Biochemical and morphological changes in dormant (“Nonculturable”) Mycobacterium smegmatis cells
Biochemical and morphological changes have been studied during transition of Mycobacterium smegmatis cells into their dormant (“nonculturable”) state. A significant fraction of the population of irreversibly “nonculturable” (NC) cells has a thicker cell wall, condensed cytoplasm, and a decreased num...
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| Veröffentlicht in: | Biochemistry (Moscow) 2010, Vol.75 (1), p.72-80 |
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| creator | Salina, E. G. Zhogina, Yu. A. Shleeva, M. O. Sorokoumova, G. M. Selishcheva, A. A. Kaprelyants, A. S. |
| description | Biochemical and morphological changes have been studied during transition of
Mycobacterium smegmatis
cells into their dormant (“nonculturable”) state. A significant fraction of the population of irreversibly “nonculturable” (NC) cells has a thicker cell wall, condensed cytoplasm, and a decreased number of ribosomes. The lipid contents in the NC cells are lower than in the metabolically active cells, with a relatively decreased amount of phospholipid and neutral lipid. Free mycolic acids, which are abundant in metabolically active cells, were not found in the NC cells. The NC forms are also characterized by decreased respiratory activity on endogenous substrates; however, the respiratory chain enzymes retain their activities in the isolated membranes. Activities of the Krebs cycle and glyoxylate cycle enzymes are markedly decreased. Despite a significant decrease in metabolic activity, NC cells possess membrane potential that seems to provide for reversibility of the NC state of mycobacteria, i.e. their capability of reactivating. |
| doi_str_mv | 10.1134/S0006297910010098 |
| format | Article |
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Mycobacterium smegmatis
cells into their dormant (“nonculturable”) state. A significant fraction of the population of irreversibly “nonculturable” (NC) cells has a thicker cell wall, condensed cytoplasm, and a decreased number of ribosomes. The lipid contents in the NC cells are lower than in the metabolically active cells, with a relatively decreased amount of phospholipid and neutral lipid. Free mycolic acids, which are abundant in metabolically active cells, were not found in the NC cells. The NC forms are also characterized by decreased respiratory activity on endogenous substrates; however, the respiratory chain enzymes retain their activities in the isolated membranes. Activities of the Krebs cycle and glyoxylate cycle enzymes are markedly decreased. Despite a significant decrease in metabolic activity, NC cells possess membrane potential that seems to provide for reversibility of the NC state of mycobacteria, i.e. their capability of reactivating.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/S0006297910010098</identifier><identifier>PMID: 20331426</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Bacteria ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Cells ; Cytoplasm ; Enzymes ; Isocitrate Dehydrogenase - metabolism ; Isocitrate Lyase - metabolism ; Life Sciences ; Lipids ; Malate Dehydrogenase - metabolism ; Microbiology ; Multienzyme Complexes - metabolism ; Mycobacterium smegmatis ; Mycobacterium smegmatis - chemistry ; Mycobacterium smegmatis - enzymology ; Mycobacterium smegmatis - ultrastructure ; NADH Dehydrogenase - metabolism ; NADH, NADPH Oxidoreductases - metabolism ; Phospholipids ; Phospholipids - chemistry ; Phospholipids - metabolism ; Tuberculosis</subject><ispartof>Biochemistry (Moscow), 2010, Vol.75 (1), p.72-80</ispartof><rights>Pleiades Publishing, Ltd. 2010</rights><rights>COPYRIGHT 2010 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-3377c883bf950e73ffbd3e019911659953e596450b6172e9d5f326ce8cc29a653</citedby><cites>FETCH-LOGICAL-c535t-3377c883bf950e73ffbd3e019911659953e596450b6172e9d5f326ce8cc29a653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0006297910010098$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0006297910010098$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20331426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salina, E. G.</creatorcontrib><creatorcontrib>Zhogina, Yu. A.</creatorcontrib><creatorcontrib>Shleeva, M. O.</creatorcontrib><creatorcontrib>Sorokoumova, G. M.</creatorcontrib><creatorcontrib>Selishcheva, A. A.</creatorcontrib><creatorcontrib>Kaprelyants, A. S.</creatorcontrib><title>Biochemical and morphological changes in dormant (“Nonculturable”) Mycobacterium smegmatis cells</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>Biochemical and morphological changes have been studied during transition of
Mycobacterium smegmatis
