Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion
Mitotic and meiotic chromosomes of several populations of Eurysternus caribaeus (Coleoptera: Scarabaeidae) were analysed through conventional staining, C-banding, base-specific fluorochromes, silver nitrate staining and fluorescent in situ hybridization (FISH). All specimens showed 2 n = 8 in their...
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| Veröffentlicht in: | Journal of genetics 2009-08, Vol.88 (2), p.177-182 |
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| creator | De Arcanjo, Amanda Paulino Cabral-De-Mello, Diogo Cavalcanti Silva, Ana Emília Barros E. De Moura, Rita De Cássia |
| description | Mitotic and meiotic chromosomes of several populations of
Eurysternus caribaeus
(Coleoptera: Scarabaeidae) were analysed through conventional staining, C-banding, base-specific fluorochromes, silver nitrate staining and fluorescent
in situ
hybridization (FISH). All specimens showed 2
n
= 8 in their karyotypes, with a neo-XY sex system (Y is a submetacentric and X a metacentric) and three pairs of submetacentric autosomes. The analysis of constitutive heterochromatin (CH) revealed small blocks located in the centromeric region of all chromosomes which do not present positive staining under the fluorochromes CMA3 and DAPI. Silver nitrate staining revealed that the nucleolar organizer region (NORs) is associated with the sex chromosomes. The FISH technique revealed that rDNA sites in the X and Y are different in size. Data from different populations indicate that the diploid number reduction (2
n
= 8) observed in
E. caribaeus
is established and presumably has preceded the dispersion of this species. Moreover, this reduction occasioned the translocation of rDNA sites to the sex chromosomes, X and Y, an uncommon pattern in Scarabaeidae that was observed for the first time by the FISH in this work. |
| doi_str_mv | 10.1007/s12041-009-0025-y |
| format | Article |
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Eurysternus caribaeus
(Coleoptera: Scarabaeidae) were analysed through conventional staining, C-banding, base-specific fluorochromes, silver nitrate staining and fluorescent
in situ
hybridization (FISH). All specimens showed 2
n
= 8 in their karyotypes, with a neo-XY sex system (Y is a submetacentric and X a metacentric) and three pairs of submetacentric autosomes. The analysis of constitutive heterochromatin (CH) revealed small blocks located in the centromeric region of all chromosomes which do not present positive staining under the fluorochromes CMA3 and DAPI. Silver nitrate staining revealed that the nucleolar organizer region (NORs) is associated with the sex chromosomes. The FISH technique revealed that rDNA sites in the X and Y are different in size. Data from different populations indicate that the diploid number reduction (2
n
= 8) observed in
E. caribaeus
is established and presumably has preceded the dispersion of this species. Moreover, this reduction occasioned the translocation of rDNA sites to the sex chromosomes, X and Y, an uncommon pattern in Scarabaeidae that was observed for the first time by the FISH in this work.</description><identifier>ISSN: 0022-1333</identifier><identifier>EISSN: 0973-7731</identifier><identifier>DOI: 10.1007/s12041-009-0025-y</identifier><identifier>PMID: 19700855</identifier><language>eng</language><publisher>India: Springer-Verlag</publisher><subject>Animal Genetics and Genomics ; Animals ; Biomedical and Life Sciences ; Cell division ; Chromosome Banding ; Coleoptera ; Coleoptera - genetics ; Cytogenetics ; Deoxyribonucleic acid ; Diploidy ; DNA ; Evolutionary Biology ; Fluorescence in situ hybridization ; Genes, Insect ; In Situ Hybridization, Fluorescence ; Insects ; Life Sciences ; Male ; Microbial Genetics and Genomics ; Nucleolus Organizer Region ; Plant Genetics and Genomics ; Research Article ; Scarabaeidae ; Species Specificity</subject><ispartof>Journal