Puffer fish-based commercial fraud identification in a segment of cytochrome b region by PCR–RFLP analysis

To identify the mislabeled or fraudulently substituted toxic puffer fish in thermally processed fish products, a polymerase chain reaction (PCR) method using restriction sites and sequence analysis has been developed in this study. A 376-bp fragment of the cytochrome b gene was produced after PCR am...

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Veröffentlicht in:	Food chemistry 2010-08, Vol.121 (4), p.1305-1311
Hauptverfasser:	Hsieh, Cheng-Hong, Chang, Wen-Teish, Chang, Hebron C., Hsieh, Hung-Sheng, Chung, Yun-Lung, Hwang, Deng-Fwu
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences cytochrome b Cytochrome b gene Dry-dressed fish fillet equipment performance Fish and seafood industries fish processing food analysis Food authenticity food composition food contamination Food industries fraud Fundamental and applied biological sciences. Psychology heat treatment molecular sequence data nucleotide sequences PCR–RFLP polymerase chain reaction product authenticity Puffer fish-based product restriction fragment length polymorphism sequence analysis Species identification Tetraodontidae Thermal treatment product
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