Characterization of a Novel Plantain Asr Gene, MpAsr, that is Regulated in Response to Infection of Fusarium oxysporum f. sp. cubense and Abiotic Stresses
Asr （abscisic acid, stress, ripening induced） genes are typically upregulated by a wide range of factors, including drought, cold, salt, abscisic acid （ABA） and injury; in addition to plant responses to developmental and environmental signals. We isolated an Asr gene, MpAsr, from a suppression subtr...
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| Veröffentlicht in: | Journal of integrative plant biology 2010-03, Vol.52 (3), p.315-323 |
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| creator | Liu, Hai-Yan Dai, Jin-Ran Feng, Dong-Ru Liu, Bing Wang, Hong-Bin Wang, Jin-Fa |
| description | Asr （abscisic acid, stress, ripening induced） genes are typically upregulated by a wide range of factors, including drought, cold, salt, abscisic acid （ABA） and injury; in addition to plant responses to developmental and environmental signals. We isolated an Asr gene, MpAsr, from a suppression subtractive hybridization （SSH） cDNA library of cold induced plantain （Musa paradisiaca） leaves. MpAsr expression was upregulated in Fusarium oxysporum f. sp. cubense infected plantain leaves, peels and roots, suggesting that MpAsr plays a role in plantain pathogen response. In addition, a 581-bp putative promoter region of MpAsr was isolated via genome walking and cis-elements involved in abioUc stress and pathogen-related responses were detected in this same region. Furthermore, the MpAsr promoter demonstrated positive activity and inducibility in tobacco under F. oxysporum f. sp. cubense infection and ABA, cold, dehydration and high salt concentration treatments. Interestingly, transgenic Arabidopsis plants overexpressing MpAsr exhibited higher drought tolerance, but showed no significant decreased sensitivity to F. oxysporum f. sp. cubense. These results suggest that MpAsr might be involved in plant responses to both abiotic stress and pathogen attack. |
| doi_str_mv | 10.1111/j.1744-7909.2010.00912.x |
| format | Article |
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We isolated an Asr gene, MpAsr, from a suppression subtractive hybridization （SSH） cDNA library of cold induced plantain （Musa paradisiaca） leaves. MpAsr expression was upregulated in Fusarium oxysporum f. sp. cubense infected plantain leaves, peels and roots, suggesting that MpAsr plays a role in plantain pathogen response. In addition, a 581-bp putative promoter region of MpAsr was isolated via genome walking and cis-elements involved in abioUc stress and pathogen-related responses were detected in this same region. Furthermore, the MpAsr promoter demonstrated positive activity and inducibility in tobacco under F. oxysporum f. sp. cubense infection and ABA, cold, dehydration and high salt concentration treatments. Interestingly, transgenic Arabidopsis plants overexpressing MpAsr exhibited higher drought tolerance, but showed no significant decreased sensitivity to F. oxysporum f. sp. cubense. These results suggest that MpAsr might be involved in plant responses to both abiotic stress and pathogen attack.</description><identifier>ISSN: 1672-9072</identifier><identifier>EISSN: 1744-7909</identifier><identifier>DOI: 10.1111/j.1744-7909.2010.00912.x</identifier><identifier>PMID: 20377692</identifier><language>eng</language><publisher>Melbourne, Australia: Melbourne, Australia : Blackwell Publishing Asia</publisher><subject>Abscisic acid ; Adaptation, Physiological - genetics ; Amino Acid Sequence ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - microbiology ; Base Sequence ; Dehydration ; Drought resistance ; Droughts ; Fusarium - physiology ; Fusarium oxysporum ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Gene mapping ; Genes, Plant - genetics ; Genomes ; Infection ; Injuries ; Leaves ; Molecular Sequence Data ; Musa paradisiaca ; Pathogens ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plantago - genetics ; Plantago - microbiology ; Plants, Genetically Modified ; Promoter Regions, Genetic - genetics ; Promoters ; Ripening ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Roots ; Salts ; Sequence Analysis, DNA ; Stress ; Stress, Physiological - genetics ; Tobacco ; Transgenic plants ; 基因组 ; 抑制性消减杂交 ; 病菌感染 ; 非生物胁迫</subject><ispartof>Journal of integrative plant biology, 2010-03, Vol.52 (3), p.315-323</ispartof><rights>2010 Institute of Botany, Chinese Academy of Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4892-2a1799ddd01cba67f917357a6ed070134114deebcfc2516bcfdca68ed3d991d03</citedby><cites>FETCH-LOGICAL-c4892-2a1799ddd01cba67f917357a6ed070134114deebcfc2516bcfdca68ed3d991d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/94176A/94176A.jpg</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1744-7909.2010.00912.