Adult Rat Lung in Organ Culture: Maintenance of Histotypic Structure and Ability to Synthesize Phospholipid
The maintenance of adult rat lung explants in organ culture was assessed both morphologically and biochemically. A comparison of several culture media indicated that Ham's F12K plus 0.1 µM dexamethasone, which maintained the explants for 14 days, was superior. The ability of the explants to syn...
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| Veröffentlicht in: | In Vitro 1979-12, Vol.15 (12), p.1023-1031 |
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| container_issue | 12 |
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| container_title | In Vitro |
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| creator | Paul A. Weinhold Burkel, William E. Theodore V. Fischer Kahn, Raymond H. |
| description | The maintenance of adult rat lung explants in organ culture was assessed both morphologically and biochemically. A comparison of several culture media indicated that Ham's F12K plus 0.1 µM dexamethasone, which maintained the explants for 14 days, was superior. The ability of the explants to synthesize dipalmitoylphosphatidylcholine increased with the length of cultivation to values greater than the noncultivated controls. The DNA content remained constant for 7 days, and a relatively normal structural relationship between type I and type II pneumocytes was maintained. Explants cultivated in Ham's F12K without dexamethasone did not maintain a histotypic morphology; the type II pneumocytes appeared to proliferate and the ability to synthesize phosphatidylcholine decreased. |
| doi_str_mv | 10.1007/BF02619161 |
| format | Article |
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Explants cultivated in Ham's F12K without dexamethasone did not maintain a histotypic morphology; the type II pneumocytes appeared to proliferate and the ability to synthesize phosphatidylcholine decreased.</description><identifier>ISSN: 0073-5655</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/BF02619161</identifier><identifier>PMID: 535907</identifier><language>eng</language><publisher>United States: Tissue Culture Association, Inc</publisher><subject>Animals ; Biochemistry ; Culture Media ; Dexamethasone - pharmacology ; DNA - metabolism ; Female ; Lipids ; Lung - cytology ; Lung - metabolism ; Lungs ; Organ Culture Techniques ; Phosphatidylcholines ; Phosphatidylcholines - biosynthesis ; Phospholipids ; Plants ; Pneumocytes ; Pulmonary alveoli ; Radioactive decay ; Rats</subject><ispartof>In Vitro, 1979-12, Vol.15 (12), p.1023-1031</ispartof><rights>Copyright 1979 Tissue Culture Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c302t-ee88fb17eb49c3d87118cbf6b2c308968360a1d5ab2856af331f0b9723783993</citedby><cites>FETCH-LOGICAL-c302t-ee88fb17eb49c3d87118cbf6b2c308968360a1d5ab2856af331f0b9723783993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4292317$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4292317$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/535907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paul A. Weinhold</creatorcontrib><creatorcontrib>Burkel, William E.</creatorcontrib><creatorcontrib>Theodore V. Fischer</creatorcontrib><creatorcontrib>Kahn, Raymond H.</creatorcontrib><title>Adult Rat Lung in Organ Culture: Maintenance of Histotypic Structure and Ability to Synthesize Phospholipid</title><title>In Vitro</title><addtitle>In Vitro</addtitle><description>The maintenance of adult rat lung explants in organ culture was assessed both morphologically and biochemically. A comparison of several culture media indicated that Ham's F12K plus 0.1 µM dexamethasone, which maintained the explants for 14 days, was superior. The ability of the explants to synthesize dipalmitoylphosphatidylcholine increased with the length of cultivation to values greater than the noncultivated controls. The DNA content remained constant for 7 days, and a relatively normal structural relationship between type I and type II pneumocytes was maintained. Explants cultivated in Ham's F12K without dexamethasone did not maintain a histotypic morphology; the type II pneumocytes appeared to proliferate and the ability to synthesize phosphatidylcholine decreased.