Establishment and characterization of a new endometrial cancer cell line (SCRC-1)
A new human endometrial cell line, SCRC-1, has been established from a uterine adenocarcinoma. During a period of 57 weeks in culture, the SCRC-1 cells have been passaged over 100 times. Cultures have an epithelioid morphology, high mitotic activity, and a tendency to form multiple cell layers. The...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1979-11, Vol.135 (5), p.671-679 |
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| container_issue | 5 |
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| container_title | American journal of obstetrics and gynecology |
| container_volume | 135 |
| creator | Gorodecki, Justine Mortel, Rodrigue Ladda, Roger L. Ward, Samuel P. Geder, Laszlo Rapp, Fred |
| description | A new human endometrial cell line, SCRC-1, has been established from a uterine adenocarcinoma. During a period of 57 weeks in culture, the SCRC-1 cells have been passaged over 100 times. Cultures have an epithelioid morphology, high mitotic activity, and a tendency to form multiple cell layers. The cells are polygonal and are arranged in pavement-like fashion. Most have multiple nucleoli, some are multinucleated, and all have heavily vacuolated cytoplasm. Pretreatment with iododeoxyuridine did not reveal viral markers by cocultivation or immunofluorescence techniques. Karyologic studies indicate that the SCRC-1 cell line is essentially diploid with a small subpopulation of tetraploid cells. Over a period of 20 weeks, 1 of 14 athymic nude mice inoculated with SCRC-1 cells developed a tumor which histologically closely resembled the original human tumor. Cells were subsequently propagated from this tumor and the resulting epithelioid-like cell line was designated SCRC-1, Tu 1. |
| doi_str_mv | 10.1016/S0002-9378(16)32994-5 |
| format | Article |
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During a period of 57 weeks in culture, the SCRC-1 cells have been passaged over 100 times. Cultures have an epithelioid morphology, high mitotic activity, and a tendency to form multiple cell layers. The cells are polygonal and are arranged in pavement-like fashion. Most have multiple nucleoli, some are multinucleated, and all have heavily vacuolated cytoplasm. Pretreatment with iododeoxyuridine did not reveal viral markers by cocultivation or immunofluorescence techniques. Karyologic studies indicate that the SCRC-1 cell line is essentially diploid with a small subpopulation of tetraploid cells. Over a period of 20 weeks, 1 of 14 athymic nude mice inoculated with SCRC-1 cells developed a tumor which histologically closely resembled the original human tumor. 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During a period of 57 weeks in culture, the SCRC-1 cells have been passaged over 100 times. Cultures have an epithelioid morphology, high mitotic activity, and a tendency to form multiple cell layers. The cells are polygonal and are arranged in pavement-like fashion. Most have multiple nucleoli, some are multinucleated, and all have heavily vacuolated cytoplasm. Pretreatment with iododeoxyuridine did not reveal viral markers by cocultivation or immunofluorescence techniques. Karyologic studies indicate that the SCRC-1 cell line is essentially diploid with a small subpopulation of tetraploid cells. Over a period of 20 weeks, 1 of 14 athymic nude mice inoculated with SCRC-1 cells developed a tumor which histologically closely resembled the original human tumor. Cells were subsequently propagated from this tumor and the resulting epithelioid-like cell line was designated SCRC-1, Tu 1.