Prevalence of methicillin-resistant Staphylococcus aureus in meat
Recently the isolation of methicillin-resistant Staphylococcus aureus (MRSA) strains from several food-producing animals has been reported. During slaughtering of MRSA-positive animals, contamination of carcasses with MRSA may occur and consequently the meat of these animals may get contaminated. Th...
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| Veröffentlicht in: | International journal of food microbiology 2009-08, Vol.134 (1), p.52-56 |
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| creator | de Boer, E. Zwartkruis-Nahuis, J.T.M. Wit, B. Huijsdens, X.W. de Neeling, A.J. Bosch, T. van Oosterom, R.A.A. Vila, A. Heuvelink, A.E. |
| description | Recently the isolation of methicillin-resistant
Staphylococcus aureus (MRSA) strains from several food-producing animals has been reported. During slaughtering of MRSA-positive animals, contamination of carcasses with MRSA may occur and consequently the meat of these animals may get contaminated. The aim of this study was to estimate the prevalence of MRSA in raw meat samples from the retail trade.
Samples of raw beef, pork, veal, lamb/mutton, chicken, turkey, fowl and game were collected from the retail trade. A detection method including a two-step enrichment in Mueller–Hinton broth
+
6.5% NaCl and phenol red mannitol broth containing ceftizoxime and aztreonam, followed by isolation on MRSA ID agar (bioMérieux) was evaluated and subsequently applied for the detection of MRSA in samples of raw meats.
MRSA strains were isolated from 264 (11.9%) of 2217 samples analyzed. Isolation percentages for the meat species were: beef (10.6%), veal (15.2%), lamb and mutton (6.2%), pork (10.7%), chicken (16.0%), turkey (35.3%), fowl (3.4%) and game (2.2%). The majority (85%) of the isolated strains belonged to
spa-types of pulsed-field gel electrophoresis (PFGE) non-typeable (NT)-MRSA, corresponding to the multilocus sequence type ST398, a type also recently isolated in the Netherlands from pigs. However, a smaller part of these strains were found to be of other ST's, possibly of human origin.
Further studies are needed to elucidate transmission routes of MRSA in relation to meat and other foods and to provide the tools for preventing the spread of MRSA. At present the high prevalence of MRSA in meat has not been shown to contribute significantly to the dissemination of MRSA to humans and the possible health hazard for consumers of the presence of MRSA in foods should be further elucidated. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2008.12.007 |
| format | Article |
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Staphylococcus aureus (MRSA) strains from several food-producing animals has been reported. During slaughtering of MRSA-positive animals, contamination of carcasses with MRSA may occur and consequently the meat of these animals may get contaminated. The aim of this study was to estimate the prevalence of MRSA in raw meat samples from the retail trade.
Samples of raw beef, pork, veal, lamb/mutton, chicken, turkey, fowl and game were collected from the retail trade. A detection method including a two-step enrichment in Mueller–Hinton broth
+
6.5% NaCl and phenol red mannitol broth containing ceftizoxime and aztreonam, followed by isolation on MRSA ID agar (bioMérieux) was evaluated and subsequently applied for the detection of MRSA in samples of raw meats.
MRSA strains were isolated from 264 (11.9%) of 2217 samples analyzed. Isolation percentages for the meat species were: beef (10.6%), veal (15.2%), lamb and mutton (6.2%), pork (10.7%), chicken (16.0%), turkey (35.3%), fowl (3.4%) and game (2.2%). The majority (85%) of the isolated strains belonged to
spa-types of pulsed-field gel electrophoresis (PFGE) non-typeable (NT)-MRSA, corresponding to the multilocus sequence type ST398, a type also recently isolated in the Netherlands from pigs. However, a smaller part of these strains were found to be of other ST's, possibly of human origin.
