A rapid radioenzymatic assay for dopamine and N-acetyldopamine
Dopamine (DA) was measured in various tissue extracts as [ 3H]methoxy- N-acetyldopamine after incubation with two partially purified enzymes, catechol- O-methyl transferase (EC 2.1.1.1) and N-acetyltransferase (EC 2.3.1.5), in the presence of [ 3H]adenosylmethionine and acetyl-CoA. This product can...
Gespeichert in:
| Veröffentlicht in: | Analytical biochemistry 1979-07, Vol.96 (1), p.175-180 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 180 |
|---|---|
| container_issue | 1 |
| container_start_page | 175 |
| container_title | Analytical biochemistry |
| container_volume | 96 |
| creator | McCaman, M.W. McCaman, R.E. Stetzler, J. |
| description | Dopamine (DA) was measured in various tissue extracts as [
3H]methoxy-
N-acetyldopamine after incubation with two partially purified enzymes, catechol-
O-methyl transferase (EC 2.1.1.1) and
N-acetyltransferase (EC 2.3.1.5), in the presence of [
3H]adenosylmethionine and acetyl-CoA. This product can be separated quantitatively from labeled products of norepinephrine and epinephrine by solvent extraction.
N-Acetyl-DA can be assayed by omitting the acetylating system from the incubation mixture. The procedure is rapid, convenient for processing large numbers of samples, and has a sensitivity of approximately 0.1 pmol. It has been used to measure DA in ganglia and in individual neurons from gastropod mollusks. |
| doi_str_mv | 10.1016/0003-2697(79)90570-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74768547</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0003269779905700</els_id><sourcerecordid>74768547</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-6ca2932900fbe8cd947f7c39da1d24acf08ece5ecd013c7db0d11ea94bb8a8083</originalsourceid><addsrcrecordid>eNp9kEtLxDAQx4P4Wle_wR56Ej1EJ23aNJeFZfEFi170HNJkCpG-TLpC_fR27erRywzM_wHzI2TB4IYBy24BIKFxJsWVkNcSUgEUDsiMgcwoJCAPyezPckrOQngHYIyn2Qk55jKVOZuR5SryunN2nNa12HwNte6diXQIeojK1ke27XTtGox0Y6Nnqg32Q_V7PCdHpa4CXuz3nLzd372uH-nm5eFpvdpQk6RZTzOjY5nEEqAsMDdWclEKk0irmY25NiXkaDBFY4ElRtgCLGOoJS-KXOeQJ3NyOfV2vv3YYuhV7YLBqtINttugBBdZnnIxGvlkNL4NwWOpOu9q7QfFQO2oqR0StUOihFQ_1BSMscW-f1vUaP9CE6ZRXk4yjj9-OvQqGIeNQes8ml7Z1v3f_w2OlHwK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74768547</pqid></control><display><type>article</type><title>A rapid radioenzymatic assay for dopamine and N-acetyldopamine</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>McCaman, M.W. ; McCaman, R.E. ; Stetzler, J.</creator><creatorcontrib>McCaman, M.W. ; McCaman, R.E. ; Stetzler, J.</creatorcontrib><description>Dopamine (DA) was measured in various tissue extracts as [
3H]methoxy-
N-acetyldopamine after incubation with two partially purified enzymes, catechol-
O-methyl transferase (EC 2.1.1.1) and
N-acetyltransferase (EC 2.3.1.5), in the presence of [
3H]adenosylmethionine and acetyl-CoA. This product can be separated quantitatively from labeled products of norepinephrine and epinephrine by solvent extraction.
N-Acetyl-DA can be assayed by omitting the acetylating system from the incubation mixture. The procedure is rapid, convenient for processing large numbers of samples, and has a sensitivity of approximately 0.1 pmol. It has been used to measure DA in ganglia and in individual neurons from gastropod mollusks.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(79)90570-0</identifier><identifier>PMID: 495981</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetyltransferases ; Animals ; Catechol O-Methyltransferase ; Dopamine - analogs & derivatives ; Dopamine - analysis ; Ganglia - analysis ; Mollusca ; Neurons - analysis ; Rats ; Substrate Specificity</subject><ispartof>Analytical biochemistry, 1979-07, Vol.96 (1), p.175-180</ispartof><rights>1979</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-6ca2932900fbe8cd947f7c39da1d24acf08ece5ecd013c7db0d11ea94bb8a8083</citedby><cites>FETCH-LOGICAL-c356t-6ca2932900fbe8cd947f7c39da1d24acf08ece5ecd013c7db0d11ea94bb8a8083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(79)90570-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/495981$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McCaman, M.W.</creatorcontrib><creatorcontrib>McCaman, R.E.</creatorcontrib><creatorcontrib>Stetzler, J.</creatorcontrib><title>A rapid radioenzymatic assay for dopamine and N-acetyldopamine</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Dopamine (DA) was measured in various tissue extracts as [
3H]methoxy-
N-acetyldopamine after incubation with two partially purified enzymes, catechol-
O-methyl transferase (EC 2.1.1.1) and
N-acetyltransferase (EC 2.3.1.5), in the presence of [
3H]adenosylmethionine and acetyl-CoA. This product can be separated quantitatively from labeled products of norepinephrine and epinephrine by solvent extraction.
