Immunohistochemical Characterization of Neurones in the Hypoglossal Nucleus of the Pig
With 4 figures and 1 table In this study, the presence of several neurotransmitters and transmitter synthesizing enzymes was studied in hypoglossal nucleus (HN) of the juvenile (4 months old) female pigs (n = 3). Double-labeling immunofluorescence revealed neurones expressing cholinacetyltranspheras...
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| Veröffentlicht in: | Anatomia, histologia, embryologia histologia, embryologia, 2010-04, Vol.39 (2), p.152-159 |
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| creator | Sienkiewicz, W Dudek, A Kaleczyc, J Chrószcz, A |
| description | With 4 figures and 1 table In this study, the presence of several neurotransmitters and transmitter synthesizing enzymes was studied in hypoglossal nucleus (HN) of the juvenile (4 months old) female pigs (n = 3). Double-labeling immunofluorescence revealed neurones expressing cholinacetyltranspherase (ChAT), calcitonin gene-related peptide (CGRP), nitric oxide synthase (NOS), and somatostatin (SOM). Nerve fibers within HN were ChAT, CGRP, NOS, SOM, substance P (SP), Leu-5-enkephalin (Leu-5-Enk), ß-dopamine hydroxylase (DßH), neuropeptide Y (NPY) positive. Virtually all the perikarya contained ChAT, whereas CGRP was present in 47% of the neurones. Nerve cell bodies containing NOS or SOM were only occasionally observed. Immunoreactive nerve fibers were found in a close vicinity of the perikarya, often forming baskets around nerve cell bodies. The results obtained were compared with similar data obtained in other species. The presence of immunoreactive structures, origin of the nerve fibers, and functional significance of the findings are discussed. |
| doi_str_mv | 10.1111/j.1439-0264.2009.00989.x |
| format | Article |
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Double-labeling immunofluorescence revealed neurones expressing cholinacetyltranspherase (ChAT), calcitonin gene-related peptide (CGRP), nitric oxide synthase (NOS), and somatostatin (SOM). Nerve fibers within HN were ChAT, CGRP, NOS, SOM, substance P (SP), Leu-5-enkephalin (Leu-5-Enk), ß-dopamine hydroxylase (DßH), neuropeptide Y (NPY) positive. Virtually all the perikarya contained ChAT, whereas CGRP was present in 47% of the neurones. Nerve cell bodies containing NOS or SOM were only occasionally observed. Immunoreactive nerve fibers were found in a close vicinity of the perikarya, often forming baskets around nerve cell bodies. The results obtained were compared with similar data obtained in other species. The presence of immunoreactive structures, origin of the nerve fibers, and functional significance of the findings are discussed.</description><identifier>ISSN: 0340-2096</identifier><identifier>EISSN: 1439-0264</identifier><identifier>DOI: 10.1111/j.1439-0264.2009.00989.x</identifier><identifier>PMID: 20070290</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animals ; Calcitonin Gene-Related Peptide - analysis ; Calcitonin Gene-Related Peptide - metabolism ; Choline O-Acetyltransferase - analysis ; Choline O-Acetyltransferase - metabolism ; Dopamine beta-Hydroxylase - analysis ; Dopamine beta-Hydroxylase - metabolism ; Enkephalin, Leucine - analysis ; Enkephalin, Leucine - metabolism ; Female ; Hypoglossal Nerve - anatomy & histology ; Immunohistochemistry ; Medulla Oblongata - chemistry ; Medulla Oblongata - cytology ; Medulla Oblongata - metabolism ; Neurons - metabolism ; Neuropeptide Y - analysis ; Neuropeptide Y - metabolism ; Nitric Oxide Synthase - analysis ; Nitric Oxide Synthase - metabolism ; Somatostatin - analysis ; Somatostatin - metabolism ; Substance P - analysis ; Substance P - metabolism ; Swine - anatomy & histology ; Swine - metabolism</subject><ispartof>Anatomia, histologia, embryologia, 2010-04, Vol.39 (2), p.152-159</ispartof><rights>2010 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4619-f5fdfeb4764c7721bf0b5b3fc07d1f7012979a58dd846f67d77a48e304f666853</citedby><cites>FETCH-LOGICAL-c4619-f5fdfeb4764c7721bf0b5b3fc07d1f7012979a58dd846f67d77a48e304f666853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0264.2009.00989.