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1744-7909.2010.00912.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20377692$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Hai-Yan</creatorcontrib><creatorcontrib>Dai, Jin-Ran</creatorcontrib><creatorcontrib>Feng, Dong-Ru</creatorcontrib><creatorcontrib>Liu, Bing</creatorcontrib><creatorcontrib>Wang, Hong-Bin</creatorcontrib><creatorcontrib>Wang, Jin-Fa</creatorcontrib><title>Characterization of a Novel Plantain Asr Gene, MpAsr, that is Regulated in Response to Infection of Fusarium oxysporum f. sp. cubense and Abiotic Stresses</title><title>Journal of integrative plant biology</title><addtitle>Journal of Integrative Plant Biology</addtitle><description>Asr （abscisic acid, stress, ripening induced） genes are typically upregulated by a wide range of factors, including drought, cold, salt, abscisic acid （ABA） and injury; in addition to plant responses to developmental and environmental signals. We isolated an Asr gene, MpAsr, from a suppression subtractive hybridization （SSH） cDNA library of cold induced plantain （Musa paradisiaca） leaves. MpAsr expression was upregulated in Fusarium oxysporum f. sp. cubense infected plantain leaves, peels and roots, suggesting that MpAsr plays a role in plantain pathogen response. In addition, a 581-bp putative promoter region of MpAsr was isolated via genome walking and cis-elements involved in abioUc stress and pathogen-related responses were detected in this same region. Furthermore, the MpAsr promoter demonstrated positive activity and inducibility in tobacco under F. oxysporum f. sp. cubense infection and ABA, cold, dehydration and high salt concentration treatments. Interestingly, transgenic Arabidopsis plants overexpressing MpAsr exhibited higher drought tolerance, but showed no significant decreased sensitivity to F. oxysporum f. sp. cubense. These results suggest that MpAsr might be involved in plant responses to both abiotic stress and pathogen attack.</description><subject>Abscisic acid</subject><subject>Adaptation, Physiological - genetics</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - microbiology</subject><subject>Base Sequence</subject><subject>Dehydration</subject><subject>Drought resistance</subject><subject>Droughts</subject><subject>Fusarium - physiology</subject><subject>Fusarium oxysporum</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene mapping</subject><subject>Genes, Plant - genetics</subject><subject>Genomes</subject><subject>Infection</subject><subject>Injuries</subject><subject>Leaves</subject><subject>Molecular Sequence Data</subject><subject>Musa paradisiaca</subject><subject>Pathogens</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plantago - genetics</subject><subject>Plantago - microbiology</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Promoters</subject><subject>Ripening</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Roots</subject><subject>Salts</subject><subject>Sequence Analysis, DNA</subject><subject>Stress</subject><subject>Stress, Physiological - genetics</subject><subject>Tobacco</subject><subject>Transgenic plants</subject><subject>基因组</subject><subject>抑制性消减杂交</subject><subject>病菌感染</subject><subject>非生物胁迫</subject><issn>1672-9072</issn><issn>1744-7909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1y0zAUhT0MDC2FVwANGza10Y9t2QsWaYaGtKF0Wjqw08jSdaLUsVLJhoRH4WmRSZotaHPvSN85V9KJIkRwQsJ6v0wIT9OYl7hMKA67GJeEJpsn0fHh4Gnoc07jEnN6FL3wfokxK3BOn0dHFDPO85IeR7_HC-mk6sCZX7IztkW2RhJd2R_QoOtGtp00LRp5hybQwin6vA79KeoWskPGoxuY943sQKNA3YBf29YD6iyatjWoR7_z3ktn-hWym21AXOjqBPl1glRfwaCQrUajytjOKHTbOfAe_MvoWS0bD6_29SS6O__4dfwpnn2ZTMejWazSoqQxlYSXpdYaE1XJnNcl4SzjMgeNOSYsJSTVAJWqFc1IHqpWMi9AM12WRGN2Er3b-a6dfejBd2JlvIImPB5s7wVPc8qzMOPfJGNFXtAiC2SxI5Wz3juoxdqZlXRbQbAYIhRLMSQlhqTEEKH4G6HYBOnr_ZC-WoE-CB8zC8CHHfDTNLD9b2NxMb0-C13Qxzu98R1sDnrp7kXOGc_Et6uJmF3y8cXk8ruYBP7Njq-lFXLujBd3t8GZYVJgnpHhX97uX7uw7fzBtHNRSXVfh_sJxhjJs5SxP_ZXzz8</recordid><startdate>201003</startdate><enddate>201003</enddate><creator>Liu, Hai-Yan</creator><creator>Dai, Jin-Ran</creator><creator>Feng, Dong-Ru</creator><creator>Liu, Bing</creator><creator>Wang, Hong-Bin</creator><creator>Wang, Jin-Fa</creator><general>Melbourne, Australia : Blackwell Publishing Asia</general><general>Blackwell Publishing Asia</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>201003</creationdate><title>Characterization of a Novel Plantain Asr Gene, MpAsr, that is Regulated in Response to Infection of Fusarium oxysporum f. sp. cubense and Abiotic Stresses</title><author>Liu, Hai-Yan ; Dai, Jin-Ran ; Feng, Dong-Ru ; Liu, Bing ; Wang, Hong-Bin ; Wang, Jin-Fa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4892-2a1799ddd01cba67f917357a6ed070134114deebcfc2516bcfdca68ed3d991d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Abscisic