Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts
Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the role of ACh in bone metabolism remains unclear. Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acety...
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| Veröffentlicht in: | FEBS letters 2010-02, Vol.584 (4), p.817-824 |
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| creator | Sato, Tsuyoshi Abe, Takahiro Chida, Dai Nakamoto, Norimichi Hori, Naoko Kokabu, Shoichiro Sakata, Yasuaki Tomaru, Yasuhisa Iwata, Takanori Usui, Michihiko Aiko, Katsuya Yoda, Tetsuya |
| description | Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the role of ACh in bone metabolism remains unclear. Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acetylcholine receptors (mAChRs) in osteoblasts. We detected mRNA expression of several nAChRs and mAChRs. Furthermore, we showed that cholinergic components were up-regulated and subunits/subtypes of acetylcholine receptors altered during osteoblast differentiation. To our knowledge, this is the first report demonstrating that osteoblasts express specific acetylcholine receptors and cholinergic components and that ACh plays a possible role in regulating the proliferation and differentiation of osteoblasts. |
| doi_str_mv | 10.1016/j.febslet.2010.01.001 |
| format | Article |
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Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acetylcholine receptors (mAChRs) in osteoblasts. We detected mRNA expression of several nAChRs and mAChRs. Furthermore, we showed that cholinergic components were up-regulated and subunits/subtypes of acetylcholine receptors altered during osteoblast differentiation. To our knowledge, this is the first report demonstrating that osteoblasts express specific acetylcholine receptors and cholinergic components and that ACh plays a possible role in regulating the proliferation and differentiation of osteoblasts.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2010.01.001</identifier><identifier>PMID: 20067796</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>3T3 Cells ; 5-bromo-2′-deoxyuridine ; Acetylcholine ; Acetylcholine - analogs & derivatives ; Acetylcholine - pharmacology ; acetylcholinesterase ; Acetylcholinesterase - genetics ; ACh ; AChE ; alkaline phosphatase ; Alkaline Phosphatase - metabolism ; ALP ; Animals ; Atr ; atropine ; Blotting, Western ; BrdU ; carbachol ; CCh ; Cell Proliferation - drug effects ; Cells, Cultured ; ChAT ; choline acetyltransferase ; Choline O-Acetyltransferase - genetics ; Cholinergic Agents - pharmacology ; Cholinergic component ; Cholinergic receptor ; CHT1 ; Cyclin D1 - genetics ; Cyclin D1 - metabolism ; Differentiation ; Dose-Response Relationship, Drug ; Gene Expression Regulation - drug effects ; high affinity choline transporter ; mAChRs ; Mec ; mecamylamine ; Mice ; Mus ; muscarine ; muscarinic acetylcholine receptors ; nAChRs ; Nic ; nicotine ; nicotinic acetylcholine receptors ; Osteoblast ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; pOB ; primary osteoblasts ; Proliferation ; Receptors, Cholinergic - genetics ; Receptors, Cholinergic - metabolism ; Receptors, Muscarinic - genetics ; Receptors, Muscarinic - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Symporters - genetics ; VAChT ; vasoactive intestinal peptide ; Vesicular Acetylcholine Transport Proteins - genetics ; vesicular acetylcholine transporter ; VIP</subject><ispartof>FEBS letters, 2010-02, Vol.584 (4), p.817-824</ispartof><rights>2010</rights><rights>FEBS Letters 584 (2010) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Crown Copyright 2010. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5740-fdffa8300690e13da879e1e42a3ada644ea9c1df12ef5c3262594fee7fcf4cc13</citedby><cites>FETCH-LOGICAL-c5740-fdffa8300690e13da879e1e42a3ada644ea9c1df12ef5c3262594fee7fcf4cc13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2010.01.001$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2010.01.001$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,778,782,1414,1430,3539,27911,27912,45561,45562,45982,46396,46820</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20067796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sato, Tsuyoshi</creatorcontrib><creatorcontrib>Abe, Takahiro</creatorcontrib><creatorcontrib>Chida, Dai</creatorcontrib><creatorcontrib>Nakamoto, Norimichi</creatorcontrib><creatorcontrib>Hori, Naoko</creatorcontrib><creatorcontrib>Kokabu, Shoichiro</creatorcontrib><creatorcontrib>Sakata, Yasuaki</creatorcontrib><creatorcontrib>Tomaru, Yasuhisa</creatorcontrib><creatorcontrib>Iwata, Takanori</creatorcontrib><creatorcontrib>Usui, Michihiko</creatorcontrib><creatorcontrib>Aiko, Katsuya</creatorcontrib><creatorcontrib>Yoda, Tetsuya</creatorcontrib><title>Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the role of ACh in bone metabolism remains unclear. Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acetylcholine receptors (mAChRs) in osteoblasts. We detected mRNA expression of several nAChRs and mAChRs. Furthermore, we showed that cholinergic components were up-regulated and subunits/subtypes of acetylcholine receptors altered during osteoblast differentiation. To our knowledge, this is the first report demonstrating that osteoblasts express specific acetylcholine receptors and cholinergic components and that ACh plays a possible role in regulating the proliferation and differentiation of osteoblasts.</description><subject>3T3 Cells</subject><subject>5-bromo-2′-deoxyuridine</subject><subject>Acetylcholine</subject><subject>Acetylcholine - analogs & derivatives</subject><subject>Acetylcholine - pharmacology</subject><subject>acetylcholinesterase</subject><subject>Acetylcholinesterase - genetics</subject><subject>ACh</subject><subject>AChE</subject><subject>alkaline phosphatase</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>ALP</subject><subject>Animals</subject><subject>Atr</subject><subject>atropine</subject><subject>Blotting, Western</subject><subject>BrdU</subject><subject>carbachol</subject><subject>CCh</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>ChAT</subject><subject>choline acetyltransferase</subject><subject>Choline O-Acetyltransferase - genetics</subject><subject>Cholinergic Agents - pharmacology</subject><subject>Cholinergic component</subject><subject>Cholinergic receptor</subject><subject>CHT1</subject><subject>Cyclin D1 - genetics</subject><subject>Cyclin D1 - metabolism</subject><subject>Differentiation</subject><subject>Dose-Response Relationship, Drug</subject><subject>Gene Expression Regulation - drug effects</subject><subject>high affinity choline transporter</subject><subject>mAChRs</subject><subject>Mec</subject><subject>mecamylamine</subject><subject>Mice</subject><subject>Mus</subject><subject>muscarine</subject><subject>muscarinic acetylcholine receptors</subject><subject>nAChRs</subject><subject>Nic</subject><subject>nicotine</subject><subject>nicotinic acetylcholine receptors</subject><subject>Osteoblast</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>pOB</subject><subject>primary osteoblasts</subject><subject>Proliferation</subject><subject>Receptors, Cholinergic - genetics</subject><subject>Receptors, Cholinergic - metabolism</subject><subject>Receptors, Muscarinic - genetics</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Symporters - genetics</subject><subject>VAChT</subject><subject>vasoactive intestinal peptide</subject><subject>Vesicular Acetylcholine Transport Proteins - genetics</subject><subject>vesicular acetylcholine transporter</subject><subject>VIP</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv2yAUx9G0aU27fYRWvu3k9GGwMaeprZq1UqUdtp0RwY-ViBgPSLd8--Em27Xlgnj8-PPgR8g5hSUF2l1ulhbXyWNeNlBqQJcA9A1Z0F6wmvGuf0sWpcLrVkh2Qk5T2kBZ91S-JycNQCeE7BZks9qNJrswal_F4LEKttIG896bx-DdiJUehyo_YoV_pogpFXRmjrvxpzNVRINTDjE9syZspzDimFPlCpoyhrXXKacP5J3VPuHH43xGfqxuv9_c1Q9fv9zfXD3UphUcajtYq3tWOpSAlA26FxIp8kYzPeiOc9TS0MHSBm1rWNM1reQWUVhjuTGUnZFPh9wphl87TFltXTLovR4x7JISvKNcAIeXScYkpxLmzPZAmhhSimjVFN1Wx72ioGYfaqOOPtTsQwFV8Hzu4njDbr3F4f-pfwIKcHcAfjuP-9elqtXtdfNtlju7pTCPdn7N50MUls99chhVMg5Hg4MrhrIagnuh27-D_LZu</recordid><startdate>20100219</startdate><enddate>20100219</enddate><creator>Sato, Tsuyoshi</creator><creator>Abe, Takahiro</creator><creator>Chida, Dai</creator><creator>Nakamoto, Norimichi</creator><creator>Hori, Naoko</creator><creator>Kokabu, Shoichiro</creator><creator>Sakata, Yasuaki</creator><creator>Tomaru, Yasuhisa</creator><creator>Iwata, Takanori</creator><creator>Usui, Michihiko</creator><creator>Aiko, Katsuya</creator><creator>Yoda, Tetsuya</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20100219</creationdate><title>Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts</title><author>Sato, Tsuyoshi ; Abe, Takahiro ; Chida, Dai ; Nakamoto, Norimichi ; Hori, Naoko ; Kokabu, Shoichiro ; Sakata, Yasuaki ; Tomaru, Yasuhisa ; Iwata, Takanori ; Usui, Michihiko ; Aiko, Katsuya ; Yoda, Tetsuya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5740-fdffa8300690e13da879e1e42a3ada644ea9c1df12ef5c3262594fee7fcf4cc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>3T3 Cells</topic><topic>5-bromo-2′-deoxyuridine</topic><topic>Acetylcholine</topic><topic>Acetylcholine - analogs & derivatives</topic><topic>Acetylcholine - pharmacology</topic><topic>acetylcholinesterase</topic><topic>Acetylcholinesterase - genetics</topic><topic>ACh</topic><topic>AChE</topic><topic>alkaline phosphatase</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>ALP</topic><topic>Animals</topic><topic>Atr</topic><topic>atropine</topic><topic>Blotting, Western</topic><topic>BrdU</topic><topic>carbachol</topic><topic>CCh</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>ChAT</topic><topic>choline