Mutants of Candida albicans hypersensitive to calcofluor white display susceptibility to antifungal drugs
An increased infection incidence of Candida albicans (most common human fungal pathogen) contributes to the need of further functional genetic studies and development of new antifungal drugs. We developed a method to create mutants of C. albicans using an antisense cDNA library to interfere with gen...
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| Veröffentlicht in: | Folia microbiologica 2010-03, Vol.55 (2), p.159-166 |
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| creator | He, Zhu-Mei Chen, Jing Li, Hong-Zhou Luo, Di-Qing Yang, Wei |
| description | An increased infection incidence of
Candida albicans
(most common human fungal pathogen) contributes to the need of further functional genetic studies and development of new antifungal drugs. We developed a method to create mutants of
C. albicans
using an antisense cDNA library to interfere with gene expression, followed by screening for hypersensitivity to Calcofluor White (CFW) and the antifungal drugs caspofungin and itraconazole. Mutants with these properties have with a high probability defects in cell-wall integrity. Fifty out of 200 transformant colonies analyzed (25 %) showed hypersensitivity to CFW compared with the parental strain
C. albicans
CAI-4. Most of those CFW-hypersensitive mutants further displayed the susceptibility to antifungal drugs itraconazole and caspofungin using microbroth dilution method M27-A and an agar-diffusion test. The mutants obtained through this procedure could provide a potential model for screening antifungal pro-drugs which show weak action when standard
C. albicans
strain is used and may also aid in further identifying genes involved in cell integrity. In addition, we describe the effect of varying several parameters in electroporation transformation, including treatment with lithium acetate, upon the efficiency of transformation in
C. albicans
. |
| doi_str_mv | 10.1007/s12223-010-0024-7 |
| format | Article |
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Candida albicans
(most common human fungal pathogen) contributes to the need of further functional genetic studies and development of new antifungal drugs. We developed a method to create mutants of
C. albicans
using an antisense cDNA library to interfere with gene expression, followed by screening for hypersensitivity to Calcofluor White (CFW) and the antifungal drugs caspofungin and itraconazole. Mutants with these properties have with a high probability defects in cell-wall integrity. Fifty out of 200 transformant colonies analyzed (25 %) showed hypersensitivity to CFW compared with the parental strain
C. albicans
CAI-4. Most of those CFW-hypersensitive mutants further displayed the susceptibility to antifungal drugs itraconazole and caspofungin using microbroth dilution method M27-A and an agar-diffusion test. The mutants obtained through this procedure could provide a potential model for screening antifungal pro-drugs which show weak action when standard
C. albicans
strain is used and may also aid in further identifying genes involved in cell integrity. In addition, we describe the effect of varying several parameters in electroporation transformation, including treatment with lithium acetate, upon the efficiency of transformation in
C. albicans
.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/s12223-010-0024-7</identifier><identifier>PMID: 20490759</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antifungal Agents - pharmacology ; Antimicrobial agents ; Applied Microbiology ; Biomedical and Life Sciences ; Candida albicans ; Candida albicans - drug effects ; Candida albicans - genetics ; Cell Wall - drug effects ; Cell Wall - genetics ; Drug resistance ; Echinocandins - pharmacology ; Environmental Engineering/Biotechnology ; Gene expression ; Hypersensitivity ; Immunology ; Life Sciences ; Lipopeptides ; Lithium ; Microbial Sensitivity Tests ; Microbiology ; Mutants ; Mutation ; Yeast</subject><ispartof>Folia microbiologica, 2010-03, Vol.55 (2), p.159-166</ispartof><rights>Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c354t-f6109c7b2d385fc59d479ac7c32093cef441220dae4167ae2cbb4d54c687b2b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12223-010-0024-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12223-010-0024-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20490759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Zhu-Mei</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Li, Hong-Zhou</creatorcontrib><creatorcontrib>Luo, Di-Qing</creatorcontrib><creatorcontrib>Yang, Wei</creatorcontrib><title>Mutants of Candida albicans hypersensitive to calcofluor white display susceptibility to antifungal drugs</title><title>Folia microbiologica</title><addtitle>Folia Microbiol</addtitle><addtitle>Folia Microbiol (Praha)</addtitle><description>An increased infection incidence of
Candida albicans
(most common human fungal pathogen) contributes to the need of further functional genetic studies and development of new antifungal drugs. We developed a method to create mutants of
