Rapid Magnetic Purification of Rosette-Forming Lymphocytes
High gradient magnetic separation, which as previously been shown effective in extracting erythrocytes from a flowing cell suspension, has been used to separate rosetted and unrosetted human peripheral blood lymphocytes. The hemoglobin in the sheep red cells used to form rosettes was first oxidizied...
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| Veröffentlicht in: | The Journal of immunology (1950) 1979-10, Vol.123 (4), p.1778-1780 |
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| container_end_page | 1780 |
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| container_issue | 4 |
| container_start_page | 1778 |
| container_title | The Journal of immunology (1950) |
| container_volume | 123 |
| creator | Owen, Charles S Winger, Larry A Symington, Frank W Nowell, Peter C |
| description | High gradient magnetic separation, which as previously been shown effective in extracting erythrocytes from a flowing cell suspension, has been used to separate rosetted and unrosetted human peripheral blood lymphocytes. The hemoglobin in the sheep red cells used to form rosettes was first oxidizied to the paramagnetic methemoglobin form. Samples of 50 x 10(6) lymphocytes could be processed in 10 min under sterile conditions with greater than 90% purity of the rosetted cell fraction and maintenance of T cell function in mixed lymphocyte cultures. |
| doi_str_mv | 10.4049/jimmunol.123.4.1778 |
| format | Article |
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The hemoglobin in the sheep red cells used to form rosettes was first oxidizied to the paramagnetic methemoglobin form. 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The hemoglobin in the sheep red cells used to form rosettes was first oxidizied to the paramagnetic methemoglobin form. Samples of 50 x 10(6) lymphocytes could be processed in 10 min under sterile conditions with greater than 90% purity of the rosetted cell fraction and maintenance of T cell function in mixed lymphocyte cultures.</description><subject>Animals</subject><subject>Cell Separation</subject><subject>Cell Survival</subject><subject>Humans</subject><subject>Lymphocytes - classification</subject><subject>Magnetics</subject><subject>Rosette Formation</subject><subject>Sheep</subject><subject>Time Factors</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkDtPwzAYRS3EqxR-AQyZYEr4_IidsKGKAlIRqILZchO7dZXEIU4U9d-TKgUx3eHee4aD0DWGiAFL77e2LLvKFREmNGIRFiI5QhMcxxByDvwYTQAICbHg4hxdeL8FAA6EnaFTJtI4TSboYalqmwdval3p1mbBR9dYYzPVWlcFzgRL53Xb6nDumtJW62CxK-uNy3at9pfoxKjC66tDTtHX_Olz9hIu3p9fZ4-LMKOCt6EWRnGcMANZgoGA4kyAorBKScyzmMQ6IVhzbBJsTCpUngMYSulKJTw2NKFTdDty68Z9d9q3srQ-00WhKu06LwXjaUo4HYZ0HGaN877RRtaNLVWzkxjkXpj8FSYHYZLJvbDhdXPAd6tS53-f0dBQ3431xq43vW209KUqimGMZd_3_0A_HwV15Q</recordid><startdate>197910</startdate><enddate>197910</enddate><creator>Owen, Charles S</creator><creator>Winger, Larry A</creator><creator>Symington, Frank W</creator><creator>Nowell, Peter C</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197910</creationdate><title>Rapid Magnetic Purification of Rosette-Forming Lymphocytes</title><author>Owen, Charles S ; Winger, Larry A ; Symington, Frank W ; Nowell, Peter C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-e7fa6184f0c81020a6470a30b9256c525e821e61f81ff97add00f333ba865f383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Cell Separation</topic><topic>Cell Survival</topic><topic>Humans</topic><topic>Lymphocytes - classification</topic><topic>Magnetics</topic><topic>Rosette Formation</topic><topic>Sheep</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Owen, Charles S</creatorcontrib><creatorcontrib>Winger, Larry A</creatorcontrib><creatorcontrib>Symington, Frank W</creatorcontrib><creatorcontrib>Nowell, Peter C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Owen, Charles S</au><au>Winger, Larry A</au><au>Symington, Frank W</au><au>Nowell, Peter C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Magnetic Purification of Rosette-Forming Lymphocytes</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1979-10</date><risdate>1979</risdate><volume>123</volume><issue>4</issue><spage>1778</spage><epage>1780</epage><pages>1778-1780</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>High gradient magnetic separation, which as previously been shown effective in extracting erythrocytes from a flowing cell suspension, has been used to separate rosetted and unrosetted human peripheral blood lymphocytes. 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| fulltext | fulltext |
| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1979-10, Vol.123 (4), p.1778-1780 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74699263 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Separation Cell Survival Humans Lymphocytes - classification Magnetics Rosette Formation Sheep Time Factors |
| title | Rapid Magnetic Purification of Rosette-Forming Lymphocytes |
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