An Ultramicrochemical Test for Mycoplasmal Contamination of Cultured Cells

A sensitive ultramicrochemical enzyme test for mycoplasmal contamination of cultured cells, based on the determination of the activity of adenosine phosphorylase, is described. The test was performed by assaying the enzymatic conversion of$[8-^{14}C] adenine$and ribose-1-phosphate to$[8-^{14}C] aden...

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Veröffentlicht in:	In Vitro 1979-02, Vol.15 (2), p.103-108
Hauptverfasser:	M. P. Uitendaal, C. H. M. M. De Bruyn, Hatanaka, M., P. Hösli
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell culture techniques Cell Line Cell lines Cultured cells Enzymes Fibroblasts Fibroblasts - microbiology Gout - genetics HeLa cells Humans Hypoxanthine Phosphoribosyltransferase - analysis Hypoxia Lesch-Nyhan Syndrome Methods Mycoplasma Mycoplasma - enzymology Mycoplasma - isolation & purification Plasma cells Prenatal diagnosis Purine-Nucleoside Phosphorylase - analysis Purine-Nucleoside Phosphorylase - metabolism Skin
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