of genetics, 2009-08, Vol.88 (2), p.177-182</ispartof><rights>Indian Academy of Sciences 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-500967aed8d8423c5e68c4ad9ad876e1772f99bbd6523111de6b4de5fdb5ff613</citedby><cites>FETCH-LOGICAL-c444t-500967aed8d8423c5e68c4ad9ad876e1772f99bbd6523111de6b4de5fdb5ff613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12041-009-0025-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12041-009-0025-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19700855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Arcanjo, Amanda Paulino</creatorcontrib><creatorcontrib>Cabral-De-Mello, Diogo Cavalcanti</creatorcontrib><creatorcontrib>Silva, Ana Emília Barros E.</creatorcontrib><creatorcontrib>De Moura, Rita De Cássia</creatorcontrib><title>Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion</title><title>Journal of genetics</title><addtitle>J Genet</addtitle><addtitle>J Genet</addtitle><description>Mitotic and meiotic chromosomes of several populations of
Eurysternus caribaeus
(Coleoptera: Scarabaeidae) were analysed through conventional staining, C-banding, base-specific fluorochromes, silver nitrate staining and fluorescent
in situ
hybridization (FISH). All specimens showed 2
n
= 8 in their karyotypes, with a neo-XY sex system (Y is a submetacentric and X a metacentric) and three pairs of submetacentric autosomes. The analysis of constitutive heterochromatin (CH) revealed small blocks located in the centromeric region of all chromosomes which do not present positive staining under the fluorochromes CMA3 and DAPI. Silver nitrate staining revealed that the nucleolar organizer region (NORs) is associated with the sex chromosomes. The FISH technique revealed that rDNA sites in the X and Y are different in size. Data from different populations indicate that the diploid number reduction (2
n
= 8) observed in
E. caribaeus
is established and presumably has preceded the dispersion of this species. Moreover, this reduction occasioned the translocation of rDNA sites to the sex chromosomes, X and Y, an uncommon pattern in Scarabaeidae that was observed for the first time by the FISH in this work.</description><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Cell division</subject><subject>Chromosome Banding</subject><subject>Coleoptera</subject><subject>Coleoptera - genetics</subject><subject>Cytogenetics</subject><subject>Deoxyribonucleic acid</subject><subject>Diploidy</subject><subject>DNA</subject><subject>Evolutionary Biology</subject><subject>Fluorescence in situ hybridization</subject><subject>Genes, Insect</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Insects</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Microbial Genetics and Genomics</subject><subject>Nucleolus Organizer Region</subject><subject>Plant Genetics and Genomics</subject><subject>Research Article</subject><subject>Scarabaeidae</subject><subject>Species Specificity</subject><issn>0022-1333</issn><issn>0973-7731</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kcuO1DAQRS0EYoaGD2CDLBY8FgE_4jiZHWoND2kkFsDacuzK4FESN3aMCJ_EV1KhWwIhwcJyuXxulcuXkIecveCM6ZeZC1bzirEOl1DVeoucs07LSmvJb2PMhKi4lPKM3Mv5ZjtqJu6SM95pxlqlzsmP_brEa5hhCY66zzZZt0AK3-0S4kzjQC9LWjOm5pKpsyn0FjB6to8jxAPm7QX9gHmL-eAtPL-g8DV4mB1s6gzfKluWmOMEdCh5K2pnT304jDF4Opeph0QT-OJ-dTykEBNdIs0HcAEykhilTXif3BnsmOHBad-RT68vP-7fVlfv37zbv7qqXF3XS6XwOxptwbe-rYV0CprW1dZ31re6Aa61GLqu732jhOSce2j62oMafK-GoeFyR54e6x5S_FIgL2YK2cE42hliyUbXCHEpOiSf_JdstOraDh-xI4__Am9iSTNOYdAKIZXgNUL8CLkUc04wGPyMyabVcGY2v83Rb4MDms1vs6Lm0alw6SfwvxUngxEQRyDj1XwN6Y_O_6z6E0Zaukg</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>De Arcanjo, Amanda Paulino</creator><creator>Cabral-De-Mello, Diogo Cavalcanti</creator><creator>Silva, Ana Emília Barros E.