Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells : analysis of 952 autologous peripheral blood stem cell transplantations
Cryopreservation and thawing of haematopoietic stem cells are associated with cell loss and infusion-related toxicities. We analysed viability, total nucleated cell (TNC) and CD34+ cell recovery, and infusion-related toxicities of 952 thawed and washed products. Mean TNC and CD34+ viable cells recov...
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| Veröffentlicht in: | Bone marrow transplantation (Basingstoke) 2007-11, Vol.40 (9), p.831-835 |
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| creator | FOIS, E DESMARTIN, M LOTZ, J.-P GALICIER, L RAFFOUX, E BENBUNAN, M MAROLLEAU, J.-P LARGHERO, J BENHAMIDA, S XAVIER, F VANNEAUX, V REA, D FERMAND, J.-P ARNULF, B MOUNIER, N ERTAULT, M |
| description | Cryopreservation and thawing of haematopoietic stem cells are associated with cell loss and infusion-related toxicities. We analysed viability, total nucleated cell (TNC) and CD34+ cell recovery, and infusion-related toxicities of 952 thawed and washed products. Mean TNC and CD34+ viable cells recoveries were 55.9+/-18.6 and 98.0+/-36.5%, respectively. Mean cell viability was 68.25+/-18.9%. TNC recovery was correlated with viability but independent of the initial nucleated cell concentration. No difference in TNC recovery or viability was observed according to underlying diseases, except for myeloma, for which these variables were significantly lower (P |
| doi_str_mv | 10.1038/sj.bmt.1705830 |
| format | Article |
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We analysed viability, total nucleated cell (TNC) and CD34+ cell recovery, and infusion-related toxicities of 952 thawed and washed products. Mean TNC and CD34+ viable cells recoveries were 55.9+/-18.6 and 98.0+/-36.5%, respectively. Mean cell viability was 68.25+/-18.9%. TNC recovery was correlated with viability but independent of the initial nucleated cell concentration. No difference in TNC recovery or viability was observed according to underlying diseases, except for myeloma, for which these variables were significantly lower (P<0.05). CD34+ cell recovery was not correlated with viability or CD34+ initial count and was similar for all diseases. Cryostorage duration was not associated with cell loss. Immediate adverse events occurred in 169 patients (19%) and were moderate (grade I or II) for the majority of patients. Clinical toxicity was associated with a higher infused cell number and the presence of clumps in infused bags. The washing procedure of cell products lead to a low rate of adverse events, but patients transplanted with high cell numbers or bags in which clumps were identified are predisposed to such complications.</description><identifier>ISSN: 0268-3369</identifier><identifier>EISSN: 1476-5365</identifier><identifier>DOI: 10.1038/sj.bmt.1705830</identifier><identifier>PMID: 17724443</identifier><identifier>CODEN: BMTRE9</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing Group</publisher><subject>Adverse events ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Antigens, CD34 ; Autografts ; Biological and medical sciences ; Bone marrow ; Bone marrow, stem cells transplantation. Graft versus host reaction ; CD34 antigen ; Cell Count ; Cell number ; Cell Survival ; Cell viability ; Clumps ; Complications ; Complications and side effects ; Cryopreservation ; Cryopreservation - methods ; Cryopreservation of organs, tissues, etc ; Diagnosis ; Graft versus host reaction ; Hematologic Diseases - complications ; Hematologic Diseases - therapy ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hemopoiesis ; Humans ; Medical sciences ; Methods ; Myeloma ; Neoplasms - complications ; Neoplasms - therapy ; Patients ; Peripheral blood ; Peripheral Blood Stem Cell Transplantation - adverse effects ; Peripheral Blood Stem Cell Transplantation - methods ; Progenitor cells ; Recovery ; Retrospective Studies ; Risk Factors ; Stem cell transplantation ; Stem cells ; Thawing ; Toxicity ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Transplantation ; Transplantation, Autologous</subject><ispartof>Bone marrow transplantation (Basingstoke), 2007-11, Vol.40 (9), p.831-835</ispartof><rights>2007 INIST-CNRS</rights><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Nov 2007</rights><rights>Nature Publishing Group 2007.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c548t-92b49c2df5eb27af613855cd206261bfc77f891d8656bdd76ed981e5a5a063983</citedby><cites>FETCH-LOGICAL-c548t-92b49c2df5eb27af613855cd206261bfc77f891d8656bdd76ed981e5a5a063983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19222367$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17724443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FOIS, E</creatorcontrib><creatorcontrib>DESMARTIN, M</creatorcontrib><creatorcontrib>LOTZ, J.