Further studies are needed to elucidate transmission routes of MRSA in relation to meat and other foods and to provide the tools for preventing the spread of MRSA. At present the high prevalence of MRSA in meat has not been shown to contribute significantly to the dissemination of MRSA to humans and the possible health hazard for consumers of the presence of MRSA in foods should be further elucidated.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2008.12.007</identifier><identifier>PMID: 19144432</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Agar ; Animals ; antibiotic resistance ; bacterial contamination ; Bacterial Typing Techniques ; beef ; Biological and medical sciences ; Colony Count, Microbial ; Commerce ; Consumer Product Safety ; food contamination ; Food Contamination - analysis ; Food Contamination - prevention & control ; Food industries ; Food microbiology ; food retailing ; Fundamental and applied biological sciences. Psychology ; game meat ; Humans ; incidence ; lamb meat ; Meat ; Meat - microbiology ; Meat and meat product industries ; meat carcasses ; methicillin ; Methicillin-Resistant Staphylococcus aureus - classification ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; microbial contamination ; MLST ; molecular sequence data ; MRSA ; mutton ; nucleotide sequences ; pathogen identification ; pork ; poultry meat ; Prevalence ; public health ; spa-Typing ; Staphylococcus aureus ; strains ; turkey meat ; veal</subject><ispartof>International journal of food microbiology, 2009-08, Vol.134 (1), p.52-56</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-e800f4ba245cdd20d909cab883411cb737994bae87ee92d18004e7e07d8114223</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2008.12.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>310,311,315,781,785,790,791,3551,23932,23933,25142,27926,27927,45997</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22002312$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19144432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Boer, E.</creatorcontrib><creatorcontrib>Zwartkruis-Nahuis, J.T.M.</creatorcontrib><creatorcontrib>Wit, B.</creatorcontrib><creatorcontrib>Huijsdens, X.W.</creatorcontrib><creatorcontrib>de Neeling, A.J.</creatorcontrib><creatorcontrib>Bosch, T.</creatorcontrib><creatorcontrib>van Oosterom, R.A.A.</creatorcontrib><creatorcontrib>Vila, A.</creatorcontrib><creatorcontrib>Heuvelink, A.E.</creatorcontrib><title>Prevalence of methicillin-resistant Staphylococcus aureus in meat</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Recently the isolation of methicillin-resistant
Staphylococcus aureus (MRSA) strains from several food-producing animals has been reported. During slaughtering of MRSA-positive animals, contamination of carcasses with MRSA may occur and consequently the meat of these animals may get contaminated. The aim of this study was to estimate the prevalence of MRSA in raw meat samples from the retail trade.
Samples of raw beef, pork, veal, lamb/mutton, chicken, turkey, fowl and game were collected from the retail trade. A detection method including a two-step enrichment in Mueller–Hinton broth
+
6.5% NaCl and phenol red mannitol broth containing ceftizoxime and aztreonam, followed by isolation on MRSA ID agar (bioMérieux) was evaluated and subsequently applied for the detection of MRSA in samples of raw meats.
MRSA strains were isolated from 264 (11.9%) of 2217 samples analyzed. Isolation percentages for the meat species were: beef (10.6%), veal (15.2%), lamb and mutton (6.2%), pork (10.7%), chicken (16.0%), turkey (35.3%), fowl (3.4%) and game (2.2%). The majority (85%) of the isolated strains belonged to
spa-types of pulsed-field gel electrophoresis (PFGE) non-typeable (NT)-MRSA, corresponding to the multilocus sequence type ST398, a type also recently isolated in the Netherlands from pigs. However, a smaller part of these strains were found to be of other ST's, possibly of human origin.
