An Ultramicro Enzymatic Method for the Fluorimetric Determination of Uric Acid in Serum

An Ultramicro Enzymatic Method for the Fluorimetric Determination of Uric Acid in Serum

A highly sensitive fluorimetric method is presented for the determination of uric acid in serum, based on the uricase-catalized oxidation of the acid to hydrogen peroxide, which is measured by a fluorimetric technique using the peroxidase-p-hydroxyphenylacetic acid system. The method is rapidly and...

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Veröffentlicht in:Chemical & pharmaceutical bulletin 1979/09/25, Vol.27(9), pp.2245-2248
Hauptverfasser: NAGAI, HIDEAKI, ZAITSU, KIYOSHI, HAMADA, CHIZUKO, OHKURA, YOSUKE
Format: Artikel
Sprache:eng
Schlagworte:
Humans
Methods
Microchemistry
Spectrometry, Fluorescence
ultramicro analysis
Urate Oxidase
Uric Acid - blood
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Zusammenfassung:A highly sensitive fluorimetric method is presented for the determination of uric acid in serum, based on the uricase-catalized oxidation of the acid to hydrogen peroxide, which is measured by a fluorimetric technique using the peroxidase-p-hydroxyphenylacetic acid system. The method is rapidly and readily performed with only 2μl of serum without deproteinization.
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.27.2245