Determination of aldoses and uronic acid content of vegetable fiber
The factors affecting the stability, hydrolysis, reduction, acetylation, quantitation, and identification of the neutral sugars from vegetable fiber preparations have been studied critically and optimized. The recommended method offers a consolidation of the recent modifications of the alditol aceta...
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Veröffentlicht in: | Analytical biochemistry 1979-07, Vol.96 (2), p.282-292 |
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creator | Selvendran, Robert R. March, John F. Ring, Stephen G. |
description | The factors affecting the stability, hydrolysis, reduction, acetylation, quantitation, and identification of the neutral sugars from vegetable fiber preparations have been studied critically and optimized. The recommended method offers a consolidation of the recent modifications of the alditol acetate procedure for the estimation of neutral sugars. The recovery of the sugars was tested by glc and ion-exchange chromatography. Also, the modified carbazole method of Bitter and Muir was adapted to make it applicable for the estimation of uronic acid content of fiber because uronic acid cannot be estimated quantitatively by the acetylation procedure. It is emphasized that the proposed method is applicable only to highly purified fiber preparations which are free of coprecipitated intracellular compounds. Also, the levels of pentoses and hexoses in the fiber must be well defined and a suitable correction made for their interference in the assay. |
doi_str_mv | 10.1016/0003-2697(79)90583-9 |
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Programa Academico de Graduados</creatorcontrib><description>The factors affecting the stability, hydrolysis, reduction, acetylation, quantitation, and identification of the neutral sugars from vegetable fiber preparations have been studied critically and optimized. The recommended method offers a consolidation of the recent modifications of the alditol acetate procedure for the estimation of neutral sugars. The recovery of the sugars was tested by glc and ion-exchange chromatography. Also, the modified carbazole method of Bitter and Muir was adapted to make it applicable for the estimation of uronic acid content of fiber because uronic acid cannot be estimated quantitatively by the acetylation procedure. It is emphasized that the proposed method is applicable only to highly purified fiber preparations which are free of coprecipitated intracellular compounds. Also, the levels of pentoses and hexoses in the fiber must be well defined and a suitable correction made for their interference in the assay.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(79)90583-9</identifier><identifier>PMID: 474957</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Carbohydrates - analysis ; Cell Wall - analysis ; Chromatography, Gas ; Chromatography, Ion Exchange ; Dietary Fiber ; Hydrolysis ; Methods ; Plants ; Space life sciences ; Uronic Acids - analysis ; Vegetables - analysis</subject><ispartof>Analytical biochemistry, 1979-07, Vol.96 (2), p.282-292</ispartof><rights>1979</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-3296c9dc438740354c6c31780be12edcf89829fada22de4251c3958e93f5da1b3</citedby><cites>FETCH-LOGICAL-c445t-3296c9dc438740354c6c31780be12edcf89829fada22de4251c3958e93f5da1b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269779905839$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/474957$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Selvendran, Robert R.</creatorcontrib><creatorcontrib>March, John F.</creatorcontrib><creatorcontrib>Ring, Stephen G.</creatorcontrib><creatorcontrib>Universidad Nacional Agraria La Molina, (Peru). 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It is emphasized that the proposed method is applicable only to highly purified fiber preparations which are free of coprecipitated intracellular compounds. Also, the levels of pentoses and hexoses in the fiber must be well defined and a suitable correction made for their interference in the assay.</description><subject>Carbohydrates - analysis</subject><subject>Cell Wall - analysis</subject><subject>Chromatography, Gas</subject><subject>Chromatography, Ion Exchange</subject><subject>Dietary Fiber</subject><subject>Hydrolysis</subject><subject>Methods</subject><subject>Plants</subject><subject>Space life sciences</subject><subject>Uronic Acids - analysis</subject><subject>Vegetables - analysis</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYP4Gh__YJCuRBfVPJtkI8j4BMGFug5pcjtEOs2YdAb893aszNLVXZzvHC4fQlOCrwgm1TXGmJW00vJC6kuNhWKl3kETgnVVYob1LppskUN0lPMnxoRwUR2gfS65FnKCZnfQQ1qEzvYhdkVsCtv6mCEXtvPFKsUuuMK64AsXux66foOsYQ69rVsomlBDOkF7jW0znP7dY_TxcP8-eypfXh-fZ7cvpeNc9CWjunLaO86U5JgJ7irHiFS4BkLBu0ZpRXVjvaXUA6eCOKaFAs0a4S2p2TE6H3eXKX6tIPdmEbKDtrUdxFU2kldSSSoHkI-gSzHnBI1ZprCw6dsQbDbqzMaL2XgxUptfdUYPtenf_qpegN-WRldDfDbGjY3GzlPI5uONaIWxoIpXagBuRgAGCesAyWQXoHPgQwLXGx_D_w_8AMnoha0</recordid><startdate>19790715</startdate><enddate>19790715</enddate><creator>Selvendran, Robert R.</creator><creator>March, John F.</creator><creator>Ring, Stephen G.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19790715</creationdate><title>Determination of aldoses and uronic acid content of vegetable fiber</title><author>Selvendran, Robert R. ; March, John F. ; Ring, Stephen G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-3296c9dc438740354c6c31780be12edcf89829fada22de4251c3958e93f5da1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Carbohydrates - analysis</topic><topic>Cell Wall - analysis</topic><topic>Chromatography, Gas</topic><topic>Chromatography, Ion Exchange</topic><topic>Dietary Fiber</topic><topic>Hydrolysis</topic><topic>Methods</topic><topic>Plants</topic><topic>Space life sciences</topic><topic>Uronic Acids - analysis</topic><topic>Vegetables - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Selvendran, Robert R.</creatorcontrib><creatorcontrib>March, John F.</creatorcontrib><creatorcontrib>Ring, Stephen G.</creatorcontrib><creatorcontrib>Universidad Nacional Agraria La Molina, (Peru). 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Also, the modified carbazole method of Bitter and Muir was adapted to make it applicable for the estimation of uronic acid content of fiber because uronic acid cannot be estimated quantitatively by the acetylation procedure. It is emphasized that the proposed method is applicable only to highly purified fiber preparations which are free of coprecipitated intracellular compounds. Also, the levels of pentoses and hexoses in the fiber must be well defined and a suitable correction made for their interference in the assay.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>474957</pmid><doi>10.1016/0003-2697(79)90583-9</doi><tpages>11</tpages></addata></record> |
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subjects | Carbohydrates - analysis Cell Wall - analysis Chromatography, Gas Chromatography, Ion Exchange Dietary Fiber Hydrolysis Methods Plants Space life sciences Uronic Acids - analysis Vegetables - analysis |
title | Determination of aldoses and uronic acid content of vegetable fiber |
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