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0264.2009.00989.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20070290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sienkiewicz, W</creatorcontrib><creatorcontrib>Dudek, A</creatorcontrib><creatorcontrib>Kaleczyc, J</creatorcontrib><creatorcontrib>Chrószcz, A</creatorcontrib><title>Immunohistochemical Characterization of Neurones in the Hypoglossal Nucleus of the Pig</title><title>Anatomia, histologia, embryologia</title><addtitle>Anat Histol Embryol</addtitle><description>With 4 figures and 1 table In this study, the presence of several neurotransmitters and transmitter synthesizing enzymes was studied in hypoglossal nucleus (HN) of the juvenile (4 months old) female pigs (n = 3). Double-labeling immunofluorescence revealed neurones expressing cholinacetyltranspherase (ChAT), calcitonin gene-related peptide (CGRP), nitric oxide synthase (NOS), and somatostatin (SOM). Nerve fibers within HN were ChAT, CGRP, NOS, SOM, substance P (SP), Leu-5-enkephalin (Leu-5-Enk), ß-dopamine hydroxylase (DßH), neuropeptide Y (NPY) positive. Virtually all the perikarya contained ChAT, whereas CGRP was present in 47% of the neurones. Nerve cell bodies containing NOS or SOM were only occasionally observed. Immunoreactive nerve fibers were found in a close vicinity of the perikarya, often forming baskets around nerve cell bodies. The results obtained were compared with similar data obtained in other species. The presence of immunoreactive structures, origin of the nerve fibers, and functional significance of the findings are discussed.</description><subject>Animals</subject><subject>Calcitonin Gene-Related Peptide - analysis</subject><subject>Calcitonin Gene-Related Peptide - metabolism</subject><subject>Choline O-Acetyltransferase - analysis</subject><subject>Choline O-Acetyltransferase - metabolism</subject><subject>Dopamine beta-Hydroxylase - analysis</subject><subject>Dopamine beta-Hydroxylase - metabolism</subject><subject>Enkephalin, Leucine - analysis</subject><subject>Enkephalin, Leucine - metabolism</subject><subject>Female</subject><subject>Hypoglossal Nerve - anatomy & histology</subject><subject>Immunohistochemistry</subject><subject>Medulla Oblongata - chemistry</subject><subject>Medulla Oblongata - cytology</subject><subject>Medulla Oblongata - metabolism</subject><subject>Neurons - metabolism</subject><subject>Neuropeptide Y - analysis</subject><subject>Neuropeptide Y - metabolism</subject><subject>Nitric Oxide Synthase - analysis</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Somatostatin - analysis</subject><subject>Somatostatin - metabolism</subject><subject>Substance P - analysis</subject><subject>Substance P - metabolism</subject><subject>Swine - anatomy & histology</subject><subject>Swine - metabolism</subject><issn>0340-2096</issn><issn>1439-0264</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdFu0zAUhi0EYmXwCpA7rhKOY8eOJW6msq0TU0GCbRI3lpPYrUsSFzsRLU-PQ0ZvwZJlS-f7juX_IJRgyHBc73YZpkSkkDOa5QAii7sU2eEJWpwKT9ECCIU0B8HO0IsQdgAME8Gfo7PocMgFLND9TdeNvdvaMLh6qztbqzZZbpVX9aC9_aUG6_rEmWStR-96HRLbJ8NWJ6vj3m1aF0Lk12Pd6jFM2FT6bDcv0TOj2qBfPZ7n6O7q8utyld5-ur5ZXtymNWVYpKYwjdEV5YzWnOe4MlAVFTE18AYbDjgXXKiibJqSMsN4w7mipSZADWOsLMg5ejv33Xv3Y9RhkJ0NtW5b1Ws3BskpyzkhJfybjBSL3NSznMnax-95beTe2075o8Qgp_jlTk4pyyllOcUv_8QvD1F9_fjIWHW6OYl_847A-xn4aVt9_O_G8mJ1GS9RT2c9jksfTrry3yXjhBfyYX0tr_hH_PDh21reR_7NzBvlpNp4G-TdlxwwAVziOABCfgPdiquC</recordid><startdate>201004</startdate><enddate>201004</enddate><creator>Sienkiewicz, W</creator><creator>Dudek, A</creator><creator>Kaleczyc, J</creator><creator>Chrószcz, A</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>201004</creationdate><title>Immunohistochemical Characterization of Neurones in the Hypoglossal Nucleus of the Pig</title><author>Sienkiewicz, W ; Dudek, A ; Kaleczyc, J ; Chrószcz, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4619-f5fdfeb4764c7721bf0b5b3fc07d1f7012979a58dd846f67d77a48e304f666853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Calcitonin