Nanotransportation system for cholera toxin in Vibrio cholerae 01
Vibrio cholerae ( V. cholerae ) cholera toxin (CT), which causes a severe watery diarrheal illness, is secreted via the type II secretion machinery; it remains unclear, however, how this toxin is transported toward the machinery. In this study, we determined that the pH-dependent intrabacterial tran...
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| Veröffentlicht in: | Medical molecular morphology 2009-03, Vol.42 (1), p.40-46 |
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| creator | Aoki, Hiroaki Wu, Hong Nakano, Takashi Ooi, Yukimasa Daikoku, Eriko Kohno, Takehiro Matsushita, Tomoyo Sano, Kouichi |
| description | Vibrio cholerae
(
V. cholerae
) cholera toxin (CT), which causes a severe watery diarrheal illness, is secreted via the type II secretion machinery; it remains unclear, however, how this toxin is transported toward the machinery. In this study, we determined that the pH-dependent intrabacterial transport system correlates with the priming of CT secretion by
V. cholerae
. The secretion and production of
V. cholerae
treated at different pHs were examined by enzyme immunoassay. The localization of the CT was analyzed by immunoelectron microscopy. The CT secretion level rapidly increases in the alkaline-pH-treated
V. cholerae
but does so more slowly in neutral- and acidic-pH-treated
V. cholerae
. The CT was found to be densely localized near the membrane in the alkaline-pH-treated bacterial cytoplasm, suggesting that the CT shifts from the center to the peripheral portion of the cytoplasm following an extracellular rise in pH. The shift was observed in
V. cholerae
treated at alkaline pH for more than 10 min. The pH treatment did not enhance CT production at the same stage at which secretion and intrabacterial transport of the CT were enhanced. We propose that
V. cholerae
possesses a pH-dependent intrabacterial nanotransportation system that probably accelerates priming for CT secretion. |
| doi_str_mv | 10.1007/s00795-008-0431-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_746228708</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>746228708</sourcerecordid><originalsourceid>FETCH-LOGICAL-c496t-cc2e65c2cc5aa49320710717760d5f723bd1db71d2fe18975d73c183554398413</originalsourceid><addsrcrecordid>eNp1kE9PwyAYh4nRuDn9AF5M48VTlRdogeOy-C8xelGvhFKqXdoyoU22by9Lp0tMDG-AwPP-IA9C54CvAWN-E-IksxRjkWJGIV0foCmIHKfApDz83Qs8QSchLDGmPCfZMZqAJJIxCVM0f9ad673uwsr5Xve165KwCb1tk8r5xHy6xnqd9G5dd0ms97rwtfs5twmGU3RU6SbYs906Q293t6-Lh_Tp5f5xMX9KDZN5nxpDbJ4ZYkymNZOUYA6xOM9xmVWc0KKEsuBQksqCkDwrOTUgaJYxKgUDOkNXY-7Ku6_Bhl61dTC2aXRn3RAUZzkhgmMRycs_5NINvoufUxSzPA4pIwQjZLwLwdtKrXzdar9RgNXWrhrtqmhXbe2qdey52AUPRWvLfcdOZwTICIR41X1Yv3_5_9RvMOSDjA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>304646499</pqid></control><display><type>article</type><title>Nanotransportation system for cholera toxin in Vibrio cholerae 01</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Aoki, Hiroaki ; Wu, Hong ; Nakano, Takashi ; Ooi, Yukimasa ; Daikoku, Eriko ; Kohno, Takehiro ; Matsushita, Tomoyo ; Sano, Kouichi</creator><creatorcontrib>Aoki, Hiroaki ; Wu, Hong ; Nakano, Takashi ; Ooi, Yukimasa ; Daikoku, Eriko ; Kohno, Takehiro ; Matsushita, Tomoyo ; Sano, Kouichi</creatorcontrib><description>Vibrio cholerae
(
V. cholerae
) cholera toxin (CT), which causes a severe watery diarrheal illness, is secreted via the type II secretion machinery; it remains unclear, however, how this toxin is transported toward the machinery. In this study, we determined that the pH-dependent intrabacterial transport system correlates with the priming of CT secretion by
V. cholerae
. The secretion and production of
V. cholerae
treated at different pHs were examined by enzyme immunoassay. The localization of the CT was analyzed by immunoelectron microscopy. The CT secretion level rapidly increases in the alkaline-pH-treated
V. cholerae
but does so more slowly in neutral- and acidic-pH-treated
V. cholerae
. The CT was found to be densely localized near the membrane in the alkaline-pH-treated bacterial cytoplasm, suggesting that the CT shifts from the center to the peripheral portion of the cytoplasm following an extracellular rise in pH. The shift was observed in
V. cholerae
treated at alkaline pH for more than 10 min. The pH treatment did not enhance CT production at the same stage at which secretion and intrabacterial transport of the CT were enhanced. We propose that
V. cholerae
