Isolation of intracisternal type A-particles and associated high-molecular-weight RNA after cell disruption by nitrogen cavitation

The liberation of intracisternal A-type particles from the endoplasmic reticulum of mouse plasmacytoma cells (line MPC-11) without the use of detergents, has been achieved by the technique of nitrogen cavitation. By this method cell rupture is due to a sudden decompression of a cell suspension after...

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Veröffentlicht in:	Analytical biochemistry 1979-08, Vol.97 (1), p.85-94
Hauptverfasser:	Nissen-Meyer, J., Åbro, A., Eikhom, T.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Fractionation - methods Cell Membrane - analysis Endoplasmic Reticulum - analysis Inclusion Bodies, Viral - analysis Inclusion Bodies, Viral - ultrastructure Mice Molecular Weight Nitrogen RNA - isolation & purification
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