Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha
This study aimed to investigate the characteristics of a spontaneous mutation that results in lower cellulose production by a Gluconacetobacter xylinus strain isolated from Kombucha. The growth of this mutant was initially observed in Hestrin Schramm (HS) static and agitated cultures but not in modi...
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| Veröffentlicht in: | Carbohydrate polymers 2010-04, Vol.80 (2), p.337-343 |
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| container_title | Carbohydrate polymers |
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| creator | Nguyen, Vu Tuan Flanagan, Bernadine Mikkelsen, Deirdre Ramirez, Santiago Rivas, Lucia Gidley, Michael J. Dykes, Gary A. |
| description | This study aimed to investigate the characteristics of a spontaneous mutation that results in lower cellulose production by a
Gluconacetobacter xylinus strain isolated from Kombucha. The growth of this mutant was initially observed in Hestrin Schramm (HS) static and agitated cultures but not in modified (higher nutrient) HS cultures, indicating different nutrient conditions may play a role in the selection of the spontaneous mutant. The mutant produced a significantly (
P
<
.05) lower amount of cellulose than the wild-type in HS static culture. Nuclear magnetic resonance and scanning electron microscopy indicated that the cellulose produced by the mutant had the same crystalline structure as that produced by the wild-type but had a lower density of fibrils. Two-dimensional gel electrophoresis of total proteins demonstrated the presence of the enzyme deoxythymidine diphosphate (dTDP)-4-dehydrorhamnose 3,5-epimerase in the mutant but not in the wild-type. This enzyme could be involved in the synthesis of acetan, which may reduce cellulose synthesis and be the basis for the characteristics of the mutant. |
| doi_str_mv | 10.1016/j.carbpol.2009.11.019 |
| format | Article |
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Gluconacetobacter xylinus strain isolated from Kombucha. The growth of this mutant was initially observed in Hestrin Schramm (HS) static and agitated cultures but not in modified (higher nutrient) HS cultures, indicating different nutrient conditions may play a role in the selection of the spontaneous mutant. The mutant produced a significantly (
P
<
.05) lower amount of cellulose than the wild-type in HS static culture. Nuclear magnetic resonance and scanning electron microscopy indicated that the cellulose produced by the mutant had the same crystalline structure as that produced by the wild-type but had a lower density of fibrils. Two-dimensional gel electrophoresis of total proteins demonstrated the presence of the enzyme deoxythymidine diphosphate (dTDP)-4-dehydrorhamnose 3,5-epimerase in the mutant but not in the wild-type. This enzyme could be involved in the synthesis of acetan, which may reduce cellulose synthesis and be the basis for the characteristics of the mutant.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2009.11.019</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Applied sciences ; Bacterial cellulose ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Cellulose and derivatives ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Gluconacetobacter xylinus ; Methods. Procedures. Technologies ; Mutation ; Natural polymers ; Physicochemistry of polymers</subject><ispartof>Carbohydrate polymers, 2010-04, Vol.80 (2), p.337-343</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-639b59b449ff9a8dc636c2eb43c24be10ddc6d9ad4f6072a16936d1c5eacad123</citedby><cites>FETCH-LOGICAL-c371t-639b59b449ff9a8dc636c2eb43c24be10ddc6d9ad4f6072a16936d1c5eacad123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0144861709006602$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22577090$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, Vu Tuan</creatorcontrib><creatorcontrib>Flanagan, Bernadine</creatorcontrib><creatorcontrib>Mikkelsen, Deirdre</creatorcontrib><creatorcontrib>Ramirez, Santiago</creatorcontrib><creatorcontrib>Rivas, Lucia</creatorcontrib><creatorcontrib>Gidley, Michael J.</creatorcontrib><creatorcontrib>Dykes, Gary A.</creatorcontrib><title>Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha</title><title>Carbohydrate polymers</title><description>This study aimed to investigate the characteristics of a spontaneous mutation that results in lower cellulose production by a
Gluconacetobacter xylinus strain isolated from Kombucha. The growth of this mutant was initially observed in Hestrin Schramm (HS) static and agitated cultures but not in modified (higher nutrient) HS cultures, indicating different nutrient conditions may play a role in the selection of the spontaneous mutant. The mutant produced a significantly (
P
<
