A method for designing peptide substrates for proteases. Tripeptidyl-p-nitroanilide substrates for subtilisin Carlsberg

1. The kinetic parameters of 25 peptidyl-p-nitroanilide substrates were investigated with subtilisin Carlsberg as model enzyme. 2. For a series of 12 substrates, the contribution of various side chains to the affinity constant was computed by regression analysis. From these contributions the sequenc...

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Veröffentlicht in:	European journal of biochemistry 1979-03, Vol.95 (1), p.115-119
Hauptverfasser:	Pozsgay, M, Gáspár, R, Bajusz, S, Elödi, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Kinetics Oligopeptides Substrate Specificity Subtilisins - metabolism
Online-Zugang:	Volltext
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