An automated continuous flow procedure for the determination of enterokinase
A continuous flow method has been developed for the automatic determination of enterokinase in rat small intestine mucosa and/or luminal content. Trypsinogen was first hydrolysed by enterokinase under conditions which minimize autocatalytic activation. l-benzoyl-arginine paranitroanilide was then ad...
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| Veröffentlicht in: | Clinica chimica acta 1979-11, Vol.98 (3), p.187-194 |
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| container_title | Clinica chimica acta |
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| creator | Vaultier, J.P. Eloy, R. Hoeltzel, A. Grenier, J.F. |
| description | A continuous flow method has been developed for the automatic determination of enterokinase in rat small intestine mucosa and/or luminal content.
Trypsinogen was first hydrolysed by enterokinase under conditions which minimize autocatalytic activation.
l-benzoyl-arginine
paranitroanilide was then added and split to
paranitroaniline by the trypsin so formed. Liberated
paranitroalinine was diazotized and converted by the Bratton-Marshall reagent (
N-naphthyl ethylene diamine) to an azodye, with maximum absorption at 550 nm. This method of determination was found to be six times more sensitive than the direct
p-nitroaniline determination method. 36 determinations can be made hourly. |
| doi_str_mv | 10.1016/0009-8981(79)90144-X |
| format | Article |
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Trypsinogen was first hydrolysed by enterokinase under conditions which minimize autocatalytic activation.
l-benzoyl-arginine
paranitroanilide was then added and split to
paranitroaniline by the trypsin so formed. Liberated
paranitroalinine was diazotized and converted by the Bratton-Marshall reagent (
N-naphthyl ethylene diamine) to an azodye, with maximum absorption at 550 nm. This method of determination was found to be six times more sensitive than the direct
p-nitroaniline determination method. 36 determinations can be made hourly.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(79)90144-X</identifier><identifier>PMID: 40719</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Autoanalysis ; Bile Acids and Salts - pharmacology ; Calcium - pharmacology ; Clinical Enzyme Tests - methods ; Endopeptidases - analysis ; Enteropeptidase - analysis ; Hydrogen-Ion Concentration ; Intestinal Mucosa - enzymology ; Intestine, Small - enzymology ; Rats</subject><ispartof>Clinica chimica acta, 1979-11, Vol.98 (3), p.187-194</ispartof><rights>1979</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-86f7bd26324df7122142148c94c331edb9cb5c9df4f3119808f17be737355b283</citedby><cites>FETCH-LOGICAL-c355t-86f7bd26324df7122142148c94c331edb9cb5c9df4f3119808f17be737355b283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/000989817990144X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/40719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vaultier, J.P.</creatorcontrib><creatorcontrib>Eloy, R.</creatorcontrib><creatorcontrib>Hoeltzel, A.</creatorcontrib><creatorcontrib>Grenier, J.F.</creatorcontrib><title>An automated continuous flow procedure for the determination of enterokinase</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>A continuous flow method has been developed for the automatic determination of enterokinase in rat small intestine mucosa and/or luminal content.
Trypsinogen was first hydrolysed by enterokinase under conditions which minimize autocatalytic activation.
l-benzoyl-arginine
paranitroanilide was then added and split to
paranitroaniline by the trypsin so formed. Liberated
paranitroalinine was diazotized and converted by the Bratton-Marshall reagent (
N-naphthyl ethylene diamine) to an azodye, with maximum absorption at 550 nm. This method of determination was found to be six times more sensitive than the direct
p-nitroaniline determination method. 36 determinations can be made hourly.</description><subject>Animals</subject><subject>Autoanalysis</subject><subject>Bile Acids and Salts - pharmacology</subject><subject>Calcium - pharmacology</subject><subject>Clinical Enzyme Tests - methods</subject><subject>Endopeptidases - analysis</subject><subject>Enteropeptidase - analysis</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intestinal Mucosa - enzymology</subject><subject>Intestine, Small - enzymology</subject><subject>Rats</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LAzEQxYNYtFY_gR5yEj2sJpt0s7kIpfgPCl4Uegu7yQSj3Y0mWcVvb2pLj0JgyMx7j5kfQmeUXFFCq2tCiCxqWdMLIS8loZwXyz00prVgBeOy3EfjneQQHcX4lr-cVPQAjTgRVI7RYtbjZki-axIYrH2fXD_4IWK78t_4I3gNZgiArQ84vQI2kCB0rm-S8z32FkOfG_49dyIco5FtVhFOtnWCXu5un-cPxeLp_nE-WxSaTaepqCsrWlNWrOTGClqWlOdXa8k1YxRMK3U71dJYbhmlsia1paIFwUS2t2XNJuh8k5v3-xwgJtW5qGG1anrIuyvBBa9KVmUh3wh18DEGsOojuK4JP4oStUao1nzUmo8SUv0hVMtsO93mD20HZmf6Y5anN5sp5BO_HAQVtYM-g3IBdFLGu__jfwHKQIBV</recordid><startdate>19791102</startdate><enddate>19791102</enddate><creator>Vaultier, J.P.</creator><creator>Eloy, R.</creator><creator>Hoeltzel, A.</creator><creator>Grenier, J.F.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19791102</creationdate><title>An automated continuous flow procedure for the determination of enterokinase</title><author>Vaultier, J.P. ; Eloy, R. ; Hoeltzel, A. ; Grenier, J.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-86f7bd26324df7122142148c94c331edb9cb5c9df4f3119808f17be737355b283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Autoanalysis</topic><topic>Bile Acids and Salts - pharmacology</topic><topic>Calcium - pharmacology</topic><topic>Clinical Enzyme Tests - methods</topic><topic>Endopeptidases - analysis</topic><topic>Enteropeptidase - analysis</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intestinal Mucosa - enzymology</topic><topic>Intestine, Small - enzymology</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vaultier, J.P.</creatorcontrib><creatorcontrib>Eloy, R.</creatorcontrib><creatorcontrib>Hoeltzel, A.</creatorcontrib><creatorcontrib>Grenier, J.F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vaultier, J.P.</au><au>Eloy, R.</au><au>Hoeltzel, A.</au><au>Grenier, J.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An automated continuous flow procedure for the determination of enterokinase</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1979-11-02</date><risdate>1979</risdate><volume>98</volume><issue>3</issue><spage>187</spage><epage>194</epage><pages>187-194</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>A continuous flow method has been developed for the automatic determination of enterokinase in rat small intestine mucosa and/or luminal content.
Trypsinogen was first hydrolysed by enterokinase under conditions which minimize autocatalytic activation.
l-benzoyl-arginine
paranitroanilide was then added and split to
paranitroaniline by the trypsin so formed. Liberated
paranitroalinine was diazotized and converted by the Bratton-Marshall reagent (
N-naphthyl ethylene diamine) to an azodye, with maximum absorption at 550 nm. This method of determination was found to be six times more sensitive than the direct
p-nitroaniline determination method. 36 determinations can be made hourly.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>40719</pmid><doi>10.1016/0009-8981(79)90144-X</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1979-11, Vol.98 (3), p.187-194 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74746236 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Autoanalysis Bile Acids and Salts - pharmacology Calcium - pharmacology Clinical Enzyme Tests - methods Endopeptidases - analysis Enteropeptidase - analysis Hydrogen-Ion Concentration Intestinal Mucosa - enzymology Intestine, Small - enzymology Rats |
| title | An automated continuous flow procedure for the determination of enterokinase |
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