</description><subject>Adenocarcinoma - microbiology</subject><subject>Adenocarcinoma - pathology</subject><subject>Animals</subject><subject>Antigens, Neoplasm</subject><subject>Antigens, Viral</subject><subject>Cell Line</subject><subject>Cytomegalovirus - immunology</subject><subject>Epithelium - pathology</subject><subject>Female</subject><subject>Fibroblasts - pathology</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Kinetics</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Middle Aged</subject><subject>Simplexvirus - immunology</subject><subject>Trypsin</subject><subject>Uterine Neoplasms - microbiology</subject><subject>Uterine Neoplasms - pathology</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQx4P4qtVvoJCTtIfVJLvJZk8ipT6gIFo9h2x2QiP7qMlW0U9v-sCrp5lh_jP_mR9CF5RcUULF9ZwQwpIizeWIinHKiiJL-B4aUFLkiZBC7qPBn-QYnYTwvi5ZwY7QIWOScz5Az9PQ67J2YdFA22PdVtgstNemB-9-dO-6FncWa9zCF4a26hrovdM1Nro14LGBusa1awGP5pOXSULHp-jA6jrA2S4O0dvd9HXykMye7h8nt7PEpLzoE0krIIUlOdEkl9bG0wSVaRqTgtEqA2GFLUkpMuAyLfNMk4oYnTHDgNuyTIfocrt36buPFYReNS6sz9EtdKug8kzGxUxEId8Kje9C8GDV0rtG-29FiVqTVBuSao1JxWpDUvE4d74zWJUNVH9TW3SxfbNtQ3zy04FXwTiIUCrnwfSq6tw_Br8GM4G9</recordid><startdate>19791101</startdate><enddate>19791101</enddate><creator>Gorodecki, Justine</creator><creator>Mortel, Rodrigue</creator><creator>Ladda, Roger L.</creator><creator>Ward, Samuel P.</creator><creator>Geder, Laszlo</creator><creator>Rapp, Fred</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19791101</creationdate><title>Establishment and characterization of a new endometrial cancer cell line (SCRC-1)</title><author>Gorodecki, Justine ; Mortel, Rodrigue ; Ladda, Roger L. ; Ward, Samuel P. ; Geder, Laszlo ; Rapp, Fred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-81de09f070a078ff00061833f00921d4e6f6fb0b64e583b74a0d0ca42c2e5fbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Adenocarcinoma - microbiology</topic><topic>Adenocarcinoma - pathology</topic><topic>Animals</topic><topic>Antigens, Neoplasm</topic><topic>Antigens, Viral</topic><topic>Cell Line</topic><topic>Cytomegalovirus - immunology</topic><topic>Epithelium - pathology</topic><topic>Female</topic><topic>Fibroblasts - pathology</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Kinetics</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Middle Aged</topic><topic>Simplexvirus - immunology</topic><topic>Trypsin</topic><topic>Uterine Neoplasms - microbiology</topic><topic>Uterine Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gorodecki, Justine</creatorcontrib><creatorcontrib>Mortel, Rodrigue</creatorcontrib><creatorcontrib>Ladda, Roger L.</creatorcontrib><creatorcontrib>Ward, Samuel P.</creatorcontrib><creatorcontrib>Geder, Laszlo</creatorcontrib><creatorcontrib>Rapp, Fred</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gorodecki, Justine</au><au>Mortel, Rodrigue</au><au>Ladda, Roger L.</au><au>Ward, Samuel P.</au><au>Geder, Laszlo</au><au>Rapp, Fred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and characterization of a new endometrial cancer cell line (SCRC-1)</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>1979-11-01</date><risdate>1979</risdate><volume>135</volume><issue>5</issue><spage>671</spage><epage>679</epage><pages>671-679</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><abstract>A new human endometrial cell line, SCRC-1, has been established from a uterine adenocarcinoma. During a period of 57 weeks in culture, the SCRC-1 cells have been passaged over 100 times. Cultures have an epithelioid morphology, high mitotic activity, and a tendency to form multiple cell layers. The cells are polygonal and are arranged in pavement-like fashion. Most have multiple nucleoli, some are multinucleated, and all have heavily vacuolated cytoplasm. Pretreatment with iododeoxyuridine did not reveal viral markers by cocultivation or immunofluorescence techniques. Karyologic studies indicate that the SCRC-1 cell line is essentially diploid with a small subpopulation of tetraploid cells. Over a period of 20 weeks, 1 of 14 athymic nude mice inoculated with SCRC-1 cells developed a tumor which histologically closely resembled the original human tumor. 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| identifier | ISSN: 0002-9378 |
| ispartof | American journal of obstetrics and gynecology, 1979-11, Vol.135 (5), p.671-679 |
| issn | 0002-9378 1097-6868 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adenocarcinoma - microbiology Adenocarcinoma - pathology Animals Antigens, Neoplasm Antigens, Viral Cell Line Cytomegalovirus - immunology Epithelium - pathology Female Fibroblasts - pathology Humans Karyotyping Kinetics Mice Mice, Nude Middle Aged Simplexvirus - immunology Trypsin Uterine Neoplasms - microbiology Uterine Neoplasms - pathology |
| title | Establishment and characterization of a new endometrial cancer cell line (SCRC-1) |
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