Immobilization of enzymes and microbial cells using carrageenan as matrix
Conditions for the gelation of k ‐carrageenan, which is a new polymer for immobilization of enzymes and microbial cells, were investigated in detail. k ‐Carrageenan was easily induced to gel by contact with metal ions, amines, amino acid derivatives, and water‐miscible organic solvents. By using thi...
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| Veröffentlicht in: | Biotechnology and bioengineering 1979-10, Vol.21 (10), p.1697-1709 |
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| container_issue | 10 |
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| container_title | Biotechnology and bioengineering |
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| creator | Tosa, Tetsuya Sato, Tadashi Mori, Takao Yamamoto, Kazo Takata, Isao Nishida, Yutaka Chibata, Ichiro |
| description | Conditions for the gelation of k ‐carrageenan, which is a new polymer for immobilization of enzymes and microbial cells, were investigated in detail. k ‐Carrageenan was easily induced to gel by contact with metal ions, amines, amino acid derivatives, and water‐miscible organic solvents. By using this property of k ‐carrageenan, the immobilization of enzymes and microbial cells was investigated. Several kinds of enzymes and microbial cells were easily immobilized with high enzyme activities. Immobilized preparations were easily tailor‐made to various shapes such as cube, bead, and membrane. The obtained immobilized preparations were stable, and columns packed with them were used for continuous enzyme reaction for a long period. Their operational stabilities were enhanced by hardening with glutaraldehyde and hexamethylenediamine. |
| doi_str_mv | 10.1002/bit.260211002 |
| format | Article |
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By using this property of k ‐carrageenan, the immobilization of enzymes and microbial cells was investigated. Several kinds of enzymes and microbial cells were easily immobilized with high enzyme activities. Immobilized preparations were easily tailor‐made to various shapes such as cube, bead, and membrane. The obtained immobilized preparations were stable, and columns packed with them were used for continuous enzyme reaction for a long period. 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Their operational stabilities were enhanced by hardening with glutaraldehyde and hexamethylenediamine.</description><subject>Amidohydrolases - metabolism</subject><subject>Amino Acids</subject><subject>Aspartate Ammonia-Lyase - metabolism</subject><subject>Bacteria - enzymology</subject><subject>Brevibacterium - enzymology</subject><subject>Carrageenan</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Escherichia coli - enzymology</subject><subject>Fumarate Hydratase - metabolism</subject><subject>Membranes, Artificial</subject><subject>Streptomyces - enzymology</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UE1PAjEUbIxfiB69eejJ2-LbdtvuHpUokgDGBKO3pltaUt0PbJcI_HqXQIgnTy_vzbzJzCB0HUMvBiB3uWt6hAOJt9sR6sSQiQhIBseoAwA8oiwj5-gihM92FSnnZ-iUpgwE76DhsCzr3BVuoxpXV7i22FSbdWkCVtUMl077FlYF1qYoAl4GV82xVt6ruTGVqrAKuFSNd6tLdGJVEczVfnbR29PjtP8cjV4Gw_79KNKJYCTKRKKtJsAtNTrllFqtNAeaZiaZ6bwNkgBJLIBlSaoyZll7ozlRs0zkoBntotud7sLX30sTGlm6sHWnKlMvgxSJIEzQLTHaEdsIIXhj5cK7Uvm1jEFuu5Jtc_LQXMu_2Qsv89LMDuxdVS0sdvCPK8z6fy35MJz-Fd4bcaExq8On8l-SCyqYfJ8M5ECMx9PJ4EO-0l92koiw</recordid><startdate>197910</startdate><enddate>197910</enddate><creator>Tosa, Tetsuya</creator><creator>Sato, Tadashi</creator><creator>Mori, Takao</creator><creator>Yamamoto, Kazo</creator><creator>Takata, Isao</creator><creator>Nishida, Yutaka</creator><creator>Chibata, Ichiro</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197910</creationdate><title>Immobilization of enzymes and microbial cells using carrageenan as matrix</title><author>Tosa, Tetsuya ; Sato, Tadashi ; Mori, Takao ; Yamamoto, Kazo ; Takata, Isao ; Nishida, Yutaka ; Chibata, Ichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4752-974cfc206f3ec8633fcac60389e4dcb2604024f00f548a95f5b263b2ad97b0c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Amidohydrolases - metabolism</topic><topic>Amino Acids</topic><topic>Aspartate Ammonia-Lyase - metabolism</topic><topic>Bacteria - enzymology</topic><topic>Brevibacterium - enzymology</topic><topic>Carrageenan</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Escherichia coli - enzymology</topic><topic>Fumarate Hydratase - metabolism</topic><topic>Membranes, Artificial</topic><topic>Streptomyces - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tosa, Tetsuya</creatorcontrib><creatorcontrib>Sato, Tadashi</creatorcontrib><creatorcontrib>Mori, Takao</creatorcontrib><creatorcontrib>Yamamoto, Kazo</creatorcontrib><creatorcontrib>Takata, Isao</creatorcontrib><creatorcontrib>Nishida, Yutaka</creatorcontrib><creatorcontrib>Chibata, Ichiro</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tosa, Tetsuya</au><au>Sato, Tadashi</au><au>Mori, Takao</au><au>Yamamoto, Kazo</au><au>Takata, Isao</au><au>Nishida, Yutaka</au><au>Chibata, Ichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of enzymes and microbial cells using carrageenan as matrix</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>1979-10</date><risdate>1979</risdate><volume>21</volume><issue>10</issue><spage>1697</spage><epage>1709</epage><pages>1697-1709</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><abstract>Conditions for the gelation of k ‐carrageenan, which is a new polymer for immobilization of enzymes and microbial cells, were investigated in detail. k ‐Carrageenan was easily induced to gel by contact with metal ions, amines, amino acid derivatives, and water‐miscible organic solvents. By using this property of k ‐carrageenan, the immobilization of enzymes and microbial cells was investigated. Several kinds of enzymes and microbial cells were easily immobilized with high enzyme activities. Immobilized preparations were easily tailor‐made to various shapes such as cube, bead, and membrane. The obtained immobilized preparations were stable, and columns packed with them were used for continuous enzyme reaction for a long period. Their operational stabilities were enhanced by hardening with glutaraldehyde and hexamethylenediamine.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>385076</pmid><doi>10.1002/bit.260211002</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biotechnology and bioengineering, 1979-10, Vol.21 (10), p.1697-1709 |
| issn | 0006-3592 1097-0290 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amidohydrolases - metabolism Amino Acids Aspartate Ammonia-Lyase - metabolism Bacteria - enzymology Brevibacterium - enzymology Carrageenan Enzymes, Immobilized - metabolism Escherichia coli - enzymology Fumarate Hydratase - metabolism Membranes, Artificial Streptomyces - enzymology |
| title | Immobilization of enzymes and microbial cells using carrageenan as matrix |
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