possesses a pH-dependent intrabacterial nanotransportation system that probably accelerates priming for CT secretion.</description><identifier>ISSN: 1860-1480</identifier><identifier>EISSN: 1860-1499</identifier><identifier>DOI: 10.1007/s00795-008-0431-x</identifier><identifier>PMID: 19294491</identifier><identifier>CODEN: MELMEJ</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Anatomy ; Bacterial Proteins - metabolism ; Biological Transport, Active ; Cell Membrane - metabolism ; Cholera ; Cholera Toxin - metabolism ; Enzymes ; Hydrogen-Ion Concentration ; Immunohistochemistry ; Medicine ; Medicine & Public Health ; Metal Nanoparticles ; Microscopy ; Microscopy, Immunoelectron ; Models, Biological ; Molecular Medicine ; Morphology ; Original Paper ; Pathology ; Receptors, Cell Surface - metabolism ; Toxicology ; Vibrio cholerae ; Vibrio cholerae O1 - metabolism ; Vibrio cholerae O1 - ultrastructure</subject><ispartof>Medical molecular morphology, 2009-03, Vol.42 (1), p.40-46</ispartof><rights>The Japanese Society for Clinical Molecular Morphology 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-cc2e65c2cc5aa49320710717760d5f723bd1db71d2fe18975d73c183554398413</citedby><cites>FETCH-LOGICAL-c496t-cc2e65c2cc5aa49320710717760d5f723bd1db71d2fe18975d73c183554398413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00795-008-0431-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00795-008-0431-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19294491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aoki, Hiroaki</creatorcontrib><creatorcontrib>Wu, Hong</creatorcontrib><creatorcontrib>Nakano, Takashi</creatorcontrib><creatorcontrib>Ooi, Yukimasa</creatorcontrib><creatorcontrib>Daikoku, Eriko</creatorcontrib><creatorcontrib>Kohno, Takehiro</creatorcontrib><creatorcontrib>Matsushita, Tomoyo</creatorcontrib><creatorcontrib>Sano, Kouichi</creatorcontrib><title>Nanotransportation system for cholera toxin in Vibrio cholerae 01</title><title>Medical molecular morphology</title><addtitle>Med Mol Morphol</addtitle><addtitle>Med Mol Morphol</addtitle><description>Vibrio cholerae
(
V. cholerae
) cholera toxin (CT), which causes a severe watery diarrheal illness, is secreted via the type II secretion machinery; it remains unclear, however, how this toxin is transported toward the machinery. In this study, we determined that the pH-dependent intrabacterial transport system correlates with the priming of CT secretion by
V. cholerae
. The secretion and production of
V. cholerae
treated at different pHs were examined by enzyme immunoassay. The localization of the CT was analyzed by immunoelectron microscopy. The CT secretion level rapidly increases in the alkaline-pH-treated
V. cholerae
but does so more slowly in neutral- and acidic-pH-treated
V. cholerae
. The CT was found to be densely localized near the membrane in the alkaline-pH-treated bacterial cytoplasm, suggesting that the CT shifts from the center to the peripheral portion of the cytoplasm following an extracellular rise in pH. The shift was observed in
V. cholerae
treated at alkaline pH for more than 10 min. The pH treatment did not enhance CT production at the same stage at which secretion and intrabacterial transport of the CT were enhanced. We propose that
V. cholerae
possesses a pH-dependent intrabacterial nanotransportation system that probably accelerates priming for CT secretion.</description><subject>Anatomy</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biological Transport, Active</subject><subject>Cell Membrane - metabolism</subject><subject>Cholera</subject><subject>Cholera Toxin - metabolism</subject><subject>Enzymes</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunohistochemistry</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metal Nanoparticles</subject><subject>Microscopy</subject><subject>Microscopy, Immunoelectron</subject><subject>Models, Biological</subject><subject>Molecular Medicine</subject><subject>Morphology</subject><subject>Original Paper</subject><subject>Pathology</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Toxicology</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae O1 - metabolism</subject><subject>Vibrio cholerae O1 - ultrastructure</subject><issn>1860-1480</issn><issn>1860-1499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kE9PwyAYh4nRuDn9AF5M48VTlRdogeOy-C8xelGvhFKqXdoyoU22by9Lp0tMDG-AwPP-IA9C54CvAWN-E-IksxRjkWJGIV0foCmIHKfApDz83Qs8QSchLDGmPCfZMZqAJJIxCVM0f9ad673uwsr5Xve165KwCb1tk8r5xHy6xnqd9G5dd0ms97rwtfs5twmGU3RU6SbYs906Q293t6-Lh_Tp5f5xMX9KDZN5nxpDbJ4ZYkymNZOUYA6xOM9xmVWc0KKEsuBQksqCkDwrOTUgaJYxKgUDOkNXY-7Ku6_Bhl61dTC2aXRn3RAUZzkhgmMRycs_5NINvoufUxSzPA4pIwQjZLwLwdtKrXzdar9RgNXWrhrtqmhXbe2qdey52AUPRWvLfcdOZwTICIR41X1Yv3_5_9RvMOSDjA</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Aoki, Hiroaki</creator><creator>Wu, Hong</creator><creator>Nakano, Takashi</creator><creator>Ooi, Yukimasa</creator><creator>Daikoku, Eriko</creator><creator>Kohno, Takehiro</creator><creator>Matsushita, Tomoyo</creator><creator>Sano, Kouichi</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QL</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20090301</creationdate><title>Nanotransportation