$Effect of calcium on the cyclic GMP elevation induced by thymosin fraction 5$

Effect of calcium on the cyclic GMP elevation induced by thymosin fraction 5

Thymosin fraction 5 induces an increase in cyclic GMP but not cAMP in murine thymocytes. Calcium (0.6 mM) is necessary for an optimal response in both phosphate buffered saline and hepes-buffered RPMI 1640 media. The calcium dependence of the cGMP response was most pronounced in a minimal salts medi...

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Veröffentlicht in:	Biochemical and biophysical research communications 1979-10, Vol.90 (3), p.810-818
Hauptverfasser:	Naylor, Paul H., Thurman, Gary B., Goldstein, Allan L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological Transport - drug effects Calcium - pharmacology Concanavalin A Cyclic AMP - metabolism Cyclic GMP - metabolism Kinetics Lymphocyte Activation Mice T-Lymphocytes - metabolism Thymosin - pharmacology Thymus Gland - drug effects Thymus Gland - metabolism Thymus Hormones - pharmacology
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container_title	Biochemical and biophysical research communications
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creator	Naylor, Paul H. Thurman, Gary B. Goldstein, Allan L.
description	Thymosin fraction 5 induces an increase in cyclic GMP but not cAMP in murine thymocytes. Calcium (0.6 mM) is necessary for an optimal response in both phosphate buffered saline and hepes-buffered RPMI 1640 media. The calcium dependence of the cGMP response was most pronounced in a minimal salts medium (PBS) and higher concentrations (greater than 0.8 mM) caused a lessening of the cGMP elevation induced by thymosin. Basal cGMP levels of thymus and spleen lymphocytes vary with increasing concentrations of calcium (0–1 mM) and to a lesser extent, the levels of cAMP also are increased. Calcium uptake was measured both at mitogenic levels of Con A and at thymosin concentrations which were similar to those necessary for the increase in cGMP. The results suggest that calcium and cGMP play an important role in T cell differentiation under the influence of thymosin.
doi_str_mv	10.1016/0006-291X(79)91900-4
format	Article
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Calcium (0.6 mM) is necessary for an optimal response in both phosphate buffered saline and hepes-buffered RPMI 1640 media. The calcium dependence of the cGMP response was most pronounced in a minimal salts medium (PBS) and higher concentrations (greater than 0.8 mM) caused a lessening of the cGMP elevation induced by thymosin. Basal cGMP levels of thymus and spleen lymphocytes vary with increasing concentrations of calcium (0–1 mM) and to a lesser extent, the levels of cAMP also are increased. Calcium uptake was measured both at mitogenic levels of Con A and at thymosin concentrations which were similar to those necessary for the increase in cGMP. 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Calcium (0.6 mM) is necessary for an optimal response in both phosphate buffered saline and hepes-buffered RPMI 1640 media. The calcium dependence of the cGMP response was most pronounced in a minimal salts medium (PBS) and higher concentrations (greater than 0.8 mM) caused a lessening of the cGMP elevation induced by thymosin. Basal cGMP levels of thymus and spleen lymphocytes vary with increasing concentrations of calcium (0–1 mM) and to a lesser extent, the levels of cAMP also are increased. Calcium uptake was measured both at mitogenic levels of Con A and at thymosin concentrations which were similar to those necessary for the increase in cGMP. The results suggest that calcium and cGMP play an important role in T cell differentiation under the influence of thymosin.</description><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Calcium - pharmacology</subject><subject>Concanavalin A</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic GMP - metabolism</subject><subject>Kinetics</subject><subject>Lymphocyte Activation</subject><subject>Mice</subject><subject>T-Lymphocytes - metabolism</subject><subject>Thymosin - pharmacology</subject><subject>Thymus Gland - drug effects</subject><subject>Thymus Gland - metabolism</subject><subject>Thymus Hormones - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEURoP4qtV_0EVWoovRm8wzG0FKrUJFFwruQh43GJlHncwU-u-dPujSVRbnuwdyCJkwuGPAsnsAyCIu2NdNLm4FEwBRckRGDAREnEFyTEaHyTm5COEHgLEkE2fklPMiy2FEFjPn0HS0cdSo0vi-ok1Nu2-kZm1Kb-j89Z1iiSvV-QH42vYGLdXrYbOumuBr6lpltjC9JCdOlQGv9u-YfD7NPqbP0eJt_jJ9XEQmTrMuYnEex86mWqvYFAqLXPEiZ6jBpU5rZOCU0ImDIuVKWG7y1FmnU-AF09Ym8Zhc77zLtvntMXSy8sFgWaoamz7IPCl4kcTxMEx2Q9M2IbTo5LL1lWrXkoHcNJSbQHITSOZCbhvKjX-y9_e6Qns42kUb8MMO4_DHlcdWBuOxHrr4dmgpbeP_9_8B1rSAqQ</recordid><startdate>19791012</startdate><enddate>19791012</enddate><creator>Naylor, Paul H.</creator><creator>Thurman, Gary B.</creator><creator>Goldstein, Allan L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19791012</creationdate><title>Effect of calcium on the cyclic GMP elevation induced by thymosin fraction 5</title><author>Naylor, Paul H. ; Thurman, Gary B. ; Goldstein, Allan L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-13733fd5bba3c8ae87a2871eb0f5fbbe10fa9b4f0852a9d2c75fdfb50281bdd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Calcium - pharmacology</topic><topic>Concanavalin A</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic GMP - metabolism</topic><topic>Kinetics</topic><topic>Lymphocyte Activation</topic><topic>Mice</topic><topic>T-Lymphocytes - metabolism</topic><topic>Thymosin - pharmacology</topic><topic>Thymus Gland - drug effects</topic><topic>Thymus Gland - metabolism</topic><topic>Thymus Hormones - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naylor, Paul H.</creatorcontrib><creatorcontrib>Thurman, Gary B.</creatorcontrib><creatorcontrib>Goldstein, Allan L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naylor, Paul H.</au><au>Thurman, Gary B.</au><au>Goldstein, Allan L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of calcium on the cyclic GMP elevation induced by thymosin fraction 5</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1979-10-12</date><risdate>1979</risdate><volume>90</volume><issue>3</issue><spage>810</spage><epage>818</epage><pages>810-818</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Thymosin fraction 5 induces an increase in cyclic GMP but not cAMP in murine thymocytes. 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subjects	Animals Biological Transport - drug effects Calcium - pharmacology Concanavalin A Cyclic AMP - metabolism Cyclic GMP - metabolism Kinetics Lymphocyte Activation Mice T-Lymphocytes - metabolism Thymosin - pharmacology Thymus Gland - drug effects Thymus Gland - metabolism Thymus Hormones - pharmacology
title	Effect of calcium on the cyclic GMP elevation induced by thymosin fraction 5
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