-P</creatorcontrib><creatorcontrib>GALICIER, L</creatorcontrib><creatorcontrib>RAFFOUX, E</creatorcontrib><creatorcontrib>BENBUNAN, M</creatorcontrib><creatorcontrib>MAROLLEAU, J.-P</creatorcontrib><creatorcontrib>LARGHERO, J</creatorcontrib><creatorcontrib>BENHAMIDA, S</creatorcontrib><creatorcontrib>XAVIER, F</creatorcontrib><creatorcontrib>VANNEAUX, V</creatorcontrib><creatorcontrib>REA, D</creatorcontrib><creatorcontrib>FERMAND, J.-P</creatorcontrib><creatorcontrib>ARNULF, B</creatorcontrib><creatorcontrib>MOUNIER, N</creatorcontrib><creatorcontrib>ERTAULT, M</creatorcontrib><title>Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells : analysis of 952 autologous peripheral blood stem cell transplantations</title><title>Bone marrow transplantation (Basingstoke)</title><addtitle>Bone Marrow Transplant</addtitle><description>Cryopreservation and thawing of haematopoietic stem cells are associated with cell loss and infusion-related toxicities. We analysed viability, total nucleated cell (TNC) and CD34+ cell recovery, and infusion-related toxicities of 952 thawed and washed products. Mean TNC and CD34+ viable cells recoveries were 55.9+/-18.6 and 98.0+/-36.5%, respectively. Mean cell viability was 68.25+/-18.9%. TNC recovery was correlated with viability but independent of the initial nucleated cell concentration. No difference in TNC recovery or viability was observed according to underlying diseases, except for myeloma, for which these variables were significantly lower (P<0.05). CD34+ cell recovery was not correlated with viability or CD34+ initial count and was similar for all diseases. Cryostorage duration was not associated with cell loss. Immediate adverse events occurred in 169 patients (19%) and were moderate (grade I or II) for the majority of patients. Clinical toxicity was associated with a higher infused cell number and the presence of clumps in infused bags. The washing procedure of cell products lead to a low rate of adverse events, but patients transplanted with high cell numbers or bags in which clumps were identified are predisposed to such complications.</description><subject>Adverse events</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Antigens, CD34</subject><subject>Autografts</subject><subject>Biological and medical sciences</subject><subject>Bone marrow</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>CD34 antigen</subject><subject>Cell Count</subject><subject>Cell number</subject><subject>Cell Survival</subject><subject>Cell viability</subject><subject>Clumps</subject><subject>Complications</subject><subject>Complications and side effects</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation of organs, tissues, etc</subject><subject>Diagnosis</subject><subject>Graft versus host reaction</subject><subject>Hematologic Diseases - complications</subject><subject>Hematologic Diseases - therapy</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hemopoiesis</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Myeloma</subject><subject>Neoplasms - complications</subject><subject>Neoplasms - therapy</subject><subject>Patients</subject><subject>Peripheral blood</subject><subject>Peripheral Blood Stem Cell Transplantation - adverse effects</subject><subject>Peripheral Blood Stem Cell Transplantation - methods</subject><subject>Progenitor cells</subject><subject>Recovery</subject><subject>Retrospective Studies</subject><subject>Risk Factors</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Thawing</subject><subject>Toxicity</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>Transplantation</subject><subject>Transplantation, Autologous</subject><issn>0268-3369</issn><issn>1476-5365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kluL1DAYhoso7rh666UEF_XGGXNO6t2yeIIFQfQ6pGk6zZA2NUl3nV_k3zQzFkZlJRcJX573OyRvVT1FcIMgkW_SbtMMeYMEZJLAe9UKUcHXjHB2v1pBzOWaEF6fVY9S2kGIKIXsYXWGhMCUUrKqfn6xJtzYuH8NbpxunHd5D_TYAuPd6Iz2IIcfzhyioQO517e2Pd7f6tSXY6_toHOYgrPZGTDFsLWjyyECY71P4G2Btd8nlw76mmGg5xx82IY5gclGN_U2liqND6EFKdvhKAQ56jFNXo9ZZxfG9Lh60Gmf7JNlP6--vX_39erj-vrzh09Xl9drw6jM6xo3tDa47ZhtsNAdR0QyZloMOeao6YwQnaxRKznjTdsKbttaIss005CTWpLz6tXvvGWS77NNWQ0uHTrSoy0tK0E5YgJKUsiLf8hdmGMZNinMKUacQSgK9fy_FOKcylrgU6qt9la5sQtlfHMorC6RpJJRjFmhNndQZbV2cCaMtnMl_pfg5R-C3mqf-xT8fHzQOzObGFKKtlNTdIOOe4WgOthMpZ0qNlOLzYrg2TLV3Ay2PeGLrwrwYgF0Kibqym8al05cjTEmXJBf6mbbdw</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>FOIS, E</creator><creator>DESMARTIN, M</creator><creator>LOTZ, J.-P</creator><creator>GALICIER, L</creator><creator>RAFFOUX, E</creator><creator>BENBUNAN, M</creator><creator>MAROLLEAU, J.-P</creator><creator>LARGHERO, J</creator><creator>BENHAMIDA, S</creator><creator>XAVIER, F</creator><creator>VANNEAUX, V</creator><creator>REA, D</creator><creator>FERMAND, J.