system for cholera toxin in Vibrio cholerae 01</title><author>Aoki, Hiroaki ; Wu, Hong ; Nakano, Takashi ; Ooi, Yukimasa ; Daikoku, Eriko ; Kohno, Takehiro ; Matsushita, Tomoyo ; Sano, Kouichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-cc2e65c2cc5aa49320710717760d5f723bd1db71d2fe18975d73c183554398413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anatomy</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biological Transport, Active</topic><topic>Cell Membrane - metabolism</topic><topic>Cholera</topic><topic>Cholera Toxin - metabolism</topic><topic>Enzymes</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunohistochemistry</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metal Nanoparticles</topic><topic>Microscopy</topic><topic>Microscopy, Immunoelectron</topic><topic>Models, Biological</topic><topic>Molecular Medicine</topic><topic>Morphology</topic><topic>Original Paper</topic><topic>Pathology</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Toxicology</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae O1 - metabolism</topic><topic>Vibrio cholerae O1 - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aoki, Hiroaki</creatorcontrib><creatorcontrib>Wu, Hong</creatorcontrib><creatorcontrib>Nakano, Takashi</creatorcontrib><creatorcontrib>Ooi, Yukimasa</creatorcontrib><creatorcontrib>Daikoku, Eriko</creatorcontrib><creatorcontrib>Kohno, Takehiro</creatorcontrib><creatorcontrib>Matsushita, Tomoyo</creatorcontrib><creatorcontrib>Sano, Kouichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Medical molecular morphology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aoki, Hiroaki</au><au>Wu, Hong</au><au>Nakano, Takashi</au><au>Ooi, Yukimasa</au><au>Daikoku, Eriko</au><au>Kohno, Takehiro</au><au>Matsushita, Tomoyo</au><au>Sano, Kouichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanotransportation system for cholera toxin in Vibrio cholerae 01</atitle><jtitle>Medical molecular morphology</jtitle><stitle>Med Mol Morphol</stitle><addtitle>Med Mol Morphol</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>42</volume><issue>1</issue><spage>40</spage><epage>46</epage><pages>40-46</pages><issn>1860-1480</issn><eissn>1860-1499</eissn><coden>MELMEJ</coden><abstract>Vibrio cholerae
(
V. cholerae
) cholera toxin (CT), which causes a severe watery diarrheal illness, is secreted via the type II secretion machinery; it remains unclear, however, how this toxin is transported toward the machinery. In this study, we determined that the pH-dependent intrabacterial transport system correlates with the priming of CT secretion by
V. cholerae
. The secretion and production of
V. cholerae
treated at different pHs were examined by enzyme immunoassay. The localization of the CT was analyzed by immunoelectron microscopy. The CT secretion level rapidly increases in the alkaline-pH-treated
V. cholerae
but does so more slowly in neutral- and acidic-pH-treated
V. cholerae
. The CT was found to be densely localized near the membrane in the alkaline-pH-treated bacterial cytoplasm, suggesting that the CT shifts from the center to the peripheral portion of the cytoplasm following an extracellular rise in pH. The shift was observed in
V. cholerae
treated at alkaline pH for more than 10 min. The pH treatment did not enhance CT production at the same stage at which secretion and intrabacterial transport of the CT were enhanced. We propose that
V. cholerae
possesses a pH-dependent intrabacterial nanotransportation system that probably accelerates priming for CT secretion.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>19294491</pmid><doi>10.1007/s00795-008-0431-x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Medical molecular morphology, 2009-03, Vol.42 (1), p.40-46 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Anatomy Bacterial Proteins - metabolism Biological Transport, Active Cell Membrane - metabolism Cholera Cholera Toxin - metabolism Enzymes Hydrogen-Ion Concentration Immunohistochemistry Medicine Medicine & Public Health Metal Nanoparticles Microscopy Microscopy, Immunoelectron Models, Biological Molecular Medicine Morphology Original Paper Pathology Receptors, Cell Surface - metabolism Toxicology Vibrio cholerae Vibrio cholerae O1 - metabolism Vibrio cholerae O1 - ultrastructure |
| title | Nanotransportation system for cholera toxin in Vibrio cholerae 01 |
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