Applications of the EYTEX™ method

The EYTEX™ method is an in vitro test to predict the ocular irritation of chemicals and formulations based on alterations in a protein matrix. Results of a multi-laboratory study of 130 samples have previously been published. Four independent studies were conducted to evaluate further the accuracy,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Toxicology in vitro 1990, Vol.4 (4), p.314-317
Hauptverfasser: Gordon, V.C., Kelly, C.P., Bergman, H.C.
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 317
container_issue 4
container_start_page 314
container_title Toxicology in vitro
container_volume 4
creator Gordon, V.C.
Kelly, C.P.
Bergman, H.C.
description The EYTEX™ method is an in vitro test to predict the ocular irritation of chemicals and formulations based on alterations in a protein matrix. Results of a multi-laboratory study of 130 samples have previously been published. Four independent studies were conducted to evaluate further the accuracy, reproducibility and applicability of the EYTEX™ method. This method was performed according to the previously published procedures. Dose-response curves were produced at concentrations of samples similar to those used in the Draize test. Spectrophotometric data were used to establish irritancy classifications, and an EYTEX™/Draize equivalent was calculated. These studies included a wide range of chemicals and formulations with varying degrees of irritation and mechanisms of ocular toxicity. Nine hundred and twenty-one cosmetic, household, petroleum and agricultural products were analysed by the EYTEX™ method. Seven hundred and thirty-six EYTEX™ results were within one classification of the Draize result. The EYTEX™ results exhibited an overall substantial equivalence of 91% to the Draize results with a range of 89 to 93% depending on the particular product group. The inter-assay coefficient of variation was 8.1% and the intra-assay coefficient of variation was 10.7%. These studies indicate that the EYTEX™ method exhibits a high correlation with the Draize test for the four diverse product groups studied and validates the predictive potential of the EYTEX™ method as an in vitro screen for ocular irritation.
doi_str_mv 10.1016/0887-2333(90)90071-Z
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_748942964</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>088723339090071Z</els_id><sourcerecordid>15861625</sourcerecordid><originalsourceid>FETCH-LOGICAL-c305t-ebfe29b06a71e379d35bdeb898741063144c601938fc6adb901e0aa4025f0f653</originalsourceid><addsrcrecordid>eNp9kMtKw0AUhgdRbL28gUhA8LKInrlkLhuhlHqBgpsK2s2QTE5oJGlqJhXc-yQ-mk9iYmuXXZ3N938HPkJOKFxToPIGtFYh45xfGrgyAIqG0x3Sp1qZkFOldkl_g_TIgfdvABBpBvukx0ABozrqk7PBYlHkLm7yau6DKguaGQaj18no5efrOyixmVXpEdnL4sLj8foekue70WT4EI6f7h-Hg3HoOERNiEmGzCQgY0WRK5PyKEkx0UYrQUFyKoSTQA3XmZNxmhigCHEsgEUZZDLih-Ri5V3U1fsSfWPL3DssiniO1dJbJbQRzEjRkudbSRppSSXrlGIFurryvsbMLuq8jOtPS8F2GW3XyHaNrAH7l9FO29np2r9MSkw3o_9uLXC7ArDt8ZFjbb3Lce4wzWt0jU2rfPuHX__df6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15861625</pqid></control><display><type>article</type><title>Applications of the EYTEX™ method</title><source>ScienceDirect Journals (5 years ago - present)</source><creator>Gordon, V.C. ; Kelly, C.P. ; Bergman, H.C.</creator><contributor>Parish, WE ; Hard, GC (eds)</contributor><creatorcontrib>Gordon, V.C. ; Kelly, C.P. ; Bergman, H.C. ; Parish, WE ; Hard, GC (eds)</creatorcontrib><description>The EYTEX™ method is an in vitro test to predict the ocular irritation of chemicals and formulations based on alterations in a protein matrix. Results of a multi-laboratory study of 130 samples have previously been published. Four independent studies were conducted to evaluate further the accuracy, reproducibility and applicability of the EYTEX™ method. This method was performed according to the previously published procedures. Dose-response curves were produced at concentrations of samples similar to those used in the Draize test. Spectrophotometric data were used to establish irritancy classifications, and an EYTEX™/Draize equivalent was calculated. These studies included a wide range of chemicals and formulations with varying degrees of irritation and mechanisms of ocular toxicity. Nine hundred and twenty-one cosmetic, household, petroleum and agricultural products were analysed by the EYTEX™ method. Seven hundred and thirty-six EYTEX™ results were within one classification of the Draize result. The EYTEX™ results exhibited an overall substantial equivalence of 91% to the Draize results with a range of 89 to 93% depending on the particular product group. The inter-assay coefficient of variation was 8.1% and the intra-assay coefficient of variation was 10.7%. These studies indicate that the EYTEX™ method exhibits a high correlation with the Draize test for the four diverse product groups studied and validates the predictive potential of the EYTEX™ method as an in vitro screen for ocular irritation.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/0887-2333(90)90071-Z</identifier><identifier>PMID: 20702185</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><ispartof>Toxicology in vitro, 1990, Vol.4 (4), p.314-317</ispartof><rights>1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-ebfe29b06a71e379d35bdeb898741063144c601938fc6adb901e0aa4025f0f653</citedby><cites>FETCH-LOGICAL-c305t-ebfe29b06a71e379d35bdeb898741063144c601938fc6adb901e0aa4025f0f653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0887-2333(90)90071-Z$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,4024,23930,23931,25140,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20702185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Parish, WE</contributor><contributor>Hard, GC (eds)</contributor><creatorcontrib>Gordon, V.C.