Improved Spectrophotometric Determination of Glycerol and Its Comparison with an Enzymatic Method
A chemical method for the determination of glycerol was developed and compared to an enzymatic assay for sensitivity and reproducibility. The chemical assay is based on glycerol oxidation to formaldehyde and subsequent reaction with chromotropic acid to yield a colored product. With this method, as...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 1979-08, Vol.68 (8), p.1064-1066 |
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| container_end_page | 1066 |
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| container_issue | 8 |
| container_start_page | 1064 |
| container_title | Journal of pharmaceutical sciences |
| container_volume | 68 |
| creator | Sturgeon, Roy J. Deamer, Robert L. Harbison, Harry A. |
| description | A chemical method for the determination of glycerol was developed and compared to an enzymatic assay for sensitivity and reproducibility. The chemical assay is based on glycerol oxidation to formaldehyde and subsequent reaction with chromotropic acid to yield a colored product. With this method, as little as 5 μg of glycerol/ml can be detected. The enzymatic assay is based on enzymatic glycerol phosphorylation followed by glycerol phosphate dehydrogenation by nadide (nicotinamide adenine dinucleotide). The reduced nadide is used to reduce iodonitrotetrazolium violet to its colored formazan product. The enzymatic method can be used to determine 50 μg of glycerol/ml in aqueous samples. |
| doi_str_mv | 10.1002/jps.2600680841 |
| format | Article |
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The chemical assay is based on glycerol oxidation to formaldehyde and subsequent reaction with chromotropic acid to yield a colored product. With this method, as little as 5 μg of glycerol/ml can be detected. The enzymatic assay is based on enzymatic glycerol phosphorylation followed by glycerol phosphate dehydrogenation by nadide (nicotinamide adenine dinucleotide). The reduced nadide is used to reduce iodonitrotetrazolium violet to its colored formazan product. The enzymatic method can be used to determine 50 μg of glycerol/ml in aqueous samples.</description><identifier>ISSN: 0022-3549</identifier><identifier>EISSN: 1520-6017</identifier><identifier>DOI: 10.1002/jps.2600680841</identifier><identifier>PMID: 480166</identifier><language>eng</language><publisher>Washington: Elsevier Inc</publisher><subject>comparison with enzymatic method ; glycerol ; Glycerol - analysis ; Glycerolphosphate Dehydrogenase - analysis ; Glycerol—analysis, spectrophotometry, comparison with enzymatic method ; Indicators and Reagents ; spectrophotometry ; Spectrophotometry - methods ; Spectrophotometry-analysis ; Spectrophotometry—analysis, glycerol, comparison with enzymatic method</subject><ispartof>Journal of pharmaceutical sciences, 1979-08, Vol.68 (8), p.1064-1066</ispartof><rights>1979 Wiley-Liss, Inc., A Wiley Company</rights><rights>Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4261-2e22917a4ff0262e6fc83ac3c56406becfd73b2116b3cbd3bb4a5c39d206336f3</citedby><cites>FETCH-LOGICAL-c4261-2e22917a4ff0262e6fc83ac3c56406becfd73b2116b3cbd3bb4a5c39d206336f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjps.2600680841$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjps.2600680841$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/480166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sturgeon, Roy J.</creatorcontrib><creatorcontrib>Deamer, Robert L.</creatorcontrib><creatorcontrib>Harbison, Harry A.</creatorcontrib><title>Improved Spectrophotometric Determination of Glycerol and Its Comparison with an Enzymatic Method</title><title>Journal of pharmaceutical sciences</title><addtitle>J. Pharm. Sci</addtitle><description>A chemical method for the determination of glycerol was developed and compared to an enzymatic assay for sensitivity and reproducibility. The chemical assay is based on glycerol oxidation to formaldehyde and subsequent reaction with chromotropic acid to yield a colored product. With this method, as little as 5 μg of glycerol/ml can be detected. The enzymatic assay is based on enzymatic glycerol phosphorylation followed by glycerol phosphate dehydrogenation by nadide (nicotinamide adenine dinucleotide). The reduced nadide is used to reduce iodonitrotetrazolium violet to its colored formazan product. The enzymatic method can be used to determine 50 μg of glycerol/ml in aqueous samples.