Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells
In this work, an ultrasound-assisted procedure for the extraction of Lingzhi polysaccharides was established. Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters (ultrasonic frequency ( X1), extraction temperature ( X2), extraction time ( X3), and ra...
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| Veröffentlicht in: | Carbohydrate polymers 2010-05, Vol.80 (3), p.944-948 |
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| container_title | Carbohydrate polymers |
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| creator | Chen, XiaoPing Wang, WanXiang Li, ShuiBing Xue, JinLing Fan, LiJun Sheng, ZhongJi Chen, YouGuo |
| description | In this work, an ultrasound-assisted procedure for the extraction of Lingzhi polysaccharides was established. Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters (ultrasonic frequency (
X1), extraction temperature (
X2), extraction time (
X3), and ratio of water to raw material (
X4)) for enhancing the forward extraction efficiency of Lingzhi polysaccharides by implementing a three-level, four-variable Box–Behnken experimental design. The independent variable with the largest effect on response was
X1 (ultrasonic frequency), followed by
X2 (extraction temperature) and
X3 (extraction time). The optimum extraction conditions were found to be ultrasonic frequency 8
kHz, extraction temperature 95
°C, extraction time 3
h and ratio of water to raw material 12. Under these conditions, the forward extraction efficiency of Lingzhi polysaccharides can increase from 42% to 75%. Pharmacological experiment showed that Lingzhi polysaccharides could decrease CyclinB1 mRNA expression in CaSki cells and inhibit CaSki and HeLa cells proliferation. Results indicated that Lingzhi polysaccharides possessed strong antitumor activity. |
| doi_str_mv | 10.1016/j.carbpol.2010.01.010 |
| format | Article |
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X1), extraction temperature (
X2), extraction time (
X3), and ratio of water to raw material (
X4)) for enhancing the forward extraction efficiency of Lingzhi polysaccharides by implementing a three-level, four-variable Box–Behnken experimental design. The independent variable with the largest effect on response was
X1 (ultrasonic frequency), followed by
X2 (extraction temperature) and
X3 (extraction time). The optimum extraction conditions were found to be ultrasonic frequency 8
kHz, extraction temperature 95
°C, extraction time 3
h and ratio of water to raw material 12. Under these conditions, the forward extraction efficiency of Lingzhi polysaccharides can increase from 42% to 75%. Pharmacological experiment showed that Lingzhi polysaccharides could decrease CyclinB1 mRNA expression in CaSki cells and inhibit CaSki and HeLa cells proliferation. Results indicated that Lingzhi polysaccharides possessed strong antitumor activity.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2010.01.010</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Antineoplastic agents ; Applied sciences ; Biological and medical sciences ; CaSki ; Exact sciences and technology ; General aspects ; HeLa ; Lingzhi polysaccharides ; Medical sciences ; Natural polymers ; Optimization ; Pharmacology. Drug treatments ; Physicochemistry of polymers ; Response surface methodology (RSM) ; Starch and polysaccharides ; Ultrasonic-assisted extraction</subject><ispartof>Carbohydrate polymers, 2010-05, Vol.80 (3), p.944-948</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-f22748c7a4bacf345174117eb2ee5cafd51383403da9a137c33ac02eedb4bd893</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2010.01.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22579975$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, XiaoPing</creatorcontrib><creatorcontrib>Wang, WanXiang</creatorcontrib><creatorcontrib>Li, ShuiBing</creatorcontrib><creatorcontrib>Xue, JinLing</creatorcontrib><creatorcontrib>Fan, LiJun</creatorcontrib><creatorcontrib>Sheng, ZhongJi</creatorcontrib><creatorcontrib>Chen, YouGuo</creatorcontrib><title>Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells</title><title>Carbohydrate polymers</title><description>In this work, an ultrasound-assisted procedure for the extraction of Lingzhi polysaccharides was established. Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters (ultrasonic frequency (
X1), extraction temperature (
X2), extraction time (
X3), and ratio of water to raw material (
X4)) for enhancing the forward extraction efficiency of Lingzhi polysaccharides by implementing a three-level, four-variable Box–Behnken experimental design. The independent variable with the largest effect on response was
X1 (ultrasonic frequency), followed by
X2 (extraction temperature) and
X3 (extraction time). The optimum extraction conditions were found to be ultrasonic frequency 8
kHz, extraction temperature 95
°C, extraction time 3