Gene-Related Peptide - analysis</topic><topic>Calcitonin Gene-Related Peptide - metabolism</topic><topic>Choline O-Acetyltransferase - analysis</topic><topic>Choline O-Acetyltransferase - metabolism</topic><topic>Dopamine beta-Hydroxylase - analysis</topic><topic>Dopamine beta-Hydroxylase - metabolism</topic><topic>Enkephalin, Leucine - analysis</topic><topic>Enkephalin, Leucine - metabolism</topic><topic>Female</topic><topic>Hypoglossal Nerve - anatomy & histology</topic><topic>Immunohistochemistry</topic><topic>Medulla Oblongata - chemistry</topic><topic>Medulla Oblongata - cytology</topic><topic>Medulla Oblongata - metabolism</topic><topic>Neurons - metabolism</topic><topic>Neuropeptide Y - analysis</topic><topic>Neuropeptide Y - metabolism</topic><topic>Nitric Oxide Synthase - analysis</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Somatostatin - analysis</topic><topic>Somatostatin - metabolism</topic><topic>Substance P - analysis</topic><topic>Substance P - metabolism</topic><topic>Swine - anatomy & histology</topic><topic>Swine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sienkiewicz, W</creatorcontrib><creatorcontrib>Dudek, A</creatorcontrib><creatorcontrib>Kaleczyc, J</creatorcontrib><creatorcontrib>Chrószcz, A</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Anatomia, histologia, embryologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sienkiewicz, W</au><au>Dudek, A</au><au>Kaleczyc, J</au><au>Chrószcz, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical Characterization of Neurones in the Hypoglossal Nucleus of the Pig</atitle><jtitle>Anatomia, histologia, embryologia</jtitle><addtitle>Anat Histol Embryol</addtitle><date>2010-04</date><risdate>2010</risdate><volume>39</volume><issue>2</issue><spage>152</spage><epage>159</epage><pages>152-159</pages><issn>0340-2096</issn><eissn>1439-0264</eissn><abstract>With 4 figures and 1 table In this study, the presence of several neurotransmitters and transmitter synthesizing enzymes was studied in hypoglossal nucleus (HN) of the juvenile (4 months old) female pigs (n = 3). Double-labeling immunofluorescence revealed neurones expressing cholinacetyltranspherase (ChAT), calcitonin gene-related peptide (CGRP), nitric oxide synthase (NOS), and somatostatin (SOM). Nerve fibers within HN were ChAT, CGRP, NOS, SOM, substance P (SP), Leu-5-enkephalin (Leu-5-Enk), ß-dopamine hydroxylase (DßH), neuropeptide Y (NPY) positive. Virtually all the perikarya contained ChAT, whereas CGRP was present in 47% of the neurones. Nerve cell bodies containing NOS or SOM were only occasionally observed. Immunoreactive nerve fibers were found in a close vicinity of the perikarya, often forming baskets around nerve cell bodies. The results obtained were compared with similar data obtained in other species. The presence of immunoreactive structures, origin of the nerve fibers, and functional significance of the findings are discussed.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>20070290</pmid><doi>10.1111/j.1439-0264.2009.00989.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Calcitonin Gene-Related Peptide - analysis Calcitonin Gene-Related Peptide - metabolism Choline O-Acetyltransferase - analysis Choline O-Acetyltransferase - metabolism Dopamine beta-Hydroxylase - analysis Dopamine beta-Hydroxylase - metabolism Enkephalin, Leucine - analysis Enkephalin, Leucine - metabolism Female Hypoglossal Nerve - anatomy & histology Immunohistochemistry Medulla Oblongata - chemistry Medulla Oblongata - cytology Medulla Oblongata - metabolism Neurons - metabolism Neuropeptide Y - analysis Neuropeptide Y - metabolism Nitric Oxide Synthase - analysis Nitric Oxide Synthase - metabolism Somatostatin - analysis Somatostatin - metabolism Substance P - analysis Substance P - metabolism Swine - anatomy & histology Swine - metabolism |
| title | Immunohistochemical Characterization of Neurones in the Hypoglossal Nucleus of the Pig |
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