Further studies are needed to elucidate transmission routes of MRSA in relation to meat and other foods and to provide the tools for preventing the spread of MRSA. At present the high prevalence of MRSA in meat has not been shown to contribute significantly to the dissemination of MRSA to humans and the possible health hazard for consumers of the presence of MRSA in foods should be further elucidated.</description><subject>Agar</subject><subject>Animals</subject><subject>antibiotic resistance</subject><subject>bacterial contamination</subject><subject>Bacterial Typing Techniques</subject><subject>beef</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>Commerce</subject><subject>Consumer Product Safety</subject><subject>food contamination</subject><subject>Food Contamination - analysis</subject><subject>Food Contamination - prevention & control</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>food retailing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>game meat</subject><subject>Humans</subject><subject>incidence</subject><subject>lamb meat</subject><subject>Meat</subject><subject>Meat - microbiology</subject><subject>Meat and meat product industries</subject><subject>meat carcasses</subject><subject>methicillin</subject><subject>Methicillin-Resistant Staphylococcus aureus - classification</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation & purification</subject><subject>microbial contamination</subject><subject>MLST</subject><subject>molecular sequence data</subject><subject>MRSA</subject><subject>mutton</subject><subject>nucleotide sequences</subject><subject>pathogen identification</subject><subject>pork</subject><subject>poultry meat</subject><subject>Prevalence</subject><subject>public health</subject><subject>spa-Typing</subject><subject>Staphylococcus aureus</subject><subject>strains</subject><subject>turkey meat</subject><subject>veal</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1vGyEQBmBUpWqctH8hdQ5VTrudAdawx8jqlxSpldKcEYbZBmt3cWDXUv59sWylOfY0B56ZgRfGrhFqBFx93tZh28Xoh-BSrDmArpHXAOoNW6BWbSXkCs7Yolhd4Qqac3aR8xYAGiHgHTvHFqWUgi_Y7a9Ee9vT6GgZu-VA02Nwoe_DWCXKIU92nJb3k909PvfRRefmvLRzolLCWLid3rO3ne0zfTjVS_bw9cvv9ffq7ue3H-vbu8o1XE0VaYBObiyXjfOeg2-hdXajtZCIbqOEattyTFoRtdxj4ZIUgfIaUXIuLtnNce4uxaeZ8mSGkB31vR0pztmo8malVaOKbI-ypJNzos7sUhhsejYI5hCg2ZpXAZpDgAa5KQGW3qvTlnkzkP_XeUqsgE8nYLOzfZfs6EJ-cbwM4wIP7uPRdTYa-ycV83DPAUXZrzhoWcT6KKiktg-UTHbh8BE-JHKT8TH8x4X_AkHSnaQ</recordid><startdate>20090831</startdate><enddate>20090831</enddate><creator>de Boer, E.</creator><creator>Zwartkruis-Nahuis, J.T.M.</creator><creator>Wit, B.</creator><creator>Huijsdens, X.W.</creator><creator>de Neeling, A.J.</creator><creator>Bosch, T.</creator><creator>van Oosterom, R.A.A.</creator><creator>Vila, A.</creator><creator>Heuvelink, A.E.</creator><general>Elsevier B.V</general><general>[Amsterdam; New York, NY]: Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090831</creationdate><title>Prevalence of methicillin-resistant Staphylococcus aureus in meat</title><author>de Boer, E. ; Zwartkruis-Nahuis, J.T.M. ; Wit, B. ; Huijsdens, X.W. ; de Neeling, A.J. ; Bosch, T. ; van Oosterom, R.A.A. ; Vila, A. ; Heuvelink, A.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-e800f4ba245cdd20d909cab883411cb737994bae87ee92d18004e7e07d8114223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Agar</topic><topic>Animals</topic><topic>antibiotic resistance</topic><topic>bacterial contamination</topic><topic>Bacterial Typing Techniques</topic><topic>beef</topic><topic>Biological and medical sciences</topic><topic>Colony Count, Microbial</topic><topic>Commerce</topic><topic>Consumer Product Safety</topic><topic>food contamination</topic><topic>Food Contamination - analysis</topic><topic>Food Contamination - prevention & control</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>food retailing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>game meat</topic><topic>Humans</topic><topic>incidence</topic><topic>lamb meat</topic><topic>Meat</topic><topic>Meat - microbiology</topic><topic>Meat and meat product industries</topic><topic>meat carcasses</topic><topic>methicillin</topic><topic>Methicillin-Resistant Staphylococcus aureus - classification</topic><topic>Methicillin-Resistant Staphylococcus aureus - isolation & purification</topic><topic>microbial contamination</topic><topic>MLST</topic><topic>molecular sequence data</topic><topic>MRSA</topic><topic>mutton</topic><topic>nucleotide sequences</topic><topic>pathogen identification</topic><topic>pork</topic><topic>poultry meat</topic><topic>Prevalence</topic><topic>public health</topic><topic>spa-Typing</topic><topic>Staphylococcus aureus</topic><topic>strains</topic><topic>turkey meat</topic><topic>veal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Boer, E.