Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model
Yun JI, Wikesjö UME, Borke JL, Bisch FC, Lewis JE, Herold RW, Swiec GD, Wood JC, McPherson III JC. Effect of systemic parathyroid hormone (1–34) and a β‐tricalcium phosphate biomaterial on local bone formation in a critical‐size rat calvarial defect model. J Clin Periodontol 2010; 37: 419–426 doi: 1...
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| creator | Yun, Jonathan I. Wikesjö, Ulf ME Borke, James L. Bisch, Frederick C. Lewis, Jill E. Herold, Robert W. Swiec, Gary D. Wood, Joseph C. McPherson III, James C. |
| description | Yun JI, Wikesjö UME, Borke JL, Bisch FC, Lewis JE, Herold RW, Swiec GD, Wood JC, McPherson III JC. Effect of systemic parathyroid hormone (1–34) and a β‐tricalcium phosphate biomaterial on local bone formation in a critical‐size rat calvarial defect model. J Clin Periodontol 2010; 37: 419–426 doi: 10.1111/j.1600‐051X.2010.01547.x
Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1–34) (PTH), a surgically implanted synthetic β‐tricalcium phosphate (β‐TCP) bone biomaterial serving as a matrix to support new bone formation.
Materials and Methods: Critical‐size, 8 mm, calvarial through‐and‐through osteotomy defects were surgically created in 100 adult male Sprague–Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β‐TCP, β‐TCP, or particulate human demineralized freeze‐dried bone (DFDB), and sham‐surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post‐surgery for radiographic and histometric analysis.
Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β‐TCP (15.7±2.4%), β‐TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham‐surgery control (10.0±1.5%) at 4 weeks (p |
| doi_str_mv | 10.1111/j.1600-051X.2010.01547.x |
| format | Article |
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Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1–34) (PTH), a surgically implanted synthetic β‐tricalcium phosphate (β‐TCP) bone biomaterial serving as a matrix to support new bone formation.
Materials and Methods: Critical‐size, 8 mm, calvarial through‐and‐through osteotomy defects were surgically created in 100 adult male Sprague–Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β‐TCP, β‐TCP, or particulate human demineralized freeze‐dried bone (DFDB), and sham‐surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post‐surgery for radiographic and histometric analysis.
Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β‐TCP (15.7±2.4%), β‐TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham‐surgery control (10.0±1.5%) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0%) compared with PTH/β‐TCP (22.4±3.0%), β‐TCP (21.3±4.4%), and with the sham‐surgery control (23.8±4.2%) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3%) to 8 weeks (32.0±3.2%) (p<0.006). The PTH/β‐TCP and β‐TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β‐TCP biomaterial.
Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β‐TCP biomaterial.</description><identifier>ISSN: 0303-6979</identifier><identifier>EISSN: 1600-051X</identifier><identifier>DOI: 10.1111/j.1600-051X.2010.01547.x</identifier><identifier>PMID: 20236187</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Bone Density Conservation Agents - administration & dosage ; Bone Density Conservation Agents - pharmacology ; Bone Matrix - transplantation ; Bone Regeneration - drug effects ; Calcium Phosphates - pharmacology ; Dentistry ; guided bone regeneration ; Injections ; Male ; parathyroid hormone ; Parathyroid Hormone - administration & dosage ; Parathyroid Hormone - pharmacology ; Random Allocation ; rat calvaria model ; Rats ; Rats, Sprague-Dawley ; Skull - surgery ; tissue engineering ; β-tricalcium phosphate</subject><ispartof>Journal of clinical periodontology, 2010-05, Vol.37 (5), p.419-426</ispartof><rights>2010 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4397-85f9a920b5c1ae50c2a3d96aa1ce416be26f806e4dc043ea3391d9aa93dbe1013</citedby><cites>FETCH-LOGICAL-c4397-85f9a920b5c1ae50c2a3d96aa1ce416be26f806e4dc043ea3391d9aa93dbe1013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-051X.2010.01547.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-051X.2010.01547.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20236187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yun, Jonathan I.</creatorcontrib><creatorcontrib>Wikesjö, Ulf ME</creatorcontrib><creatorcontrib>Borke, James L.</creatorcontrib><creatorcontrib>Bisch, Frederick C.</creatorcontrib><creatorcontrib>Lewis, Jill E.</creatorcontrib><creatorcontrib>Herold, Robert W.</creatorcontrib><creatorcontrib>Swiec, Gary D.</creatorcontrib><creatorcontrib>Wood, Joseph C.</creatorcontrib><creatorcontrib>McPherson III, James C.</creatorcontrib><title>Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model</title><title>Journal of clinical periodontology</title><addtitle>J Clin Periodontol</addtitle><description>Yun JI, Wikesjö UME, Borke JL, Bisch FC, Lewis JE, Herold RW, Swiec GD, Wood JC, McPherson III JC. Effect of systemic parathyroid hormone (1–34) and a β‐tricalcium phosphate biomaterial on local bone formation in a critical‐size rat calvarial defect model. J Clin Periodontol 2010; 37: 419–426 doi: 10.1111/j.1600‐051X.2010.01547.x
Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1–34) (PTH), a surgically implanted synthetic β‐tricalcium phosphate (β‐TCP) bone biomaterial serving as a matrix to support new bone formation.
