Immobilization of Penicillium vitale glucose-oxidase on aminosilochrome and properties of immobilized enzyme

Immobilization of Penicillium vitale glucose-oxidase on aminosilochrome and properties of immobilized enzyme

Penicillium vitale glucose-oxidase modified by means of the carbohydrate component oxidation is added covalently to aminoorganosylochrome. The activity of the immobilized preparations is 20-38% depending on the protein-carrier ration in immobilization. Comparison of some properties of native and imm...

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Veröffentlicht in:Weekly epidemiological record 1979-07, Vol.51 (4), p.363-368
Hauptverfasser: Degtiar', R G, Gulyĭ, M F
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creator Degtiar', R G
Gulyĭ, M F
description Penicillium vitale glucose-oxidase modified by means of the carbohydrate component oxidation is added covalently to aminoorganosylochrome. The activity of the immobilized preparations is 20-38% depending on the protein-carrier ration in immobilization. Comparison of some properties of native and immobilized glucose-oxidase showed that the rH optimum of the immobilized glucose-oxidase is slightly widened towards the alkaline regions; the immobilized glucose-oxidase possesses a considerably higher pH-stability at pH alkaline values; the immobilized glucose-oxidase preparations are characterized by a significantly greater thermostability: their thermoinactivation constant at 65 degrees C is 8-10 times lower than that of the native enzyme.
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title Immobilization of Penicillium vitale glucose-oxidase on aminosilochrome and properties of immobilized enzyme
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