N-Acetyl-DA can be assayed by omitting the acetylating system from the incubation mixture. The procedure is rapid, convenient for processing large numbers of samples, and has a sensitivity of approximately 0.1 pmol. It has been used to measure DA in ganglia and in individual neurons from gastropod mollusks.</description><subject>Acetyltransferases</subject><subject>Animals</subject><subject>Catechol O-Methyltransferase</subject><subject>Dopamine - analogs & derivatives</subject><subject>Dopamine - analysis</subject><subject>Ganglia - analysis</subject><subject>Mollusca</subject><subject>Neurons - analysis</subject><subject>Rats</subject><subject>Substrate Specificity</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQx4P4Wle_wR56Ej1EJ23aNJeFZfEFi170HNJkCpG-TLpC_fR27erRywzM_wHzI2TB4IYBy24BIKFxJsWVkNcSUgEUDsiMgcwoJCAPyezPckrOQngHYIyn2Qk55jKVOZuR5SryunN2nNa12HwNte6diXQIeojK1ke27XTtGox0Y6Nnqg32Q_V7PCdHpa4CXuz3nLzd372uH-nm5eFpvdpQk6RZTzOjY5nEEqAsMDdWclEKk0irmY25NiXkaDBFY4ElRtgCLGOoJS-KXOeQJ3NyOfV2vv3YYuhV7YLBqtINttugBBdZnnIxGvlkNL4NwWOpOu9q7QfFQO2oqR0StUOihFQ_1BSMscW-f1vUaP9CE6ZRXk4yjj9-OvQqGIeNQes8ml7Z1v3f_w2OlHwK</recordid><startdate>19790701</startdate><enddate>19790701</enddate><creator>McCaman, M.W.</creator><creator>McCaman, R.E.</creator><creator>Stetzler, J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19790701</creationdate><title>A rapid radioenzymatic assay for dopamine and N-acetyldopamine</title><author>McCaman, M.W. ; McCaman, R.E. ; Stetzler, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-6ca2932900fbe8cd947f7c39da1d24acf08ece5ecd013c7db0d11ea94bb8a8083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Acetyltransferases</topic><topic>Animals</topic><topic>Catechol O-Methyltransferase</topic><topic>Dopamine - analogs & derivatives</topic><topic>Dopamine - analysis</topic><topic>Ganglia - analysis</topic><topic>Mollusca</topic><topic>Neurons - analysis</topic><topic>Rats</topic><topic>Substrate Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McCaman, M.W.</creatorcontrib><creatorcontrib>McCaman, R.E.</creatorcontrib><creatorcontrib>Stetzler, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McCaman, M.W.</au><au>McCaman, R.E.</au><au>Stetzler, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid radioenzymatic assay for dopamine and N-acetyldopamine</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1979-07-01</date><risdate>1979</risdate><volume>96</volume><issue>1</issue><spage>175</spage><epage>180</epage><pages>175-180</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Dopamine (DA) was measured in various tissue extracts as [
3H]methoxy-
N-acetyldopamine after incubation with two partially purified enzymes, catechol-
O-methyl transferase (EC 2.1.1.1) and
N-acetyltransferase (EC 2.3.1.5), in the presence of [
3H]adenosylmethionine and acetyl-CoA. This product can be separated quantitatively from labeled products of norepinephrine and epinephrine by solvent extraction.
N-Acetyl-DA can be assayed by omitting the acetylating system from the incubation mixture. The procedure is rapid, convenient for processing large numbers of samples, and has a sensitivity of approximately 0.1 pmol. It has been used to measure DA in ganglia and in individual neurons from gastropod mollusks.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>495981</pmid><doi>10.1016/0003-2697(79)90570-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1979-07, Vol.96 (1), p.175-180 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74768547 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetyltransferases Animals Catechol O-Methyltransferase Dopamine - analogs & derivatives Dopamine - analysis Ganglia - analysis Mollusca Neurons - analysis Rats Substrate Specificity |
| title | A rapid radioenzymatic assay for dopamine and N-acetyldopamine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T16%3A55%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20rapid%20radioenzymatic%20assay%20for%20dopamine%20and%20N-acetyldopamine&rft.jtitle=Analytical%20biochemistry&rft.au=McCaman,%20M.W.&rft.date=1979-07-01&rft.volume=96&rft.issue=1&rft.spage=175&rft.epage=180&rft.pages=175-180&rft.issn=0003-2697&rft.eissn=1096-0309&rft_id=info:doi/10.1016/0003-2697(79)90570-0&rft_dat=%3Cproquest_cross%3E74768547%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74768547&rft_id=info:pmid/495981&rft_els_id=0003269779905700&rfr_iscdi=true |