The diagnosis of proventricular dilatation disease: Use of a Western blot assay to detect antibodies against avian Borna virus
Avian Borna virus (ABV) has recently been shown to be the causal agent of proventricular dilatation disease (PDD) a lethal neurologic disease of captive psittacines and other birds. An immunoblot assay was used to detect the presence of antibodies against avian Borna virus in the serum of affected b...
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| Veröffentlicht in: | Veterinary microbiology 2010-07, Vol.143 (2), p.196-201 |
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| creator | Villanueva, Itamar Gray, Patricia Mirhosseini, Negin Payne, Susan Hoppes, Sharman Honkavuori, Kirsi S. Briese, Thomas Turner, Debra Tizard, Ian |
| description | Avian Borna virus (ABV) has recently been shown to be the causal agent of proventricular dilatation disease (PDD) a lethal neurologic disease of captive psittacines and other birds. An immunoblot assay was used to detect the presence of antibodies against avian Borna virus in the serum of affected birds. A lysate from ABV-infected duck embryo fibroblasts served as a source of antigen. The assay was used to test for the presence of antibodies to ABV in 117 birds. Thirty of these birds had biopsy or necropsy-confirmed proventricular dilatation disease (PDD), while the remaining 87 birds were apparently healthy or were suffering from diseases other than PDD. Sera from 27 of the 30 PDD cases (90%) contained antibodies to ABV. Seventy-three (84%) of the apparently “healthy” birds were seronegative. Additionally, sera from seven macaws and one parrot trapped in the Peruvian Amazon were seronegative. Positive sera recognized the bornaviral nucleoprotein (N-protein). While the presence of antibodies to ABV largely corresponded with the development of clinical PDD, 14 apparently healthy normal birds possessed detectable antibodies to ABV. The existence of a carrier state was confirmed when 13 of 15 apparently healthy cockatiels were shown by PCR to have detectable ABV RNA in their feces. Western blot assays may be of significant assistance in diagnosing proventricular dilatation disease. Many apparently healthy birds may however be seronegative while, at the same time, shedding ABV in their feces. |
| doi_str_mv | 10.1016/j.vetmic.2009.11.041 |
| format | Article |
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An immunoblot assay was used to detect the presence of antibodies against avian Borna virus in the serum of affected birds. A lysate from ABV-infected duck embryo fibroblasts served as a source of antigen. The assay was used to test for the presence of antibodies to ABV in 117 birds. Thirty of these birds had biopsy or necropsy-confirmed proventricular dilatation disease (PDD), while the remaining 87 birds were apparently healthy or were suffering from diseases other than PDD. Sera from 27 of the 30 PDD cases (90%) contained antibodies to ABV. Seventy-three (84%) of the apparently “healthy” birds were seronegative. Additionally, sera from seven macaws and one parrot trapped in the Peruvian Amazon were seronegative. Positive sera recognized the bornaviral nucleoprotein (N-protein). While the presence of antibodies to ABV largely corresponded with the development of clinical PDD, 14 apparently healthy normal birds possessed detectable antibodies to ABV. The existence of a carrier state was confirmed when 13 of 15 apparently healthy cockatiels were shown by PCR to have detectable ABV RNA in their feces. Western blot assays may be of significant assistance in diagnosing proventricular dilatation disease. Many apparently healthy birds may however be seronegative while, at the same time, shedding ABV in their feces.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2009.11.041</identifier><identifier>PMID: 20036080</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Viral - blood ; antibody detection ; Avian Borna virus ; aviary birds ; bioassays ; Biological and medical sciences ; bird diseases ; Bird Diseases - blood ; Bird Diseases - immunology ; Bird Diseases - virology ; blood serum ; Blotting, Western - veterinary ; Borna disease ; Borna Disease - blood ; Borna Disease - diagnosis ; Borna disease virus ; Borna disease virus - immunology ; carrier state ; central nervous system diseases ; disease detection ; disease diagnosis ; disease transmission ; etiology ; Fundamental and applied biological sciences. Psychology ; hematologic tests ; Microbiology ; parrots ; Proventricular dilatation disease ; Psittacidae ; Psittaciformes ; Psittacine ; Sensitivity and Specificity ; Serologic Tests - veterinary ; Serology ; validity ; Western blot ; Western blotting</subject><ispartof>Veterinary microbiology, 2010-07, Vol.143 (2), p.196-201</ispartof><rights>2009 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>(c) 2009 Elsevier B.V. 