Improving the expression of mini-proinsulin in Pichia pastoris
Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 °C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedur...
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| Veröffentlicht in: | Biotechnology letters 2004-08, Vol.26 (16), p.1269-1272 |
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| container_title | Biotechnology letters |
| container_volume | 26 |
| creator | Pais-Chanfrau, J.M Garcia, Y Licor, L Besada, V Castellanos-Serra, L Cabello, C.I Hernandez, L Mansur, M Plana, L Hidalgo, A |
| description | Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 °C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l-1 in the culture supernatant after 160 h of growth. |
| doi_str_mv | 10.1023/B:BILE.0000044871.80632.f9 |
| format | Article |
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Using this procedure, mini-proinsulin reached nearly 0.3 g l-1 in the culture supernatant after 160 h of growth.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/B:BILE.0000044871.80632.f9</identifier><identifier>PMID: 15483385</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Ammonium ; Ammonium Sulfate - pharmacology ; Biological and medical sciences ; Bioreactors ; Bioreactors - microbiology ; Biotechnology ; Cell Culture Techniques - methods ; Cell Proliferation - drug effects ; Edetic Acid - pharmacology ; Fundamental and applied biological sciences. Psychology ; gene expression ; humans ; Hydrogen-Ion Concentration ; insulin ; Microbiology ; Nitrogen - metabolism ; pH effects ; Pichia - drug effects ; Pichia - genetics ; Pichia - growth & development ; Pichia - metabolism ; Pichia pastoris ; proinsulin ; Proinsulin - biosynthesis ; Proinsulin - genetics ; protein expression systems ; Q1 ; Q2 ; recombinant proteins ; Recombinant Proteins - biosynthesis ; Temperature ; Temperature effects</subject><ispartof>Biotechnology letters, 2004-08, Vol.26 (16), p.1269-1272</ispartof><rights>2004 INIST-CNRS</rights><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-6c2a2624f4de8f4b1720b2b4f154ee5896389ed6634bf1552f18b8ce9f59aedf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16198384$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15483385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pais-Chanfrau, J.M</creatorcontrib><creatorcontrib>Garcia, Y</creatorcontrib><creatorcontrib>Licor, L</creatorcontrib><creatorcontrib>Besada, V</creatorcontrib><creatorcontrib>Castellanos-Serra, L</creatorcontrib><creatorcontrib>Cabello, C.I</creatorcontrib><creatorcontrib>Hernandez, L</creatorcontrib><creatorcontrib>Mansur, M</creatorcontrib><creatorcontrib>Plana, L</creatorcontrib><creatorcontrib>Hidalgo, A</creatorcontrib><title>Improving the expression of mini-proinsulin in Pichia pastoris</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 °C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l-1 in the culture supernatant after 160 h of growth.</description><subject>Ammonium</subject><subject>Ammonium Sulfate - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Bioreactors - microbiology</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Proliferation - drug effects</subject><subject>Edetic Acid - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>insulin</subject><subject>Microbiology</subject><subject>Nitrogen - metabolism</subject><subject>pH effects</subject><subject>Pichia - drug effects</subject><subject>Pichia - genetics</subject><subject>Pichia - growth & development</subject><subject>Pichia - metabolism</subject><subject>Pichia pastoris</subject><subject>proinsulin</subject><subject>Proinsulin - biosynthesis</subject><subject>Proinsulin - genetics</subject><subject>protein expression systems</subject><subject>Q1</subject><subject>Q2</subject><subject>recombinant proteins</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Temperature</subject><subject>Temperature