Discontinuous translation of silk fibroin in a reticulocyte cell-free system and in intact silk gland cells [Bombyx mori]

Silk fibroin mRNA was translated in a rabbit reticulocyte cell-free system. Addition of tRNA from silk glands was essential for complete translation of the fibroin polypeptide. (M$_{\text{r}}\approx $400,000). Synthesis of full-sized product took at least 85 min. In addition to full-size product, a...

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