Magnetic Cell Separation Using Antibody Binding with Protein A Expressed on Bacterial Magnetic Particles

Bacterial magnetic particles (BacMPs) are efficient platforms of proteins for surface display systems. In this study, mononuclear cells from peripheral blood were separated using BacMPs expressing protein A on the BacMP membrane surface (protein A−BacMPs), which were complexed with the Fc fragment o...

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Veröffentlicht in:	Analytical chemistry (Washington) 2004-11, Vol.76 (21), p.6207-6213
Hauptverfasser:	Kuhara, Motoki, Takeyama, Haruko, Tanaka, Tsuyoshi, Matsunaga, Tadashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Bacteria Binding sites Binding Sites, Antibody Biological and medical sciences Cells Chemistry Cytoplasm Enzyme-linked immunosorbent assay Exact sciences and technology Fundamental and applied biological sciences. Psychology Gene expression Immunoglobulin G Immunomagnetic Separation - methods Interleukin 6 Leukocytes (mononuclear) Lipopolysaccharides Magnetics Magnetism Magnetospirillum - chemistry Miscellaneous Monoclonal antibodies protein A Proteins Q1 Staining Staphylococcal Protein A - immunology Stem cells
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