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An immunoblot assay was used to detect the presence of antibodies against avian Borna virus in the serum of affected birds. A lysate from ABV-infected duck embryo fibroblasts served as a source of antigen. The assay was used to test for the presence of antibodies to ABV in 117 birds. Thirty of these birds had biopsy or necropsy-confirmed proventricular dilatation disease (PDD), while the remaining 87 birds were apparently healthy or were suffering from diseases other than PDD. Sera from 27 of the 30 PDD cases (90%) contained antibodies to ABV. Seventy-three (84%) of the apparently “healthy” birds were seronegative. Additionally, sera from seven macaws and one parrot trapped in the Peruvian Amazon were seronegative. Positive sera recognized the bornaviral nucleoprotein (N-protein). While the presence of antibodies to ABV largely corresponded with the development of clinical PDD, 14 apparently healthy normal birds possessed detectable antibodies to ABV. The existence of a carrier state was confirmed when 13 of 15 apparently healthy cockatiels were shown by PCR to have detectable ABV RNA in their feces. Western blot assays may be of significant assistance in diagnosing proventricular dilatation disease. Many apparently healthy birds may however be seronegative while, at the same time, shedding ABV in their feces.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>antibody detection</subject><subject>Avian Borna virus</subject><subject>aviary birds</subject><subject>bioassays</subject><subject>Biological and medical sciences</subject><subject>bird diseases</subject><subject>Bird Diseases - blood</subject><subject>Bird Diseases - immunology</subject><subject>Bird Diseases - virology</subject><subject>blood serum</subject><subject>Blotting, Western - veterinary</subject><subject>Borna disease</subject><subject>Borna Disease - blood</subject><subject>Borna Disease - diagnosis</subject><subject>Borna disease virus</subject><subject>Borna disease virus - immunology</subject><subject>carrier state</subject><subject>central nervous system diseases</subject><subject>disease detection</subject><subject>disease diagnosis</subject><subject>disease transmission</subject><subject>etiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hematologic tests</subject><subject>Microbiology</subject><subject>parrots</subject><subject>Proventricular dilatation disease</subject><subject>Psittacidae</subject><subject>Psittaciformes</subject><subject>Psittacine</subject><subject>Sensitivity and Specificity</subject><subject>Serologic Tests - veterinary</subject><subject>Serology</subject><subject>validity</subject><subject>Western blot</subject><subject>Western blotting</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P1DAMhisEYoeFf4AgF8SpQ5K2acoBCVZ8SStxYEccIzdxh4w6yRK3lfbCbyejDnCDU2LneW3Hb1E8FXwruFCvDtsFp6O3W8l5txViy2txr9gI3ValbGp5v9jwqtWlEFVzUTwiOnDO607xh8VFllSKa74pft58R-Y87EMkTywO7DbFBcOUvJ1HSPlthAkmH0O-EgLha7YjPJHAviFNmALrxzgxIII7NkXmcEKb4zD5PjqPxGAPPlBOLR4CexdTALb4NNPj4sEAI-GT83lZ7D68v7n6VF5_-fj56u11aRtRTWWrO-uUgk5jq3krGmG1awYUvep7pR3kvFRO5U1IQOHqTnau003fVzkEVV0WL9e6-Xc_5jy1OXqyOI4QMM5k2lrJqmnq7v9kVXOpai0yWa-kTZEo4WBukz9CujOCm5NF5mBWi8zJIiOEyRZl2bNzg7k_ovsj-u1JBl6cASAL45AgWE9_OamV5KrN3POVGyAa2KfM7L5KLioudN026lTpzUpgXu3iMRmyHoNF51N2yLjo_z3rL40Bu4E</recordid><startdate>20100714</startdate><enddate>20100714</enddate><creator>Villanueva, Itamar</creator><creator>Gray, Patricia</creator><creator>Mirhosseini, Negin</creator><creator>Payne, Susan</creator><creator>Hoppes, Sharman</creator><creator>Honkavuori, Kirsi S.</creator><creator>Briese, Thomas</creator><creator>Turner, Debra</creator><creator>Tizard, Ian</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20100714</creationdate><title>The diagnosis of proventricular dilatation disease: Use of a Western blot assay to detect antibodies against avian Borna virus</title><author>Villanueva, Itamar ; Gray, Patricia ; Mirhosseini, Negin ; Payne, Susan ; Hoppes, Sharman ; Honkavuori, Kirsi S. ; Briese, Thomas ; Turner, Debra ; Tizard, Ian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-789cd66a98e7807151c8d5fe1b6bb68da8e726d60092ae1d4929d985bb3ae1a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>antibody detection</topic><topic>Avian Borna virus</topic><topic>aviary birds</topic><topic>bioassays</topic><topic>Biological and medical sciences</topic><topic>bird diseases</topic><topic>Bird Diseases - blood</topic><topic>Bird Diseases - immunology</topic><topic>Bird Diseases - virology</topic><topic>blood