Materials and Methods: Critical‐size, 8 mm, calvarial through‐and‐through osteotomy defects were surgically created in 100 adult male Sprague–Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β‐TCP, β‐TCP, or particulate human demineralized freeze‐dried bone (DFDB), and sham‐surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post‐surgery for radiographic and histometric analysis.
Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β‐TCP (15.7±2.4%), β‐TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham‐surgery control (10.0±1.5%) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0%) compared with PTH/β‐TCP (22.4±3.0%), β‐TCP (21.3±4.4%), and with the sham‐surgery control (23.8±4.2%) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3%) to 8 weeks (32.0±3.2%) (p<0.006). The PTH/β‐TCP and β‐TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β‐TCP biomaterial.
Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β‐TCP biomaterial.</description><subject>Animals</subject><subject>Bone Density Conservation Agents - administration & dosage</subject><subject>Bone Density Conservation Agents - pharmacology</subject><subject>Bone Matrix - transplantation</subject><subject>Bone Regeneration - drug effects</subject><subject>Calcium Phosphates - pharmacology</subject><subject>Dentistry</subject><subject>guided bone regeneration</subject><subject>Injections</subject><subject>Male</subject><subject>parathyroid hormone</subject><subject>Parathyroid Hormone - administration & dosage</subject><subject>Parathyroid Hormone - pharmacology</subject><subject>Random Allocation</subject><subject>rat calvaria model</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Skull - surgery</subject><subject>tissue engineering</subject><subject>β-tricalcium phosphate</subject><issn>0303-6979</issn><issn>1600-051X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-O0zAQxi0EYrsLr4B8Aw4p4zh_6gsSqrq7oBUgBAL1Yk2cieqS1MFOod134SV4EJ4Jp116BV_GM_6-31j6GOMCpiKeF-upKAASyMWXaQpxCiLPyunuHpucHu6zCUiQSaFKdcbOQ1gDiFJK-ZCdpZDKQszKCfu5aBoyA3cND_swUGcN79HjsNp7Z2u-cr5zG-LPRCKz5xw3NUf--1cyeGuwNXbb8X7lQr_CgXhlXRert9hyt-GtixJejfYmYnCwcWg3EWC8HUZ_Euwt8biNx-Y7How1HT7UuZraR-xBg22gx3f1gn26XHycXyc3765ez1_dJCaTqkxmeaNQpVDlRiDlYFKUtSoQhaFMFBWlRTODgrLaQCYJpVSiVohK1hUJEPKCPT1ye---bSkMurPBUNvihtw26DIr0jIFgH8rpRSgZDkyZ0el8S4ET43uve3Q77UAPaao13oMS49h6TFFfUhR76L1yd2SbdVRfTL-jS0KXh4FP2xL-_8G6zfz94vxGgHJEWBj5rsTAP1XXZSyzPXnt1c6XeaX12K51B_kH8LFvUI</recordid><startdate>201005</startdate><enddate>201005</enddate><creator>Yun, Jonathan I.</creator><creator>Wikesjö, Ulf ME</creator><creator>Borke, James L.</creator><creator>Bisch, Frederick C.</creator><creator>Lewis, Jill E.</creator><creator>Herold, Robert W.</creator><creator>Swiec, Gary D.</creator><creator>Wood, Joseph C.</creator><creator>McPherson III, James C.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>201005</creationdate><title>Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model</title><author>Yun, Jonathan I. ; Wikesjö, Ulf ME ; Borke, James L. ; Bisch, Frederick C. ; Lewis, Jill E. ; Herold, Robert W. ; Swiec, Gary D. ; Wood, Joseph C. ; McPherson III, James C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4397-85f9a920b5c1ae50c2a3d96aa1ce416be26f806e4dc043ea3391d9aa93dbe1013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Bone Density Conservation Agents - administration & dosage</topic><topic>Bone Density Conservation Agents - pharmacology</topic><topic>Bone Matrix - transplantation</topic><topic>Bone Regeneration - drug effects</topic><topic>Calcium Phosphates - pharmacology</topic><topic>Dentistry</topic><topic>guided bone regeneration</topic><topic>Injections</topic><topic>Male</topic><topic>parathyroid hormone</topic><topic>Parathyroid Hormone - administration & dosage</topic><topic>Parathyroid Hormone - pharmacology</topic><topic>Random Allocation</topic><topic>rat calvaria model</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Skull - surgery</topic><topic>tissue engineering</topic><topic>β-tricalcium phosphate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yun, Jonathan I.