Feulgen cytophotometry of pine [Pinus rigida, Pinus lambertiana] nuclei. II. Effect of pectinase used in cell separation

Feulgen cytophotometry of pine [Pinus rigida, Pinus lambertiana] nuclei. II. Effect of pectinase used in cell separation

Pectinase used for cell separation prior to cytophotometry contains a DNase that is able to penetrate the cells of pine root tips and attack nuclear DNA. When pine root tips were exposed to 1% pectinase (pH 6.0), there was a decrease in nuclear DNA content at every sample point and a sharp drop betw...

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Veröffentlicht in:Stain technology 1979, Vol.54 (4), p.201-204
Hauptverfasser: Berlyn, G.P, Dhillon, S.S, Miksche, J.P
Format: Artikel
Sprache:eng
Schlagworte:
Cell Separation - methods
Deoxyribonucleases - pharmacology
DNA - analysis
Glycoside Hydrolases - pharmacology
Photometry - instrumentation
Photometry - methods
Pinus lambertiana
Pinus rigida
Polygalacturonase - pharmacology
Seeds - analysis
Seeds - cytology
Seeds - drug effects
Staining and Labeling - methods
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Zusammenfassung:Pectinase used for cell separation prior to cytophotometry contains a DNase that is able to penetrate the cells of pine root tips and attack nuclear DNA. When pine root tips were exposed to 1% pectinase (pH 6.0), there was a decrease in nuclear DNA content at every sample point and a sharp drop between 16 and 20 hr. The effect of the DNase was eliminated by preparing the enzyme solution in 0.01 M sodium citrate or 0.001 M EDTA. It is suggested that heat denaturation of the DNase should also be effective and might be used in combination with the magnesium chelators.
ISSN:0038-9153