</creatorcontrib><creatorcontrib>Kelly, C.P.</creatorcontrib><creatorcontrib>Bergman, H.C.</creatorcontrib><title>Applications of the EYTEX™ method</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>The EYTEX™ method is an in vitro test to predict the ocular irritation of chemicals and formulations based on alterations in a protein matrix. Results of a multi-laboratory study of 130 samples have previously been published. Four independent studies were conducted to evaluate further the accuracy, reproducibility and applicability of the EYTEX™ method. This method was performed according to the previously published procedures. Dose-response curves were produced at concentrations of samples similar to those used in the Draize test. Spectrophotometric data were used to establish irritancy classifications, and an EYTEX™/Draize equivalent was calculated. These studies included a wide range of chemicals and formulations with varying degrees of irritation and mechanisms of ocular toxicity. Nine hundred and twenty-one cosmetic, household, petroleum and agricultural products were analysed by the EYTEX™ method. Seven hundred and thirty-six EYTEX™ results were within one classification of the Draize result. The EYTEX™ results exhibited an overall substantial equivalence of 91% to the Draize results with a range of 89 to 93% depending on the particular product group. The inter-assay coefficient of variation was 8.1% and the intra-assay coefficient of variation was 10.7%. These studies indicate that the EYTEX™ method exhibits a high correlation with the Draize test for the four diverse product groups studied and validates the predictive potential of the EYTEX™ method as an in vitro screen for ocular irritation.</description><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNp9kMtKw0AUhgdRbL28gUhA8LKInrlkLhuhlHqBgpsK2s2QTE5oJGlqJhXc-yQ-mk9iYmuXXZ3N938HPkJOKFxToPIGtFYh45xfGrgyAIqG0x3Sp1qZkFOldkl_g_TIgfdvABBpBvukx0ABozrqk7PBYlHkLm7yau6DKguaGQaj18no5efrOyixmVXpEdnL4sLj8foekue70WT4EI6f7h-Hg3HoOERNiEmGzCQgY0WRK5PyKEkx0UYrQUFyKoSTQA3XmZNxmhigCHEsgEUZZDLih-Ri5V3U1fsSfWPL3DssiniO1dJbJbQRzEjRkudbSRppSSXrlGIFurryvsbMLuq8jOtPS8F2GW3XyHaNrAH7l9FO29np2r9MSkw3o_9uLXC7ArDt8ZFjbb3Lce4wzWt0jU2rfPuHX__df6g</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Gordon, V.C.</creator><creator>Kelly, C.P.</creator><creator>Bergman, H.C.</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Applications of the EYTEX™ method</title><author>Gordon, V.C. ; Kelly, C.P. ; Bergman, H.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-ebfe29b06a71e379d35bdeb898741063144c601938fc6adb901e0aa4025f0f653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gordon, V.C.</creatorcontrib><creatorcontrib>Kelly, C.P.</creatorcontrib><creatorcontrib>Bergman, H.C.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gordon, V.C.</au><au>Kelly, C.P.</au><au>Bergman, H.C.</au><au>Parish, WE</au><au>Hard, GC (eds)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Applications of the EYTEX™ method</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>1990</date><risdate>1990</risdate><volume>4</volume><issue>4</issue><spage>314</spage><epage>317</epage><pages>314-317</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><abstract>The EYTEX™ method is an in vitro test to predict the ocular irritation of chemicals and formulations based on alterations in a protein matrix. Results of a multi-laboratory study of 130 samples have previously been published. Four independent studies were conducted to evaluate further the accuracy, reproducibility and applicability of the EYTEX™ method. This method was performed according to the previously published procedures. Dose-response curves were produced at concentrations of samples similar to those used in the Draize test. Spectrophotometric data were used to establish irritancy classifications, and an EYTEX™/Draize equivalent was calculated. These studies included a wide range of chemicals and formulations with varying degrees of irritation and mechanisms of ocular toxicity. Nine hundred and twenty-one cosmetic, household, petroleum and agricultural products were analysed by the EYTEX™ method. Seven hundred and thirty-six EYTEX™ results were within one classification of the Draize result. The EYTEX™ results exhibited an overall substantial equivalence of 91% to the Draize results with a range of 89 to 93% depending on the particular product group. The inter-assay coefficient of variation was 8.1% and the intra-assay coefficient of variation was 10.7%. These studies indicate that the EYTEX™ method exhibits a high correlation with the Draize test for the four diverse product groups studied and validates the predictive potential of the EYTEX™ method as an in vitro screen for ocular irritation.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>20702185</pmid><doi>10.1016/0887-2333(90)90071-Z</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0887-2333
ispartof Toxicology in vitro, 1990, Vol.4 (4), p.314-317
issn 0887-2333
1879-3177
language eng
recordid cdi_proquest_miscellaneous_748942964
source ScienceDirect Journals (5 years ago - present)
title Applications of the EYTEX™ method
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T15%3A47%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Applications%20of%20the%20EYTEX%E2%84%A2%20method&rft.jtitle=Toxicology%20in%20vitro&rft.au=Gordon,%20V.C.&rft.date=1990&rft.volume=4&rft.issue=4&rft.spage=314&rft.epage=317&rft.pages=314-317&rft.issn=0887-2333&rft.eissn=1879-3177&rft_id=info:doi/10.1016/0887-2333(90)90071-Z&rft_dat=%3Cproquest_cross%3E15861625%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15861625&rft_id=info:pmid/20702185&rft_els_id=088723339090071Z&rfr_iscdi=true