emPAI Calc—for the estimation of protein abundance from large-scale identification data by liquid chromatography-tandem mass spectrometry
emPAI Calc is an open-source web application for the estimation of protein abundance. It uses the correlation between the number of identified peptides and protein abundance in mass spectrometry-based proteomic experiments. The program is the first implementation of our previously reported emPAI alg...
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Veröffentlicht in: | Bioinformatics 2010-02, Vol.26 (4), p.576-577 |
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creator | Shinoda, Kosaku Tomita, Masaru Ishihama, Yasushi |
description | emPAI Calc is an open-source web application for the estimation of protein abundance. It uses the correlation between the number of identified peptides and protein abundance in mass spectrometry-based proteomic experiments. The program is the first implementation of our previously reported emPAI algorithm; it calculates the emPAI from the protein identification results obtained by database search engines such as Mascot.™ Availability: http://empai.iab.keio.ac.jp/; http://empai.iab.keio.ac.jp/supplement.php Source codes are available under Mozilla Public License. Contact: y-ishi@ttck.keio.ac.jp Supplementary Information: Supplementary data are available at Bioinformatics online. |
doi_str_mv | 10.1093/bioinformatics/btp700 |
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It uses the correlation between the number of identified peptides and protein abundance in mass spectrometry-based proteomic experiments. The program is the first implementation of our previously reported emPAI algorithm; it calculates the emPAI from the protein identification results obtained by database search engines such as Mascot.™ Availability: http://empai.iab.keio.ac.jp/; http://empai.iab.keio.ac.jp/supplement.php Source codes are available under Mozilla Public License. Contact: y-ishi@ttck.keio.ac.jp Supplementary Information: Supplementary data are available at Bioinformatics online.</description><subject>Algorithms</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>Databases, Protein</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Mathematics in biology. Statistical analysis. Models. Metrology. 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Data processing in biology (general aspects)</topic><topic>Proteins - chemistry</topic><topic>Proteomics - methods</topic><topic>Sequence Analysis, Protein</topic><topic>Software</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shinoda, Kosaku</creatorcontrib><creatorcontrib>Tomita, Masaru</creatorcontrib><creatorcontrib>Ishihama, Yasushi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioinformatics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Shinoda, Kosaku</au><au>Tomita, Masaru</au><au>Ishihama, Yasushi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>emPAI Calc—for the estimation of protein abundance from large-scale identification data by liquid chromatography-tandem mass spectrometry</atitle><jtitle>Bioinformatics</jtitle><addtitle>Bioinformatics</addtitle><date>2010-02-15</date><risdate>2010</risdate><volume>26</volume><issue>4</issue><spage>576</spage><epage>577</epage><pages>576-577</pages><issn>1367-4803</issn><eissn>1460-2059</eissn><eissn>1367-4811</eissn><abstract>emPAI Calc is an open-source web application for the estimation of protein abundance. 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subjects | Algorithms Biological and medical sciences Chromatography, Liquid - methods Databases, Protein Fundamental and applied biological sciences. Psychology General aspects Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects) Proteins - chemistry Proteomics - methods Sequence Analysis, Protein Software Tandem Mass Spectrometry - methods |
title | emPAI Calc—for the estimation of protein abundance from large-scale identification data by liquid chromatography-tandem mass spectrometry |
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