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Culture Media</subject><subject>Dexamethasone - pharmacology</subject><subject>DNA - metabolism</subject><subject>Female</subject><subject>Lipids</subject><subject>Lung - cytology</subject><subject>Lung - metabolism</subject><subject>Lungs</subject><subject>Organ Culture Techniques</subject><subject>Phosphatidylcholines</subject><subject>Phosphatidylcholines - biosynthesis</subject><subject>Phospholipids</subject><subject>Plants</subject><subject>Pneumocytes</subject><subject>Pulmonary alveoli</subject><subject>Radioactive decay</subject><subject>Rats</subject><issn>0073-5655</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkLtOwzAUQC3EqxQWZgZPDEgBPxI_2EpFKVJREe0eOYnTuqROsJ0hfD2pWtHpSvcc3SsdAG4xesQI8aeXCSIMS8zwCRjgmCcRYUKegkEPaZSwJLkEV95vEKKIEXwBzhOaSMQH4HtUtFWAXyrAWWtX0Fg4dytl4bhft04_ww9lbNBW2VzDuoRT40MdusbkcBFcm-8kqGwBR5mpTOhgqOGis2GtvfnV8HNd-2ZdV6YxxTU4K1Xl9c1hDsFy8rocT6PZ_O19PJpFOUUkRFoLUWaY6yyWOS0Ex1jkWcky0nMhmaAMKVwkKiMiYaqkFJcok5xQLqiUdAju92cbV_-02od0a3yuq0pZXbc-5bGQhMaiFx_2Yu5q750u08aZrXJdilG665oeu_by3eFqm2118a_uQx7xps_j_mlM-leY0z_bFHvg</recordid><startdate>197912</startdate><enddate>197912</enddate><creator>Paul A. 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Fischer ; Kahn, Raymond H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-ee88fb17eb49c3d87118cbf6b2c308968360a1d5ab2856af331f0b9723783993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Culture Media</topic><topic>Dexamethasone - pharmacology</topic><topic>DNA - metabolism</topic><topic>Female</topic><topic>Lipids</topic><topic>Lung - cytology</topic><topic>Lung - metabolism</topic><topic>Lungs</topic><topic>Organ Culture Techniques</topic><topic>Phosphatidylcholines</topic><topic>Phosphatidylcholines - biosynthesis</topic><topic>Phospholipids</topic><topic>Plants</topic><topic>Pneumocytes</topic><topic>Pulmonary alveoli</topic><topic>Radioactive decay</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paul A. Weinhold</creatorcontrib><creatorcontrib>Burkel, William E.</creatorcontrib><creatorcontrib>Theodore V. Fischer</creatorcontrib><creatorcontrib>Kahn, Raymond H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In Vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paul A. Weinhold</au><au>Burkel, William E.</au><au>Theodore V. Fischer</au><au>Kahn, Raymond H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adult Rat Lung in Organ Culture: Maintenance of Histotypic Structure and Ability to Synthesize Phospholipid</atitle><jtitle>In Vitro</jtitle><addtitle>In Vitro</addtitle><date>1979-12</date><risdate>1979</risdate><volume>15</volume><issue>12</issue><spage>1023</spage><epage>1031</epage><pages>1023-1031</pages><issn>0073-5655</issn><eissn>1475-2689</eissn><abstract>The maintenance of adult rat lung explants in organ culture was assessed both morphologically and biochemically. A comparison of several culture media indicated that Ham's F12K plus 0.1 µM dexamethasone, which maintained the explants for 14 days, was superior. The ability of the explants to synthesize dipalmitoylphosphatidylcholine increased with the length of cultivation to values greater than the noncultivated controls. The DNA content remained constant for 7 days, and a relatively normal structural relationship between type I and type II pneumocytes was maintained. Explants cultivated in Ham's F12K without dexamethasone did not maintain a histotypic morphology; the type II pneumocytes appeared to proliferate and the ability to synthesize phosphatidylcholine decreased.</abstract><cop>United States</cop><pub>Tissue Culture Association, Inc</pub><pmid>535907</pmid><doi>10.1007/BF02619161</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0073-5655 |
| ispartof | In Vitro, 1979-12, Vol.15 (12), p.1023-1031 |
| issn | 0073-5655 1475-2689 |
| language | eng |
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| source | MEDLINE; Jstor Complete Legacy; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biochemistry Culture Media Dexamethasone - pharmacology DNA - metabolism Female Lipids Lung - cytology Lung - metabolism Lungs Organ Culture Techniques Phosphatidylcholines Phosphatidylcholines - biosynthesis Phospholipids Plants Pneumocytes Pulmonary alveoli Radioactive decay Rats |
| title | Adult Rat Lung in Organ Culture: Maintenance of Histotypic Structure and Ability to Synthesize Phospholipid |
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