effects</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kVlLAzEUhYMoWqt_QQdBfZqafemDYMWlUFDQPofMNKmRWWrSEf33prZQ8MFwIXDzJTn3HADOEBwgiMnVaDgaT-4GcLUolQINJOQED5zaAT3EBMm5EHwX9CCiKGdU4QNwGON7wpWAYh8cIEYlIZL1wPW4XoT20zfzbPlmM_u1CDZG3zZZ67LaNz5Px76JXeWbLNWzL9-8yRYmLtvg4xHYc6aK9niz98H0_u719jGfPD2Mb28meUk5W-a8xAZzTB2dWelogQSGBS6oS0KsZVJxIpWdcU5okXoMOyQLWVrlmDJ25kgfXK7fTWo-OhuXuvaxtFVlGtt2UQvKEeYcq0Re_EtyrlIplsCzP-B724UmTaEFoVIykUztg-EaKkMbY7BOL4KvTfjWCOpVGHqkV2HobRj6NwztVlJONj90RW1n26sb9xNwvgFMLE3lgmlKH7ccR0oSSRN3uuacabWZJ9_19AVDRFKgTDEIyQ8nw5tJ</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Pais-Chanfrau, J.M</creator><creator>Garcia, Y</creator><creator>Licor, L</creator><creator>Besada, V</creator><creator>Castellanos-Serra, L</creator><creator>Cabello, C.I</creator><creator>Hernandez, L</creator><creator>Mansur, M</creator><creator>Plana, L</creator><creator>Hidalgo, A</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Improving the expression of mini-proinsulin in Pichia pastoris</title><author>Pais-Chanfrau, J.M ; Garcia, Y ; Licor, L ; Besada, V ; Castellanos-Serra, L ; Cabello, C.I ; Hernandez, L ; Mansur, M ; Plana, L ; Hidalgo, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-6c2a2624f4de8f4b1720b2b4f154ee5896389ed6634bf1552f18b8ce9f59aedf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Ammonium</topic><topic>Ammonium Sulfate - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Bioreactors - microbiology</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Proliferation - drug effects</topic><topic>Edetic Acid - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>insulin</topic><topic>Microbiology</topic><topic>Nitrogen - metabolism</topic><topic>pH effects</topic><topic>Pichia - drug effects</topic><topic>Pichia - genetics</topic><topic>Pichia - growth & development</topic><topic>Pichia - metabolism</topic><topic>Pichia pastoris</topic><topic>proinsulin</topic><topic>Proinsulin - biosynthesis</topic><topic>Proinsulin - genetics</topic><topic>protein expression systems</topic><topic>Q1</topic><topic>Q2</topic><topic>recombinant proteins</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Temperature</topic><topic>Temperature effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pais-Chanfrau, J.M</creatorcontrib><creatorcontrib>Garcia, Y</creatorcontrib><creatorcontrib>Licor, L</creatorcontrib><creatorcontrib>Besada, V</creatorcontrib><creatorcontrib>Castellanos-Serra, L</creatorcontrib><creatorcontrib>Cabello, C.I</creatorcontrib><creatorcontrib>Hernandez, L</creatorcontrib><creatorcontrib>Mansur, M</creatorcontrib><creatorcontrib>Plana, L</creatorcontrib><creatorcontrib>Hidalgo, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - 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Using this procedure, mini-proinsulin reached nearly 0.3 g l-1 in the culture supernatant after 160 h of growth.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>15483385</pmid><doi>10.1023/B:BILE.0000044871.80632.f9</doi><tpages>4</tpages></addata></record> |
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| ispartof | Biotechnology letters, 2004-08, Vol.26 (16), p.1269-1272 |
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| subjects | Ammonium Ammonium Sulfate - pharmacology Biological and medical sciences Bioreactors Bioreactors - microbiology Biotechnology Cell Culture Techniques - methods Cell Proliferation - drug effects Edetic Acid - pharmacology Fundamental and applied biological sciences. Psychology gene expression humans Hydrogen-Ion Concentration insulin Microbiology Nitrogen - metabolism pH effects Pichia - drug effects Pichia - genetics Pichia - growth & development Pichia - metabolism Pichia pastoris proinsulin Proinsulin - biosynthesis Proinsulin - genetics protein expression systems Q1 Q2 recombinant proteins Recombinant Proteins - biosynthesis Temperature Temperature effects |
| title | Improving the expression of mini-proinsulin in Pichia pastoris |
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