</description><subject>comparison with enzymatic method</subject><subject>glycerol</subject><subject>Glycerol - analysis</subject><subject>Glycerolphosphate Dehydrogenase - analysis</subject><subject>Glycerol—analysis, spectrophotometry, comparison with enzymatic method</subject><subject>Indicators and Reagents</subject><subject>spectrophotometry</subject><subject>Spectrophotometry - methods</subject><subject>Spectrophotometry-analysis</subject><subject>Spectrophotometry—analysis, glycerol, comparison with enzymatic method</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1P3DAQxS1UoFvKlVMPPvWWrT8SJzlWW1i2BVqJVhwtx55oDUmc2l7o8tfjKgjEAXEaad7vPWneIHREyZwSwr5cj2HOBCGiIlVOd9CMFoxkgtDyHZolgGW8yOv36EMI1yRhpCj20V5eESrEDKlVP3p3CwZfjqCjd-PaRddD9FbjbxDB93ZQ0boBuxYvu60G7zqsBoNXMeCF60flbUjynY3rtMfHw_22Tw6NzyGunfmIdlvVBTh8nAfoz8nx78VpdvZzuVp8Pct0zgTNGDBW01LlbUuYYCBaXXGluS5ETkQDujUlbxilouG6MbxpclVoXhtGBOei5Qfo85Sb7vm7gRBlb4OGrlMDuE2QZS7Kmtc8gfMJ1N6F4KGVo7e98ltJifxfqUyVyudKk-HTY_Km6cE84VOHSa4n-c52sH0jTH7_dfkiOpu8NkT49-RV_kaKkpeFvLpYSn5SnV7VP6g8T3w18ZCavLXgZdAWBg3G-vQ-aZx97YoHIcyllw</recordid><startdate>197908</startdate><enddate>197908</enddate><creator>Sturgeon, Roy J.</creator><creator>Deamer, Robert L.</creator><creator>Harbison, Harry A.</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197908</creationdate><title>Improved Spectrophotometric Determination of Glycerol and Its Comparison with an Enzymatic Method</title><author>Sturgeon, Roy J. ; Deamer, Robert L. ; Harbison, Harry A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4261-2e22917a4ff0262e6fc83ac3c56406becfd73b2116b3cbd3bb4a5c39d206336f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>comparison with enzymatic method</topic><topic>glycerol</topic><topic>Glycerol - analysis</topic><topic>Glycerolphosphate Dehydrogenase - analysis</topic><topic>Glycerol—analysis, spectrophotometry, comparison with enzymatic method</topic><topic>Indicators and Reagents</topic><topic>spectrophotometry</topic><topic>Spectrophotometry - methods</topic><topic>Spectrophotometry-analysis</topic><topic>Spectrophotometry—analysis, glycerol, comparison with enzymatic method</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sturgeon, Roy J.</creatorcontrib><creatorcontrib>Deamer, Robert L.</creatorcontrib><creatorcontrib>Harbison, Harry A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sturgeon, Roy J.</au><au>Deamer, Robert L.</au><au>Harbison, Harry A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved Spectrophotometric Determination of Glycerol and Its Comparison with an Enzymatic Method</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>1979-08</date><risdate>1979</risdate><volume>68</volume><issue>8</issue><spage>1064</spage><epage>1066</epage><pages>1064-1066</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><abstract>A chemical method for the determination of glycerol was developed and compared to an enzymatic assay for sensitivity and reproducibility. The chemical assay is based on glycerol oxidation to formaldehyde and subsequent reaction with chromotropic acid to yield a colored product. With this method, as little as 5 μg of glycerol/ml can be detected. The enzymatic assay is based on enzymatic glycerol phosphorylation followed by glycerol phosphate dehydrogenation by nadide (nicotinamide adenine dinucleotide). The reduced nadide is used to reduce iodonitrotetrazolium violet to its colored formazan product. The enzymatic method can be used to determine 50 μg of glycerol/ml in aqueous samples.</abstract><cop>Washington</cop><pub>Elsevier Inc</pub><pmid>480166</pmid><doi>10.1002/jps.2600680841</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3549 |
| ispartof | Journal of pharmaceutical sciences, 1979-08, Vol.68 (8), p.1064-1066 |
| issn | 0022-3549 1520-6017 |
| language | eng |
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| source | MEDLINE; Wiley Journals; Alma/SFX Local Collection |
| subjects | comparison with enzymatic method glycerol Glycerol - analysis Glycerolphosphate Dehydrogenase - analysis Glycerol—analysis, spectrophotometry, comparison with enzymatic method Indicators and Reagents spectrophotometry Spectrophotometry - methods Spectrophotometry-analysis Spectrophotometry—analysis, glycerol, comparison with enzymatic method |
| title | Improved Spectrophotometric Determination of Glycerol and Its Comparison with an Enzymatic Method |
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