acid</topic><topic>Adaptation, Physiological - genetics</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - microbiology</topic><topic>Base Sequence</topic><topic>Dehydration</topic><topic>Drought resistance</topic><topic>Droughts</topic><topic>Fusarium - physiology</topic><topic>Fusarium oxysporum</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene mapping</topic><topic>Genes, Plant - genetics</topic><topic>Genomes</topic><topic>Infection</topic><topic>Injuries</topic><topic>Leaves</topic><topic>Molecular Sequence Data</topic><topic>Musa paradisiaca</topic><topic>Pathogens</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plantago - genetics</topic><topic>Plantago - microbiology</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Promoters</topic><topic>Ripening</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Roots</topic><topic>Salts</topic><topic>Sequence Analysis, DNA</topic><topic>Stress</topic><topic>Stress, Physiological - genetics</topic><topic>Tobacco</topic><topic>Transgenic plants</topic><topic>基因组</topic><topic>抑制性消减杂交</topic><topic>病菌感染</topic><topic>非生物胁迫</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Hai-Yan</creatorcontrib><creatorcontrib>Dai, Jin-Ran</creatorcontrib><creatorcontrib>Feng, Dong-Ru</creatorcontrib><creatorcontrib>Liu, Bing</creatorcontrib><creatorcontrib>Wang, Hong-Bin</creatorcontrib><creatorcontrib>Wang, Jin-Fa</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of integrative plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Hai-Yan</au><au>Dai, Jin-Ran</au><au>Feng, Dong-Ru</au><au>Liu, Bing</au><au>Wang, Hong-Bin</au><au>Wang, Jin-Fa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a Novel Plantain Asr Gene, MpAsr, that is Regulated in Response to Infection of Fusarium oxysporum f. sp. cubense and Abiotic Stresses</atitle><jtitle>Journal of integrative plant biology</jtitle><addtitle>Journal of Integrative Plant Biology</addtitle><date>2010-03</date><risdate>2010</risdate><volume>52</volume><issue>3</issue><spage>315</spage><epage>323</epage><pages>315-323</pages><issn>1672-9072</issn><eissn>1744-7909</eissn><abstract>Asr （abscisic acid, stress, ripening induced） genes are typically upregulated by a wide range of factors, including drought, cold, salt, abscisic acid （ABA） and injury; in addition to plant responses to developmental and environmental signals. We isolated an Asr gene, MpAsr, from a suppression subtractive hybridization （SSH） cDNA library of cold induced plantain （Musa paradisiaca） leaves. MpAsr expression was upregulated in Fusarium oxysporum f. sp. cubense infected plantain leaves, peels and roots, suggesting that MpAsr plays a role in plantain pathogen response. In addition, a 581-bp putative promoter region of MpAsr was isolated via genome walking and cis-elements involved in abioUc stress and pathogen-related responses were detected in this same region. Furthermore, the MpAsr promoter demonstrated positive activity and inducibility in tobacco under F. oxysporum f. sp. cubense infection and ABA, cold, dehydration and high salt concentration treatments. Interestingly, transgenic Arabidopsis plants overexpressing MpAsr exhibited higher drought tolerance, but showed no significant decreased sensitivity to F. oxysporum f. sp. cubense. These results suggest that MpAsr might be involved in plant responses to both abiotic stress and pathogen attack.</abstract><cop>Melbourne, Australia</cop><pub>Melbourne, Australia : Blackwell Publishing Asia</pub><pmid>20377692</pmid><doi>10.1111/j.1744-7909.2010.00912.x</doi><tpages>9</tpages></addata></record> |
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| subjects | Abscisic acid Adaptation, Physiological - genetics Amino Acid Sequence Arabidopsis Arabidopsis - genetics Arabidopsis - microbiology Base Sequence Dehydration Drought resistance Droughts Fusarium - physiology Fusarium oxysporum Gene Expression Profiling Gene Expression Regulation, Plant Gene mapping Genes, Plant - genetics Genomes Infection Injuries Leaves Molecular Sequence Data Musa paradisiaca Pathogens Plant Diseases - genetics Plant Diseases - microbiology Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plantago - genetics Plantago - microbiology Plants, Genetically Modified Promoter Regions, Genetic - genetics Promoters Ripening RNA, Messenger - genetics RNA, Messenger - metabolism Roots Salts Sequence Analysis, DNA Stress Stress, Physiological - genetics Tobacco Transgenic plants 基因组 抑制性消减杂交 病菌感染 非生物胁迫 |
| title | Characterization of a Novel Plantain Asr Gene, MpAsr, that is Regulated in Response to Infection of Fusarium oxysporum f. sp. cubense and Abiotic Stresses |
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