Effect of Centrifuge Speed on the Sedimentation of High-Molecular-Weight Bacteriophage G DNA

Effect of Centrifuge Speed on the Sedimentation of High-Molecular-Weight Bacteriophage G DNA

Large molecular weight bacteriophage G DNA, about five times larger than T2 DNA, was used to test Zimm's theory [(1974) Biophys. Chem. 1, 279–291] for the effect of rotor speed on the sedimentation of large linear monodisperse DNA. Sedimentation profiles from neutral sucrose gradinets at low an...

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Veröffentlicht in:Biopolymers 1979-09, Vol.18 (9), p.2353-2356
1. Verfasser: Krasin, Frank
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Sprache:eng
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Bacteriophages
Centrifugation, Density Gradient
Chemical Precipitation
DNA, Viral - analysis
Molecular Weight
T-Phages
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description Large molecular weight bacteriophage G DNA, about five times larger than T2 DNA, was used to test Zimm's theory [(1974) Biophys. Chem. 1, 279–291] for the effect of rotor speed on the sedimentation of large linear monodisperse DNA. Sedimentation profiles from neutral sucrose gradinets at low and high rotor speeds show G DNA sedimenting from 1.8 to 0.7 times as fast as T2 DNA. Experimental measurements indicate that the sedimentation coefficient of G DNA decreases with increasing rotor speed about as fast as predicted by theory.
doi_str_mv 10.1002/bip.1979.360180921
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subjects Bacteriophages
Centrifugation, Density Gradient
Chemical Precipitation
DNA, Viral - analysis
Molecular Weight
T-Phages
title Effect of Centrifuge Speed on the Sedimentation of High-Molecular-Weight Bacteriophage G DNA
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