DETERMINATION OF SOME MOLECULAR PARAMETERS OF TRYPTOPHAN HYDROXYLASE FROM RAT MIDBRAIN AND MURINE MAST CELL

DETERMINATION OF SOME MOLECULAR PARAMETERS OF TRYPTOPHAN HYDROXYLASE FROM RAT MIDBRAIN AND MURINE MAST CELL

—The molecular parameters of tryptophan hydroxylase (EC 1.14.16.4) from rat midbrain and murine mast cell were determined by gel filtration and sucrose density gradient ultracentrifugation. The enzyme from rat midbrain has a calculated molecular weight of 220, 000, a Stokes radius of 55.6 Å, a frict...

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Veröffentlicht in:Journal of neurochemistry 1979-07, Vol.33 (1), p.15-21
Hauptverfasser: Kuhn, Donald M., Rosenberg, Robert C., Lovenberg, Walter
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Centrifugation, Density Gradient
Male
Mast Cells - enzymology
Mesencephalon - enzymology
Mice
Molecular Weight
Protein Conformation
Rats
Tryptophan Hydroxylase
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creator Kuhn, Donald M.
Rosenberg, Robert C.
Lovenberg, Walter
description —The molecular parameters of tryptophan hydroxylase (EC 1.14.16.4) from rat midbrain and murine mast cell were determined by gel filtration and sucrose density gradient ultracentrifugation. The enzyme from rat midbrain has a calculated molecular weight of 220, 000, a Stokes radius of 55.6 Å, a frictional ratio of 1.28, and a sedimentation coefficient of 9.63 S. The mast cell enzyme has a calculated molecular weight of 144, 000, a Stokes radius of 50.3 Å, a frictional ratio of 1.35, and a sedimentation coefficient of 6.97 S. It appears that the tryptophan hydroxylase species from rat midbrain and murine mast cell represent distinct molecular entities.
doi_str_mv 10.1111/j.1471-4159.1979.tb11700.x
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The enzyme from rat midbrain has a calculated molecular weight of 220, 000, a Stokes radius of 55.6 Å, a frictional ratio of 1.28, and a sedimentation coefficient of 9.63 S. The mast cell enzyme has a calculated molecular weight of 144, 000, a Stokes radius of 50.3 Å, a frictional ratio of 1.35, and a sedimentation coefficient of 6.97 S. 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subjects Animals
Centrifugation, Density Gradient
Male
Mast Cells - enzymology
Mesencephalon - enzymology
Mice
Molecular Weight
Protein Conformation
Rats
Tryptophan Hydroxylase
title DETERMINATION OF SOME MOLECULAR PARAMETERS OF TRYPTOPHAN HYDROXYLASE FROM RAT MIDBRAIN AND MURINE MAST CELL
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