</creator><creator>De Moura, Rita De Cássia</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090801</creationdate><title>Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion</title><author>De Arcanjo, Amanda Paulino ; Cabral-De-Mello, Diogo Cavalcanti ; Silva, Ana Emília Barros E. ; De Moura, Rita De Cássia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-500967aed8d8423c5e68c4ad9ad876e1772f99bbd6523111de6b4de5fdb5ff613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Cell division</topic><topic>Chromosome Banding</topic><topic>Coleoptera</topic><topic>Coleoptera - genetics</topic><topic>Cytogenetics</topic><topic>Deoxyribonucleic acid</topic><topic>Diploidy</topic><topic>DNA</topic><topic>Evolutionary Biology</topic><topic>Fluorescence in situ hybridization</topic><topic>Genes, Insect</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Insects</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Microbial Genetics and Genomics</topic><topic>Nucleolus Organizer Region</topic><topic>Plant Genetics and Genomics</topic><topic>Research Article</topic><topic>Scarabaeidae</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Arcanjo, Amanda Paulino</creatorcontrib><creatorcontrib>Cabral-De-Mello, Diogo Cavalcanti</creatorcontrib><creatorcontrib>Silva, Ana Emília Barros E.</creatorcontrib><creatorcontrib>De Moura, Rita De Cássia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Arcanjo, Amanda Paulino</au><au>Cabral-De-Mello, Diogo Cavalcanti</au><au>Silva, Ana Emília Barros E.</au><au>De Moura, Rita De Cássia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion</atitle><jtitle>Journal of genetics</jtitle><stitle>J Genet</stitle><addtitle>J Genet</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>88</volume><issue>2</issue><spage>177</spage><epage>182</epage><pages>177-182</pages><issn>0022-1333</issn><eissn>0973-7731</eissn><abstract>Mitotic and meiotic chromosomes of several populations of
Eurysternus caribaeus
(Coleoptera: Scarabaeidae) were analysed through conventional staining, C-banding, base-specific fluorochromes, silver nitrate staining and fluorescent
in situ
hybridization (FISH). All specimens showed 2
n
= 8 in their karyotypes, with a neo-XY sex system (Y is a submetacentric and X a metacentric) and three pairs of submetacentric autosomes. The analysis of constitutive heterochromatin (CH) revealed small blocks located in the centromeric region of all chromosomes which do not present positive staining under the fluorochromes CMA3 and DAPI. Silver nitrate staining revealed that the nucleolar organizer region (NORs) is associated with the sex chromosomes. The FISH technique revealed that rDNA sites in the X and Y are different in size. Data from different populations indicate that the diploid number reduction (2
n
= 8) observed in
E. caribaeus
is established and presumably has preceded the dispersion of this species. Moreover, this reduction occasioned the translocation of rDNA sites to the sex chromosomes, X and Y, an uncommon pattern in Scarabaeidae that was observed for the first time by the FISH in this work.</abstract><cop>India</cop><pub>Springer-Verlag</pub><pmid>19700855</pmid><doi>10.1007/s12041-009-0025-y</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of genetics, 2009-08, Vol.88 (2), p.177-182 |
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| language | eng |
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| source | MEDLINE; Indian Academy of Sciences; Springer Nature - Complete Springer Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animal Genetics and Genomics Animals Biomedical and Life Sciences Cell division Chromosome Banding Coleoptera Coleoptera - genetics Cytogenetics Deoxyribonucleic acid Diploidy DNA Evolutionary Biology Fluorescence in situ hybridization Genes, Insect In Situ Hybridization, Fluorescence Insects Life Sciences Male Microbial Genetics and Genomics Nucleolus Organizer Region Plant Genetics and Genomics Research Article Scarabaeidae Species Specificity |
| title | Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion |
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