Design of allele-specific primers and detection of the human ABO genotyping to avoid the pseudopositive problem

PCR experiments using DNA primers forming mismatch pairing with template lambda DNA at the 3' end were carried out in order to develop allele-specific primers capable of detecting SNP in genomes without generating pseudopositive amplification products, and thus avoiding the so-called pseudoposi...

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Veröffentlicht in:	Electrophoresis 2008-11, Vol.29 (20), p.4130-4140
Hauptverfasser:	Yaku, Hidenobu, Yukimasa, Tetsuo, Nakano, Shu-ichi, Sugimoto, Naoki, Oka, Hiroaki
Format:	Artikel
Sprache:	eng
Schlagworte:	3′ terminal mismatch pairings ABO Blood-Group System - genetics Allele-specific primer Alleles Base Pair Mismatch DNA Primers - genetics Human ABO gene Humans Polymerase Chain Reaction - methods Pseudopositive problem SNP
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