acetyltransferase</topic><topic>Choline O-Acetyltransferase - genetics</topic><topic>Cholinergic Agents - pharmacology</topic><topic>Cholinergic component</topic><topic>Cholinergic receptor</topic><topic>CHT1</topic><topic>Cyclin D1 - genetics</topic><topic>Cyclin D1 - metabolism</topic><topic>Differentiation</topic><topic>Dose-Response Relationship, Drug</topic><topic>Gene Expression Regulation - drug effects</topic><topic>high affinity choline transporter</topic><topic>mAChRs</topic><topic>Mec</topic><topic>mecamylamine</topic><topic>Mice</topic><topic>Mus</topic><topic>muscarine</topic><topic>muscarinic acetylcholine receptors</topic><topic>nAChRs</topic><topic>Nic</topic><topic>nicotine</topic><topic>nicotinic acetylcholine receptors</topic><topic>Osteoblast</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - metabolism</topic><topic>pOB</topic><topic>primary osteoblasts</topic><topic>Proliferation</topic><topic>Receptors, Cholinergic - genetics</topic><topic>Receptors, Cholinergic - metabolism</topic><topic>Receptors, Muscarinic - genetics</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Symporters - genetics</topic><topic>VAChT</topic><topic>vasoactive intestinal peptide</topic><topic>Vesicular Acetylcholine Transport Proteins - genetics</topic><topic>vesicular acetylcholine transporter</topic><topic>VIP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sato, Tsuyoshi</creatorcontrib><creatorcontrib>Abe, Takahiro</creatorcontrib><creatorcontrib>Chida, Dai</creatorcontrib><creatorcontrib>Nakamoto, Norimichi</creatorcontrib><creatorcontrib>Hori, Naoko</creatorcontrib><creatorcontrib>Kokabu, Shoichiro</creatorcontrib><creatorcontrib>Sakata, Yasuaki</creatorcontrib><creatorcontrib>Tomaru, Yasuhisa</creatorcontrib><creatorcontrib>Iwata, Takanori</creatorcontrib><creatorcontrib>Usui, Michihiko</creatorcontrib><creatorcontrib>Aiko, Katsuya</creatorcontrib><creatorcontrib>Yoda, Tetsuya</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sato, Tsuyoshi</au><au>Abe, Takahiro</au><au>Chida, Dai</au><au>Nakamoto, Norimichi</au><au>Hori, Naoko</au><au>Kokabu, Shoichiro</au><au>Sakata, Yasuaki</au><au>Tomaru, Yasuhisa</au><au>Iwata, Takanori</au><au>Usui, Michihiko</au><au>Aiko, Katsuya</au><au>Yoda, Tetsuya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2010-02-19</date><risdate>2010</risdate><volume>584</volume><issue>4</issue><spage>817</spage><epage>824</epage><pages>817-824</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the role of ACh in bone metabolism remains unclear. Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acetylcholine receptors (mAChRs) in osteoblasts. We detected mRNA expression of several nAChRs and mAChRs. Furthermore, we showed that cholinergic components were up-regulated and subunits/subtypes of acetylcholine receptors altered during osteoblast differentiation. To our knowledge, this is the first report demonstrating that osteoblasts express specific acetylcholine receptors and cholinergic components and that ACh plays a possible role in regulating the proliferation and differentiation of osteoblasts.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>20067796</pmid><doi>10.1016/j.febslet.2010.01.001</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2010-02, Vol.584 (4), p.817-824 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Free Content; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 3T3 Cells 5-bromo-2′-deoxyuridine Acetylcholine Acetylcholine - analogs & derivatives Acetylcholine - pharmacology acetylcholinesterase Acetylcholinesterase - genetics ACh AChE alkaline phosphatase Alkaline Phosphatase - metabolism ALP Animals Atr atropine Blotting, Western BrdU carbachol CCh Cell Proliferation - drug effects Cells, Cultured ChAT choline acetyltransferase Choline O-Acetyltransferase - genetics Cholinergic Agents - pharmacology Cholinergic component Cholinergic receptor CHT1 Cyclin D1 - genetics Cyclin D1 - metabolism Differentiation Dose-Response Relationship, Drug Gene Expression Regulation - drug effects high affinity choline transporter mAChRs Mec mecamylamine Mice Mus muscarine muscarinic acetylcholine receptors nAChRs Nic nicotine nicotinic acetylcholine receptors Osteoblast Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - metabolism pOB primary osteoblasts Proliferation Receptors, Cholinergic - genetics Receptors, Cholinergic - metabolism Receptors, Muscarinic - genetics Receptors, Muscarinic - metabolism Reverse Transcriptase Polymerase Chain Reaction Symporters - genetics VAChT vasoactive intestinal peptide Vesicular Acetylcholine Transport Proteins - genetics vesicular acetylcholine transporter VIP |
| title | Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts |
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