.05) lower amount of cellulose than the wild-type in HS static culture. Nuclear magnetic resonance and scanning electron microscopy indicated that the cellulose produced by the mutant had the same crystalline structure as that produced by the wild-type but had a lower density of fibrils. Two-dimensional gel electrophoresis of total proteins demonstrated the presence of the enzyme deoxythymidine diphosphate (dTDP)-4-dehydrorhamnose 3,5-epimerase in the mutant but not in the wild-type. This enzyme could be involved in the synthesis of acetan, which may reduce cellulose synthesis and be the basis for the characteristics of the mutant.</description><subject>Applied sciences</subject><subject>Bacterial cellulose</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cellulose and derivatives</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gluconacetobacter xylinus</subject><subject>Methods. Procedures. Technologies</subject><subject>Mutation</subject><subject>Natural polymers</subject><subject>Physicochemistry of polymers</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkEGP1DAMhSMEEsPCT0DKBXFqids0nZwQWrELYiUOwDlynVRklDZDkgLz78kwI674Ysn6_Oz3GHsJogUB6s2hJUzTMYa2E0K3AK0A_YjtYD_qBnopH7OdACmbvYLxKXuW80HUUiB2bP1yjGvB1cUt82UrWHxceXJ5CyVzv_IQf7nEyYWwhZgdP6ZoN_pLTSeO_D5sFFckV-KEVCr7-xT8WtVySVgF5hQX_iku00bf8Tl7MmPI7sW137Bvd--_3n5oHj7ff7x999BQP0JpVK-nQU9S6nnWuLekekWdm2RPnZwcCFtHVqOVsxJjh6B0ryzQ4JDQQtffsNcX3fruj83lYhafzyYuTs0oFQxKa6jkcCEpxZyTm80x-QXTyYAw53jNwVzjNed4DYCp8da9V9cLmAnDnHAln_8td90wjkKLyr29cK7a_eldMpm8W8lZnxwVY6P_z6U_dROWxw</recordid><startdate>20100412</startdate><enddate>20100412</enddate><creator>Nguyen, Vu Tuan</creator><creator>Flanagan, Bernadine</creator><creator>Mikkelsen, Deirdre</creator><creator>Ramirez, Santiago</creator><creator>Rivas, Lucia</creator><creator>Gidley, Michael J.</creator><creator>Dykes, Gary A.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20100412</creationdate><title>Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha</title><author>Nguyen, Vu Tuan ; Flanagan, Bernadine ; Mikkelsen, Deirdre ; Ramirez, Santiago ; Rivas, Lucia ; Gidley, Michael J. ; Dykes, Gary A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-639b59b449ff9a8dc636c2eb43c24be10ddc6d9ad4f6072a16936d1c5eacad123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Applied sciences</topic><topic>Bacterial cellulose</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cellulose and derivatives</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gluconacetobacter xylinus</topic><topic>Methods. Procedures. Technologies</topic><topic>Mutation</topic><topic>Natural polymers</topic><topic>Physicochemistry of polymers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, Vu Tuan</creatorcontrib><creatorcontrib>Flanagan, Bernadine</creatorcontrib><creatorcontrib>Mikkelsen, Deirdre</creatorcontrib><creatorcontrib>Ramirez, Santiago</creatorcontrib><creatorcontrib>Rivas, Lucia</creatorcontrib><creatorcontrib>Gidley, Michael J.</creatorcontrib><creatorcontrib>Dykes, Gary A.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, Vu Tuan</au><au>Flanagan, Bernadine</au><au>Mikkelsen, Deirdre</au><au>Ramirez, Santiago</au><au>Rivas, Lucia</au><au>Gidley, Michael J.</au><au>Dykes, Gary A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha</atitle><jtitle>Carbohydrate polymers</jtitle><date>2010-04-12</date><risdate>2010</risdate><volume>80</volume><issue>2</issue><spage>337</spage><epage>343</epage><pages>337-343</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>This study aimed to investigate the characteristics of a spontaneous mutation that results in lower cellulose production by a
Gluconacetobacter xylinus strain isolated from Kombucha. The growth of this mutant was initially observed in Hestrin Schramm (HS) static and agitated cultures but not in modified (higher nutrient) HS cultures, indicating different nutrient conditions may play a role in the selection of the spontaneous mutant. The mutant produced a significantly (
P
<
.05) lower amount of cellulose than the wild-type in HS static culture. Nuclear magnetic resonance and scanning electron microscopy indicated that the cellulose produced by the mutant had the same crystalline structure as that produced by the wild-type but had a lower density of fibrils. Two-dimensional gel electrophoresis of total proteins demonstrated the presence of the enzyme deoxythymidine diphosphate (dTDP)-4-dehydrorhamnose 3,5-epimerase in the mutant but not in the wild-type. This enzyme could be involved in the synthesis of acetan, which may reduce cellulose synthesis and be the basis for the characteristics of the mutant.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2009.11.019</doi><tpages>7</tpages></addata></record> |
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| ispartof | Carbohydrate polymers, 2010-04, Vol.80 (2), p.337-343 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Applied sciences Bacterial cellulose Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Cellulose and derivatives Exact sciences and technology Fundamental and applied biological sciences. Psychology Gluconacetobacter xylinus Methods. Procedures. Technologies Mutation Natural polymers Physicochemistry of polymers |
| title | Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha |
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