C. albicans
using an antisense cDNA library to interfere with gene expression, followed by screening for hypersensitivity to Calcofluor White (CFW) and the antifungal drugs caspofungin and itraconazole. Mutants with these properties have with a high probability defects in cell-wall integrity. Fifty out of 200 transformant colonies analyzed (25 %) showed hypersensitivity to CFW compared with the parental strain
C. albicans
CAI-4. Most of those CFW-hypersensitive mutants further displayed the susceptibility to antifungal drugs itraconazole and caspofungin using microbroth dilution method M27-A and an agar-diffusion test. The mutants obtained through this procedure could provide a potential model for screening antifungal pro-drugs which show weak action when standard
C. albicans
strain is used and may also aid in further identifying genes involved in cell integrity. In addition, we describe the effect of varying several parameters in electroporation transformation, including treatment with lithium acetate, upon the efficiency of transformation in
C. albicans
.</description><subject>Antifungal Agents - pharmacology</subject><subject>Antimicrobial agents</subject><subject>Applied Microbiology</subject><subject>Biomedical and Life Sciences</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Candida albicans - genetics</subject><subject>Cell Wall - drug effects</subject><subject>Cell Wall - genetics</subject><subject>Drug resistance</subject><subject>Echinocandins - pharmacology</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Gene expression</subject><subject>Hypersensitivity</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Lipopeptides</subject><subject>Lithium</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Yeast</subject><issn>0015-5632</issn><issn>1874-9356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1r3DAQhkVpaLbb_oBeiuglJzejL8s-hiVJAym5pGchS_JGQWu7-mjYf18tm7QQKD3NYZ55Z4YHoU8EvhIAeZ4IpZQ1QKABoLyRb9CKdJI3PRPtW7QCIKIRLaOn6H1KjwAtcEbfoVMKvAcp-hXy30vWU054HvFGT9ZbjXUYvNFTwg_7xcXkpuSz_-VwnrHRwcxjKHPETw8-O2x9WoLe41SScUv2gw8-7w9oTfVjmbY6YBvLNn1AJ6MOyX18rmv04-ryfvOtub27vtlc3DaGCZ6bsSXQGzlQyzoxGtFbLnttpGEUembcyHn9Gqx2nLRSO2qGgVvBTdvVoYGwNTo75i5x_llcymrn620h6MnNJSnJWwaEEfF_kjEheNtDJb-8Ih_nEqf6hmKEyo4R1lWIHCET55SiG9US_U7HvSKgDr7U0ZeqvtTBV12wRp-fg8uwc_bPxIugCtAjkGpr2rr4d_O_U38D0r6hLA</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>He, Zhu-Mei</creator><creator>Chen, Jing</creator><creator>Li, Hong-Zhou</creator><creator>Luo, Di-Qing</creator><creator>Yang, Wei</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20100301</creationdate><title>Mutants of Candida albicans hypersensitive to calcofluor white display susceptibility to antifungal drugs</title><author>He, Zhu-Mei ; 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Candida albicans
(most common human fungal pathogen) contributes to the need of further functional genetic studies and development of new antifungal drugs. We developed a method to create mutants of
C. albicans
using an antisense cDNA library to interfere with gene expression, followed by screening for hypersensitivity to Calcofluor White (CFW) and the antifungal drugs caspofungin and itraconazole. Mutants with these properties have with a high probability defects in cell-wall integrity. Fifty out of 200 transformant colonies analyzed (25 %) showed hypersensitivity to CFW compared with the parental strain
C. albicans
CAI-4. Most of those CFW-hypersensitive mutants further displayed the susceptibility to antifungal drugs itraconazole and caspofungin using microbroth dilution method M27-A and an agar-diffusion test. The mutants obtained through this procedure could provide a potential model for screening antifungal pro-drugs which show weak action when standard
C. albicans
strain is used and may also aid in further identifying genes involved in cell integrity. In addition, we describe the effect of varying several parameters in electroporation transformation, including treatment with lithium acetate, upon the efficiency of transformation in
C. albicans
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>20490759</pmid><doi>10.1007/s12223-010-0024-7</doi><tpages>8</tpages></addata></record> |
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| ispartof | Folia microbiologica, 2010-03, Vol.55 (2), p.159-166 |
| issn | 0015-5632 1874-9356 |
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| subjects | Antifungal Agents - pharmacology Antimicrobial agents Applied Microbiology Biomedical and Life Sciences Candida albicans Candida albicans - drug effects Candida albicans - genetics Cell Wall - drug effects Cell Wall - genetics Drug resistance Echinocandins - pharmacology Environmental Engineering/Biotechnology Gene expression Hypersensitivity Immunology Life Sciences Lipopeptides Lithium Microbial Sensitivity Tests Microbiology Mutants Mutation Yeast |
| title | Mutants of Candida albicans hypersensitive to calcofluor white display susceptibility to antifungal drugs |
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