Determination of aldoses and uronic acid content of vegetable fiber

The factors affecting the stability, hydrolysis, reduction, acetylation, quantitation, and identification of the neutral sugars from vegetable fiber preparations have been studied critically and optimized. The recommended method offers a consolidation of the recent modifications of the alditol aceta...

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Veröffentlicht in:Analytical biochemistry 1979-07, Vol.96 (2), p.282-292
Hauptverfasser: Selvendran, Robert R., March, John F., Ring, Stephen G.
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creator Selvendran, Robert R.
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description The factors affecting the stability, hydrolysis, reduction, acetylation, quantitation, and identification of the neutral sugars from vegetable fiber preparations have been studied critically and optimized. The recommended method offers a consolidation of the recent modifications of the alditol acetate procedure for the estimation of neutral sugars. The recovery of the sugars was tested by glc and ion-exchange chromatography. Also, the modified carbazole method of Bitter and Muir was adapted to make it applicable for the estimation of uronic acid content of fiber because uronic acid cannot be estimated quantitatively by the acetylation procedure. It is emphasized that the proposed method is applicable only to highly purified fiber preparations which are free of coprecipitated intracellular compounds. Also, the levels of pentoses and hexoses in the fiber must be well defined and a suitable correction made for their interference in the assay.
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subjects Carbohydrates - analysis
Cell Wall - analysis
Chromatography, Gas
Chromatography, Ion Exchange
Dietary Fiber
Hydrolysis
Methods
Plants
Space life sciences
Uronic Acids - analysis
Vegetables - analysis
title Determination of aldoses and uronic acid content of vegetable fiber
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