Competitive Elution of Pertussis Hemagglutinin

Summary The hemagglutinating factor of Hemophilus pertussis culture supernatants can apparently be eluted from human erythrocytes by suspending such erythrocytes in a distilled-water extract of frozen and thawed red cells. Following the removal of the H. pertussis factor from agglutinated cells, the...

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Veröffentlicht in:Experimental biology and medicine (Maywood, N.J.) N.J.), 1950-08, Vol.74 (4), p.669-671
Hauptverfasser: Mattman, Lida H., Edsall, Geoffrey
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container_title Experimental biology and medicine (Maywood, N.J.)
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creator Mattman, Lida H.
Edsall, Geoffrey
description Summary The hemagglutinating factor of Hemophilus pertussis culture supernatants can apparently be eluted from human erythrocytes by suspending such erythrocytes in a distilled-water extract of frozen and thawed red cells. Following the removal of the H. pertussis factor from agglutinated cells, these cells can be re-agglutinated by addition of fresh H. pertussis culture supernatant. The factor cannot be eluted by incubation of agglutinated cells at various temperatures, or by suspending the cells in salt-free isotonic glucose solution.
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Following the removal of the H. pertussis factor from agglutinated cells, these cells can be re-agglutinated by addition of fresh H. pertussis culture supernatant. 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ispartof Experimental biology and medicine (Maywood, N.J.), 1950-08, Vol.74 (4), p.669-671
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source MEDLINE; Alma/SFX Local Collection
subjects Agglutinins
Bordetella pertussis
Hemagglutinins
Humans
Old Medline
Whooping Cough
title Competitive Elution of Pertussis Hemagglutinin
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