cells into their dormant (“nonculturable”) state. A significant fraction of the population of irreversibly “nonculturable” (NC) cells has a thicker cell wall, condensed cytoplasm, and a decreased number of ribosomes. The lipid contents in the NC cells are lower than in the metabolically active cells, with a relatively decreased amount of phospholipid and neutral lipid. Free mycolic acids, which are abundant in metabolically active cells, were not found in the NC cells. The NC forms are also characterized by decreased respiratory activity on endogenous substrates; however, the respiratory chain enzymes retain their activities in the isolated membranes. Activities of the Krebs cycle and glyoxylate cycle enzymes are markedly decreased. Despite a significant decrease in metabolic activity, NC cells possess membrane potential that seems to provide for reversibility of the NC state of mycobacteria, i.e. their capability of reactivating.</description><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Cells</subject><subject>Cytoplasm</subject><subject>Enzymes</subject><subject>Isocitrate Dehydrogenase - metabolism</subject><subject>Isocitrate Lyase - metabolism</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Malate Dehydrogenase - metabolism</subject><subject>Microbiology</subject><subject>Multienzyme Complexes - metabolism</subject><subject>Mycobacterium smegmatis</subject><subject>Mycobacterium smegmatis - chemistry</subject><subject>Mycobacterium smegmatis - enzymology</subject><subject>Mycobacterium smegmatis - ultrastructure</subject><subject>NADH Dehydrogenase - metabolism</subject><subject>NADH, NADPH Oxidoreductases - metabolism</subject><subject>Phospholipids</subject><subject>Phospholipids - chemistry</subject><subject>Phospholipids - metabolism</subject><subject>Tuberculosis</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFks1u1DAQxy0EokvhAbigCA7AIcXfiY-lKlCpwAE4W44z2XUV21s7OfTWB4GX65PUYQsVX8K2ZHnm9x_NeAahxwQfEML4q08YY0lVowjG5aj2DloRiduaYY7votXirhf_HnqQ81l5UqzYfbRHMWOEU7lC_WsX7Qa8s2asTOgrH9N2E8e4_m6xGxPWkCsXqj4mb8JUvbi6_PohBjuP05xMN8LV5beX1fsLGztjJ0hu9lX2sPZmcrmyMI75Ibo3mDHDo5t7H315c_z56F19-vHtydHhaW0FE1PNWNPYtmXdoASGhg1D1zPARClCpFBKMBBKcoE7SRoKqhcDo9JCay1VRgq2j57v4m5TPJ8hT9q7vGRgAsQ564ZLijlV9P8kW1YrZCGf_kaexTmFUoamjDJFW8UL9GwHrc0I2oUhTsnYJaQ-ZFw0DW9bXKiDv1Bl98v_xwCDK_ZfBGQnsCnmnGDQ2-S8SReaYL1MgP5jAormyU2-c-eh_6n40fIC0B2Qi6s0N90W9O-o1-12uWc</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Salina, E. 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G.</au><au>Zhogina, Yu. A.</au><au>Shleeva, M. O.</au><au>Sorokoumova, G. M.</au><au>Selishcheva, A. A.</au><au>Kaprelyants, A. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical and morphological changes in dormant (“Nonculturable”) Mycobacterium smegmatis cells</atitle><jtitle>Biochemistry (Moscow)</jtitle><stitle>Biochemistry Moscow</stitle><addtitle>Biochemistry (Mosc)</addtitle><date>2010</date><risdate>2010</risdate><volume>75</volume><issue>1</issue><spage>72</spage><epage>80</epage><pages>72-80</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>Biochemical and morphological changes have been studied during transition of
Mycobacterium smegmatis
cells into their dormant (“nonculturable”) state. A significant fraction of the population of irreversibly “nonculturable” (NC) cells has a thicker cell wall, condensed cytoplasm, and a decreased number of ribosomes. The lipid contents in the NC cells are lower than in the metabolically active cells, with a relatively decreased amount of phospholipid and neutral lipid. Free mycolic acids, which are abundant in metabolically active cells, were not found in the NC cells. The NC forms are also characterized by decreased respiratory activity on endogenous substrates; however, the respiratory chain enzymes retain their activities in the isolated membranes. Activities of the Krebs cycle and glyoxylate cycle enzymes are markedly decreased. Despite a significant decrease in metabolic activity, NC cells possess membrane potential that seems to provide for reversibility of the NC state of mycobacteria, i.e. their capability of reactivating.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><pmid>20331426</pmid><doi>10.1134/S0006297910010098</doi><tpages>9</tpages></addata></record> |
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| subjects | Bacteria Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Cells Cytoplasm Enzymes Isocitrate Dehydrogenase - metabolism Isocitrate Lyase - metabolism Life Sciences Lipids Malate Dehydrogenase - metabolism Microbiology Multienzyme Complexes - metabolism Mycobacterium smegmatis Mycobacterium smegmatis - chemistry Mycobacterium smegmatis - enzymology Mycobacterium smegmatis - ultrastructure NADH Dehydrogenase - metabolism NADH, NADPH Oxidoreductases - metabolism Phospholipids Phospholipids - chemistry Phospholipids - metabolism Tuberculosis |
| title | Biochemical and morphological changes in dormant (“Nonculturable”) Mycobacterium smegmatis cells |
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