A Thermostable Sugar-Binding Protein from the Archaeon Pyrococcus horikoshii as a Probe for the Development of a Stable Fluorescence Biosensor for Diabetic Patients

In this work is presented the first attempt to develop an innovative ultrastable protein‐based biosensor for blood glucose detections. The gene of a putative thermostable sugar‐binding protein has been cloned and expressed in E. coli. The recombinant protein has been purified to homogeneity by therm...

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Veröffentlicht in:	Biotechnology progress 2004, Vol.20 (5), p.1572-1577
Hauptverfasser:	Staiano, Maria, Sapio, MariaRosaria, Scognamiglio, Viviana, Marabotti, Anna, Facchiano, Angelo M., Bazzicalupo, Paolo, Rossi, Mose', d'Auria, Sabato
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity chromatography Archaea - metabolism Binding Sites Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Blood Glucose - analysis C.D Diabetes mellitus Diabetes Mellitus - blood Diabetes Mellitus - diagnosis Fluorescence Fundamental and applied biological sciences. Psychology Glucose - analysis Glucose - chemistry Humans Lectins - analysis Lectins - chemistry Lectins - genetics Macromolecular Substances - analysis Macromolecular Substances - chemistry Models, Chemical Models, Molecular Molecular Probes - chemistry Precipitation Protein Binding Protein Conformation Pyrococcus horikoshii Pyrococcus horikoshii - metabolism Q1 Q2 Recombinant Proteins - analysis Recombinant Proteins - chemistry Spectrometry, Fluorescence - methods
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