</creatorcontrib><creatorcontrib>Zwartkruis-Nahuis, J.T.M.</creatorcontrib><creatorcontrib>Wit, B.</creatorcontrib><creatorcontrib>Huijsdens, X.W.</creatorcontrib><creatorcontrib>de Neeling, A.J.</creatorcontrib><creatorcontrib>Bosch, T.</creatorcontrib><creatorcontrib>van Oosterom, R.A.A.</creatorcontrib><creatorcontrib>Vila, A.</creatorcontrib><creatorcontrib>Heuvelink, A.E.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Boer, E.</au><au>Zwartkruis-Nahuis, J.T.M.</au><au>Wit, B.</au><au>Huijsdens, X.W.</au><au>de Neeling, A.J.</au><au>Bosch, T.</au><au>van Oosterom, R.A.A.</au><au>Vila, A.</au><au>Heuvelink, A.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of methicillin-resistant Staphylococcus aureus in meat</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2009-08-31</date><risdate>2009</risdate><volume>134</volume><issue>1</issue><spage>52</spage><epage>56</epage><pages>52-56</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Recently the isolation of methicillin-resistant
Staphylococcus aureus (MRSA) strains from several food-producing animals has been reported. During slaughtering of MRSA-positive animals, contamination of carcasses with MRSA may occur and consequently the meat of these animals may get contaminated. The aim of this study was to estimate the prevalence of MRSA in raw meat samples from the retail trade.
Samples of raw beef, pork, veal, lamb/mutton, chicken, turkey, fowl and game were collected from the retail trade. A detection method including a two-step enrichment in Mueller–Hinton broth
+
6.5% NaCl and phenol red mannitol broth containing ceftizoxime and aztreonam, followed by isolation on MRSA ID agar (bioMérieux) was evaluated and subsequently applied for the detection of MRSA in samples of raw meats.
MRSA strains were isolated from 264 (11.9%) of 2217 samples analyzed. Isolation percentages for the meat species were: beef (10.6%), veal (15.2%), lamb and mutton (6.2%), pork (10.7%), chicken (16.0%), turkey (35.3%), fowl (3.4%) and game (2.2%). The majority (85%) of the isolated strains belonged to
spa-types of pulsed-field gel electrophoresis (PFGE) non-typeable (NT)-MRSA, corresponding to the multilocus sequence type ST398, a type also recently isolated in the Netherlands from pigs. However, a smaller part of these strains were found to be of other ST's, possibly of human origin.
Further studies are needed to elucidate transmission routes of MRSA in relation to meat and other foods and to provide the tools for preventing the spread of MRSA. At present the high prevalence of MRSA in meat has not been shown to contribute significantly to the dissemination of MRSA to humans and the possible health hazard for consumers of the presence of MRSA in foods should be further elucidated.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19144432</pmid><doi>10.1016/j.ijfoodmicro.2008.12.007</doi><tpages>5</tpages></addata></record> |
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| subjects | Agar Animals antibiotic resistance bacterial contamination Bacterial Typing Techniques beef Biological and medical sciences Colony Count, Microbial Commerce Consumer Product Safety food contamination Food Contamination - analysis Food Contamination - prevention & control Food industries Food microbiology food retailing Fundamental and applied biological sciences. Psychology game meat Humans incidence lamb meat Meat Meat - microbiology Meat and meat product industries meat carcasses methicillin Methicillin-Resistant Staphylococcus aureus - classification Methicillin-Resistant Staphylococcus aureus - isolation & purification microbial contamination MLST molecular sequence data MRSA mutton nucleotide sequences pathogen identification pork poultry meat Prevalence public health spa-Typing Staphylococcus aureus strains turkey meat veal |
| title | Prevalence of methicillin-resistant Staphylococcus aureus in meat |
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