h and ratio of water to raw material 12. Under these conditions, the forward extraction efficiency of Lingzhi polysaccharides can increase from 42% to 75%. Pharmacological experiment showed that Lingzhi polysaccharides could decrease CyclinB1 mRNA expression in CaSki cells and inhibit CaSki and HeLa cells proliferation. Results indicated that Lingzhi polysaccharides possessed strong antitumor activity.</description><subject>Antineoplastic agents</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>CaSki</subject><subject>Exact sciences and technology</subject><subject>General aspects</subject><subject>HeLa</subject><subject>Lingzhi polysaccharides</subject><subject>Medical sciences</subject><subject>Natural polymers</subject><subject>Optimization</subject><subject>Pharmacology. Drug treatments</subject><subject>Physicochemistry of polymers</subject><subject>Response surface methodology (RSM)</subject><subject>Starch and polysaccharides</subject><subject>Ultrasonic-assisted extraction</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFUcFu2zAMNYYVWNb2EwboMuzkTLLkyD4NQ7FuAwL0sp4FWqIaBY7liXKx9Gf2q1OQbNcJBESRj3wiX1W9E3wtuNh83K8tpGGO47rhJcZFMf6qWolO97WQSr2uVlwoVXcbod9Ub4n2vJyN4Kvq98OcwyG8QA5xYtGzZcwJKC6Tq4EoUEbH8FeJ2b-IbZieXnaBFcIjgbU7SMEhsYVKgiWkOU6EjJbkwSI7YN5FF8f4dGQwORYysTDtwhByTEeG3qPNrLS2mJ6DhZFZmIpf3uNIN9WVh5Hw9nJfV4_3X37cfau3D1-_333e1lZJnWvfNFp1VoMawHqpWqGVEBqHBrG14F0rZCcVlw56EFJbKcHyknSDGlzXy-vqw7nvnOLPBSmbQ6DTD2DCuJDRatM0faNkQbZnpE2RKKE3cwoHSEcjuDnpYfbmooc56WG4KMZL3fsLA1CZ0qcyZaB_xU3T6r7XbcF9OuOwjPscMBmyActGXEhlU8bF8B-mP5fgqSw</recordid><startdate>20100505</startdate><enddate>20100505</enddate><creator>Chen, XiaoPing</creator><creator>Wang, WanXiang</creator><creator>Li, ShuiBing</creator><creator>Xue, JinLing</creator><creator>Fan, LiJun</creator><creator>Sheng, ZhongJi</creator><creator>Chen, YouGuo</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100505</creationdate><title>Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells</title><author>Chen, XiaoPing ; Wang, WanXiang ; Li, ShuiBing ; Xue, JinLing ; Fan, LiJun ; Sheng, ZhongJi ; Chen, YouGuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-f22748c7a4bacf345174117eb2ee5cafd51383403da9a137c33ac02eedb4bd893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Antineoplastic agents</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>CaSki</topic><topic>Exact sciences and technology</topic><topic>General aspects</topic><topic>HeLa</topic><topic>Lingzhi polysaccharides</topic><topic>Medical sciences</topic><topic>Natural polymers</topic><topic>Optimization</topic><topic>Pharmacology. Drug treatments</topic><topic>Physicochemistry of polymers</topic><topic>Response surface methodology (RSM)</topic><topic>Starch and polysaccharides</topic><topic>Ultrasonic-assisted extraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, XiaoPing</creatorcontrib><creatorcontrib>Wang, WanXiang</creatorcontrib><creatorcontrib>Li, ShuiBing</creatorcontrib><creatorcontrib>Xue, JinLing</creatorcontrib><creatorcontrib>Fan, LiJun</creatorcontrib><creatorcontrib>Sheng, ZhongJi</creatorcontrib><creatorcontrib>Chen, YouGuo</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, XiaoPing</au><au>Wang, WanXiang</au><au>Li, ShuiBing</au><au>Xue, JinLing</au><au>Fan, LiJun</au><au>Sheng, ZhongJi</au><au>Chen, YouGuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells</atitle><jtitle>Carbohydrate polymers</jtitle><date>2010-05-05</date><risdate>2010</risdate><volume>80</volume><issue>3</issue><spage>944</spage><epage>948</epage><pages>944-948</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>In this work, an ultrasound-assisted procedure for the extraction of Lingzhi polysaccharides was established. Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters (ultrasonic frequency (
X1), extraction temperature (
X2), extraction time (
X3), and ratio of water to raw material (
X4)) for enhancing the forward extraction efficiency of Lingzhi polysaccharides by implementing a three-level, four-variable Box–Behnken experimental design. The independent variable with the largest effect on response was
X1 (ultrasonic frequency), followed by
X2 (extraction temperature) and
X3 (extraction time). The optimum extraction conditions were found to be ultrasonic frequency 8
kHz, extraction temperature 95
°C, extraction time 3
h and ratio of water to raw material 12. Under these conditions, the forward extraction efficiency of Lingzhi polysaccharides can increase from 42% to 75%. Pharmacological experiment showed that Lingzhi polysaccharides could decrease CyclinB1 mRNA expression in CaSki cells and inhibit CaSki and HeLa cells proliferation. Results indicated that Lingzhi polysaccharides possessed strong antitumor activity.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2010.01.010</doi><tpages>5</tpages></addata></record> |
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| ispartof | Carbohydrate polymers, 2010-05, Vol.80 (3), p.944-948 |
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| language | eng |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | Antineoplastic agents Applied sciences Biological and medical sciences CaSki Exact sciences and technology General aspects HeLa Lingzhi polysaccharides Medical sciences Natural polymers Optimization Pharmacology. Drug treatments Physicochemistry of polymers Response surface methodology (RSM) Starch and polysaccharides Ultrasonic-assisted extraction |
| title | Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells |
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