Temperature Reduction in Cultures of hGM-CSF-expressing CHO Cells: Effect on Productivity and Product Quality

We have demonstrated that temperature reduction from 37 to 33 °C in the culture of a CHO cell line producing recombinant human granulocyte macrophage colony stimulating factor (CHO‐K1‐hGM‐CSF) leads to a reduced growth rate, increased cell viability, improved cellular productivity, and decreased cel...

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Veröffentlicht in:	Biotechnology progress 2005, Vol.21 (1), p.17-21
Hauptverfasser:	Bollati-Fogolín, Mariela, Forno, Guillermina, Nimtz, Manfred, Conradt, Harald S., Etcheverrigaray, Marina, Kratje, Ricardo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anion-exchange chromatography Cell culture Cell density Cell number Cell Survival - physiology Cells, Cultured CHO Cells Cricetinae Culture Techniques - methods Glycosylation Granulocyte-macrophage colony-stimulating factor Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis Granulocyte-Macrophage Colony-Stimulating Factor - isolation & purification Growth rate Humans L-Lactate Dehydrogenase - metabolism Metabolism Q1 Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Temperature Temperature effects Time Factors
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