serum</topic><topic>Blotting, Western - veterinary</topic><topic>Borna disease</topic><topic>Borna Disease - blood</topic><topic>Borna Disease - diagnosis</topic><topic>Borna disease virus</topic><topic>Borna disease virus - immunology</topic><topic>carrier state</topic><topic>central nervous system diseases</topic><topic>disease detection</topic><topic>disease diagnosis</topic><topic>disease transmission</topic><topic>etiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hematologic tests</topic><topic>Microbiology</topic><topic>parrots</topic><topic>Proventricular dilatation disease</topic><topic>Psittacidae</topic><topic>Psittaciformes</topic><topic>Psittacine</topic><topic>Sensitivity and Specificity</topic><topic>Serologic Tests - veterinary</topic><topic>Serology</topic><topic>validity</topic><topic>Western blot</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Villanueva, Itamar</creatorcontrib><creatorcontrib>Gray, Patricia</creatorcontrib><creatorcontrib>Mirhosseini, Negin</creatorcontrib><creatorcontrib>Payne, Susan</creatorcontrib><creatorcontrib>Hoppes, Sharman</creatorcontrib><creatorcontrib>Honkavuori, Kirsi S.</creatorcontrib><creatorcontrib>Briese, Thomas</creatorcontrib><creatorcontrib>Turner, Debra</creatorcontrib><creatorcontrib>Tizard, Ian</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Villanueva, Itamar</au><au>Gray, Patricia</au><au>Mirhosseini, Negin</au><au>Payne, Susan</au><au>Hoppes, Sharman</au><au>Honkavuori, Kirsi S.</au><au>Briese, Thomas</au><au>Turner, Debra</au><au>Tizard, Ian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The diagnosis of proventricular dilatation disease: Use of a Western blot assay to detect antibodies against avian Borna virus</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2010-07-14</date><risdate>2010</risdate><volume>143</volume><issue>2</issue><spage>196</spage><epage>201</epage><pages>196-201</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Avian Borna virus (ABV) has recently been shown to be the causal agent of proventricular dilatation disease (PDD) a lethal neurologic disease of captive psittacines and other birds. An immunoblot assay was used to detect the presence of antibodies against avian Borna virus in the serum of affected birds. A lysate from ABV-infected duck embryo fibroblasts served as a source of antigen. The assay was used to test for the presence of antibodies to ABV in 117 birds. Thirty of these birds had biopsy or necropsy-confirmed proventricular dilatation disease (PDD), while the remaining 87 birds were apparently healthy or were suffering from diseases other than PDD. Sera from 27 of the 30 PDD cases (90%) contained antibodies to ABV. Seventy-three (84%) of the apparently “healthy” birds were seronegative. Additionally, sera from seven macaws and one parrot trapped in the Peruvian Amazon were seronegative. Positive sera recognized the bornaviral nucleoprotein (N-protein). While the presence of antibodies to ABV largely corresponded with the development of clinical PDD, 14 apparently healthy normal birds possessed detectable antibodies to ABV. The existence of a carrier state was confirmed when 13 of 15 apparently healthy cockatiels were shown by PCR to have detectable ABV RNA in their feces. Western blot assays may be of significant assistance in diagnosing proventricular dilatation disease. Many apparently healthy birds may however be seronegative while, at the same time, shedding ABV in their feces.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20036080</pmid><doi>10.1016/j.vetmic.2009.11.041</doi><tpages>6</tpages></addata></record> |
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| ispartof | Veterinary microbiology, 2010-07, Vol.143 (2), p.196-201 |
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| subjects | Animals Antibodies, Viral - blood antibody detection Avian Borna virus aviary birds bioassays Biological and medical sciences bird diseases Bird Diseases - blood Bird Diseases - immunology Bird Diseases - virology blood serum Blotting, Western - veterinary Borna disease Borna Disease - blood Borna Disease - diagnosis Borna disease virus Borna disease virus - immunology carrier state central nervous system diseases disease detection disease diagnosis disease transmission etiology Fundamental and applied biological sciences. Psychology hematologic tests Microbiology parrots Proventricular dilatation disease Psittacidae Psittaciformes Psittacine Sensitivity and Specificity Serologic Tests - veterinary Serology validity Western blot Western blotting |
| title | The diagnosis of proventricular dilatation disease: Use of a Western blot assay to detect antibodies against avian Borna virus |
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