</creatorcontrib><creatorcontrib>Wikesjö, Ulf ME</creatorcontrib><creatorcontrib>Borke, James L.</creatorcontrib><creatorcontrib>Bisch, Frederick C.</creatorcontrib><creatorcontrib>Lewis, Jill E.</creatorcontrib><creatorcontrib>Herold, Robert W.</creatorcontrib><creatorcontrib>Swiec, Gary D.</creatorcontrib><creatorcontrib>Wood, Joseph C.</creatorcontrib><creatorcontrib>McPherson III, James C.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Journal of clinical periodontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yun, Jonathan I.</au><au>Wikesjö, Ulf ME</au><au>Borke, James L.</au><au>Bisch, Frederick C.</au><au>Lewis, Jill E.</au><au>Herold, Robert W.</au><au>Swiec, Gary D.</au><au>Wood, Joseph C.</au><au>McPherson III, James C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model</atitle><jtitle>Journal of clinical periodontology</jtitle><addtitle>J Clin Periodontol</addtitle><date>2010-05</date><risdate>2010</risdate><volume>37</volume><issue>5</issue><spage>419</spage><epage>426</epage><pages>419-426</pages><issn>0303-6979</issn><eissn>1600-051X</eissn><abstract>Yun JI, Wikesjö UME, Borke JL, Bisch FC, Lewis JE, Herold RW, Swiec GD, Wood JC, McPherson III JC. Effect of systemic parathyroid hormone (1–34) and a β‐tricalcium phosphate biomaterial on local bone formation in a critical‐size rat calvarial defect model. J Clin Periodontol 2010; 37: 419–426 doi: 10.1111/j.1600‐051X.2010.01547.x
Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1–34) (PTH), a surgically implanted synthetic β‐tricalcium phosphate (β‐TCP) bone biomaterial serving as a matrix to support new bone formation.
Materials and Methods: Critical‐size, 8 mm, calvarial through‐and‐through osteotomy defects were surgically created in 100 adult male Sprague–Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β‐TCP, β‐TCP, or particulate human demineralized freeze‐dried bone (DFDB), and sham‐surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post‐surgery for radiographic and histometric analysis.
Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β‐TCP (15.7±2.4%), β‐TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham‐surgery control (10.0±1.5%) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0%) compared with PTH/β‐TCP (22.4±3.0%), β‐TCP (21.3±4.4%), and with the sham‐surgery control (23.8±4.2%) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3%) to 8 weeks (32.0±3.2%) (p<0.006). The PTH/β‐TCP and β‐TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β‐TCP biomaterial.
Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β‐TCP biomaterial.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20236187</pmid><doi>10.1111/j.1600-051X.2010.01547.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Bone Density Conservation Agents - administration & dosage Bone Density Conservation Agents - pharmacology Bone Matrix - transplantation Bone Regeneration - drug effects Calcium Phosphates - pharmacology Dentistry guided bone regeneration Injections Male parathyroid hormone Parathyroid Hormone - administration & dosage Parathyroid Hormone - pharmacology Random Allocation rat calvaria model Rats Rats, Sprague-Dawley Skull - surgery tissue engineering β-tricalcium phosphate |
| title | Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model |
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