-P</creator><creator>ARNULF, B</creator><creator>MOUNIER, N</creator><creator>ERTAULT, M</creator><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20071101</creationdate><title>Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells : analysis of 952 autologous peripheral blood stem cell transplantations</title><author>FOIS, E ; DESMARTIN, M ; LOTZ, J.-P ; GALICIER, L ; RAFFOUX, E ; BENBUNAN, M ; MAROLLEAU, J.-P ; LARGHERO, J ; BENHAMIDA, S ; XAVIER, F ; VANNEAUX, V ; REA, D ; FERMAND, J.-P ; ARNULF, B ; MOUNIER, N ; ERTAULT, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-92b49c2df5eb27af613855cd206261bfc77f891d8656bdd76ed981e5a5a063983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adverse events</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Antigens, CD34</topic><topic>Autografts</topic><topic>Biological and medical sciences</topic><topic>Bone marrow</topic><topic>Bone marrow, stem cells transplantation. Graft versus host reaction</topic><topic>CD34 antigen</topic><topic>Cell Count</topic><topic>Cell number</topic><topic>Cell Survival</topic><topic>Cell viability</topic><topic>Clumps</topic><topic>Complications</topic><topic>Complications and side effects</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation of organs, tissues, etc</topic><topic>Diagnosis</topic><topic>Graft versus host reaction</topic><topic>Hematologic Diseases - complications</topic><topic>Hematologic Diseases - therapy</topic><topic>Hematopoietic stem cells</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hemopoiesis</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Methods</topic><topic>Myeloma</topic><topic>Neoplasms - complications</topic><topic>Neoplasms - therapy</topic><topic>Patients</topic><topic>Peripheral blood</topic><topic>Peripheral Blood Stem Cell Transplantation - adverse effects</topic><topic>Peripheral Blood Stem Cell Transplantation - methods</topic><topic>Progenitor cells</topic><topic>Recovery</topic><topic>Retrospective Studies</topic><topic>Risk Factors</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Thawing</topic><topic>Toxicity</topic><topic>Transfusions. Complications. Transfusion reactions. 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We analysed viability, total nucleated cell (TNC) and CD34+ cell recovery, and infusion-related toxicities of 952 thawed and washed products. Mean TNC and CD34+ viable cells recoveries were 55.9+/-18.6 and 98.0+/-36.5%, respectively. Mean cell viability was 68.25+/-18.9%. TNC recovery was correlated with viability but independent of the initial nucleated cell concentration. No difference in TNC recovery or viability was observed according to underlying diseases, except for myeloma, for which these variables were significantly lower (P<0.05). CD34+ cell recovery was not correlated with viability or CD34+ initial count and was similar for all diseases. Cryostorage duration was not associated with cell loss. Immediate adverse events occurred in 169 patients (19%) and were moderate (grade I or II) for the majority of patients. Clinical toxicity was associated with a higher infused cell number and the presence of clumps in infused bags. The washing procedure of cell products lead to a low rate of adverse events, but patients transplanted with high cell numbers or bags in which clumps were identified are predisposed to such complications.</abstract><cop>Basingstoke</cop><pub>Nature Publishing Group</pub><pmid>17724443</pmid><doi>10.1038/sj.bmt.1705830</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bone marrow transplantation (Basingstoke), 2007-11, Vol.40 (9), p.831-835 |
| issn | 0268-3369 1476-5365 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature; EZB-FREE-00999 freely available EZB journals |
| subjects | Adverse events Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Antigens, CD34 Autografts Biological and medical sciences Bone marrow Bone marrow, stem cells transplantation. Graft versus host reaction CD34 antigen Cell Count Cell number Cell Survival Cell viability Clumps Complications Complications and side effects Cryopreservation Cryopreservation - methods Cryopreservation of organs, tissues, etc Diagnosis Graft versus host reaction Hematologic Diseases - complications Hematologic Diseases - therapy Hematopoietic stem cells Hematopoietic Stem Cells - cytology Hemopoiesis Humans Medical sciences Methods Myeloma Neoplasms - complications Neoplasms - therapy Patients Peripheral blood Peripheral Blood Stem Cell Transplantation - adverse effects Peripheral Blood Stem Cell Transplantation - methods Progenitor cells Recovery Retrospective Studies Risk Factors Stem cell transplantation Stem cells Thawing Toxicity Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transplantation Transplantation, Autologous |
| title | Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells : analysis of 952 autologous peripheral blood stem cell transplantations |
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