Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bovine serum albumin oligomers produced by lipid peroxidation
The oligomers of bovine serum albumin were produced by controlled reaction with peroxidizing linoleic acid to examine their possible utility as calibration proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The polymerization was effected in reaction mixtures containing linoleic...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 1979-07, Vol.86 (1), p.177-182 |
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| container_issue | 1 |
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| container_title | Journal of biochemistry (Tokyo) |
| container_volume | 86 |
| creator | JANADO, Masanobu YANO, Yuki NAKAMORI, Hiroko NISHIDA, Toshiro |
| description | The oligomers of bovine serum albumin were produced by controlled reaction with peroxidizing linoleic acid to examine their possible utility as calibration proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The polymerization was effected in reaction mixtures containing linoleic acid undergoing peroxidation in the presence of ascorbic acid, and conditions that yield soluble oligomers with a wide molecular weight distribution were established. The interaction of these soluble oligomers with sodium dodecyl sulfate exhibited a binding isotherm indistinguishable from that obtained with bovine serum albumin. Furthermore, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the albumin oligomers conformed to the empirical relation of molecular weight to mobility that pertains to the use of these oligomers as standard molecular weight markers. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a132500 |
| format | Article |
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Furthermore, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the albumin oligomers conformed to the empirical relation of molecular weight to mobility that pertains to the use of these oligomers as standard molecular weight markers.</description><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Linoleic Acids</subject><subject>Macromolecular Substances</subject><subject>Molecular Weight</subject><subject>Peroxides</subject><subject>Serum Albumin, Bovine</subject><subject>Sodium Dodecyl Sulfate</subject><issn>0021-924X</issn><issn>1756-2651</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkcuO0zAUhiPErQy8AULewC7FdzcLFlABA1QCcZFGbCzHPu64OHGwE9Ty9GSUaiRWR0f_f27fqarnBK8JbtjLdPQpu0Oacm9iWR9aew3d2hBGBcZ3qhVRQtZUCnK3WmFMSd1QfvWwelTK4SaljD2o7nPVEEpW1d9vyYWpQy45sKeIyhS9GaEeUjwZm0_RdMEB2kNEEMGOOQ3XKUMJBSWP2vQn9IAK5LmFie3UhR6lGPapg1zQkJObLDjUnlAMQ3BogJyOwZkxpP5xdc_PB8CTc7yofrx7-317We8-v_-wfb2rLePNWLdebChIzAXj2DtJcWOYAkwaL3krqWlIszHYGmoEFlYKLj1z1mPGHW-IZRfVi6XvvM7vCcqou1AsxGh6SFPRiisspRCz8dVitDmVksHrIYfO5JMmWN-g1_-j1wt6fUY_1z89D5raDtxt9cJ6lutFDmWE461q8i8tFVNCX1791FvOvn56o3aazv5ni9-bpM0-h6I_fplvxZgrtZnf-A8UX6Lr</recordid><startdate>197907</startdate><enddate>197907</enddate><creator>JANADO, Masanobu</creator><creator>YANO, Yuki</creator><creator>NAKAMORI, Hiroko</creator><creator>NISHIDA, Toshiro</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197907</creationdate><title>Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bovine serum albumin oligomers produced by lipid peroxidation</title><author>JANADO, Masanobu ; YANO, Yuki ; NAKAMORI, Hiroko ; NISHIDA, Toshiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-bf582e6045340fd6209a37e019f64b62a9198a0ca2a505c6546f3dcf034d491c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Linoleic Acids</topic><topic>Macromolecular Substances</topic><topic>Molecular Weight</topic><topic>Peroxides</topic><topic>Serum Albumin, Bovine</topic><topic>Sodium Dodecyl Sulfate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JANADO, Masanobu</creatorcontrib><creatorcontrib>YANO, Yuki</creatorcontrib><creatorcontrib>NAKAMORI, Hiroko</creatorcontrib><creatorcontrib>NISHIDA, Toshiro</creatorcontrib><creatorcontrib>Kyoto Women' s Univ. 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The polymerization was effected in reaction mixtures containing linoleic acid undergoing peroxidation in the presence of ascorbic acid, and conditions that yield soluble oligomers with a wide molecular weight distribution were established. The interaction of these soluble oligomers with sodium dodecyl sulfate exhibited a binding isotherm indistinguishable from that obtained with bovine serum albumin. Furthermore, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the albumin oligomers conformed to the empirical relation of molecular weight to mobility that pertains to the use of these oligomers as standard molecular weight markers.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>479121</pmid><doi>10.1093/oxfordjournals.jbchem.a132500</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0021-924X |
| ispartof | Journal of biochemistry (Tokyo), 1979-07, Vol.86 (1), p.177-182 |
| issn | 0021-924X 1756-2651 1756-2651 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74706655 |
| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Free Full-Text Journals in Chemistry; Oxford University Press Journals Digital Archive Legacy |
| subjects | Electrophoresis, Polyacrylamide Gel Linoleic Acids Macromolecular Substances Molecular Weight Peroxides Serum Albumin, Bovine Sodium Dodecyl Sulfate |
| title | Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bovine serum albumin oligomers produced by lipid peroxidation |
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