorcontrib>Zuo, Ji</creatorcontrib><creatorcontrib>Liu, Wen</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Dongli</au><au>Liu, Xiaoyu</au><au>Liu, Rujiao</au><au>Yang, Ling</au><au>Zuo, Ji</au><au>Liu, Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Connexin 43 hemichannel regulates H9c2 cell proliferation by modulating intracellular ATP and [Ca^2＋]</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><date>2010-07-01</date><risdate>2010</risdate><volume>42</volume><issue>7</issue><spage>472</spage><epage>482</epage><pages>472-482</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>Connexin 43 （Cx43）, known to be the main protein building blocks of gap junctions and hemichannels in mammalian heart, plays an important role in cardiocytes proliferation. Gap junctional intercellular communication has been suggested to be necessary for cellular proliferation and differentiation. However, the effect of Cx43 hemichannel on cardiocytes proliferation and the mechanism remain unclear. In this study, rat heart cell line H9c2 was used. The Cx43 location, the proliferation rate and hemichannel activity of H9c2 cells and Wnt-3a^＋-H9c2 cells were investigated and the changes of intraceilular ATP and [Ca^2＋] were determined. Results showed that the inhibited hemichannel induced by 18β-glycyrrhetinic acid （GA） evoked intracellular ATP and [Ca^2＋] increase and enhanced H9c2 cell proliferation. Wnt-3a＋-H9c2 cells displayed enhanced hemichannel activity and proliferation rate. Inhibited hemichannel of Wnt-3a＋-H9c2 cells induced by 18β-GA decreased intracellular ATP, increased [Ca^2＋], and enhanced the proliferation of H9c2 cells. This study validated the role of hemichannel in H9c2 cell proliferation regulation, and showed a mechanism involved in the regulation of H9c2 cell proliferation. The proliferation could be enhanced by Cx43 hemichannel-mediated ATP release accompanying intraceilular [Ca^2＋]change. However, different changes of ATP were observed in Wnt-3a＋-H9c2 cells. These findings provided new insights into the molecular mechanisms of proliferation regulation in H9c2 cells and the effect of Wnt-3a on intraceliular ATP.</abstract><cop>China</cop><pub>Oxford University Press</pub><pmid>20705586</pmid><doi>10.1093/abbs/gmq047</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Triphosphate - metabolism Animals ATP Blotting, Western Calcium - metabolism Cell Line Cell Proliferation Cell Survival - drug effects Connexin 43 - genetics Connexin 43 - metabolism Connexin 43 - physiology Cx43 Dose-Response Relationship, Drug Fluorescence Recovery After Photobleaching Glycyrrhetinic Acid - analogs & derivatives Glycyrrhetinic Acid - pharmacology Myocytes, Cardiac - cytology Myocytes, Cardiac - drug effects Myocytes, Cardiac - metabolism Rats Reverse Transcriptase Polymerase Chain Reaction Wnt Proteins - genetics Wnt Proteins - metabolism Wnt3 Protein 心肌细胞 细胞内 细胞增殖 细胞活性 细胞间隙连接通讯 连接蛋白 |
| title | Connexin 43 hemichannel regulates H9c2 cell proliferation by modulating intracellular ATP and [Ca^2＋] |
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