role of histone-like protein, Hlp, in Mycobacterium smegmatis dormancy
The role of histone-like protein (Hlp) in the development of a dormant state in long-incubated stationary-phase Mycobacterium smegmatis cells was studied in two models: (1) adoption of 'nonculturable' (NC) state, which is reversible due to resuscitation with proteinaceous resuscitation-pro...
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| Veröffentlicht in: | FEMS microbiology letters 2010-07, Vol.308 (2), p.101-107 |
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| creator | Anuchin, Aleksey M Goncharenko, Anna V Demina, Galina R Mulyukin, Andrey L Ostrovsky, Dmitry N Kaprelyants, Arseny S |
| description | The role of histone-like protein (Hlp) in the development of a dormant state in long-incubated stationary-phase Mycobacterium smegmatis cells was studied in two models: (1) adoption of 'nonculturable' (NC) state, which is reversible due to resuscitation with proteinaceous resuscitation-promoting factor (Rpf) and (2) the formation of morphologically distinct, ovoid resting forms. In the first model, inactivation of the hlp gene resulted in prolongation of culturability of starved cells followed by irreversible nonculturability when mycobacterial cells were unresponsive to resuscitation with Rpf. In the second model, M. smegmatis strain with the inactivated hlp gene was able to form dormant ovoid cells, but they were less resistant to heating and UV radiation than those of wild-type strain. The susceptibility of ovoid cells produced by Δhlp mutant to these damaging factors was probably due to a less condensed state of DNA, as revealed by fluorescent microscopy and DAPI staining. Evidently, Hlp is essential for cell viability at a later stage of NC dormancy or provides a greater stability of specialized dormant forms. |
| doi_str_mv | 10.1111/j.1574-6968.2010.01988.x |
| format | Article |
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In the first model, inactivation of the hlp gene resulted in prolongation of culturability of starved cells followed by irreversible nonculturability when mycobacterial cells were unresponsive to resuscitation with Rpf. In the second model, M. smegmatis strain with the inactivated hlp gene was able to form dormant ovoid cells, but they were less resistant to heating and UV radiation than those of wild-type strain. The susceptibility of ovoid cells produced by Δhlp mutant to these damaging factors was probably due to a less condensed state of DNA, as revealed by fluorescent microscopy and DAPI staining. Evidently, Hlp is essential for cell viability at a later stage of NC dormancy or provides a greater stability of specialized dormant forms.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2010.01988.x</identifier><identifier>PMID: 20497227</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Adoption ; Bacterial Proteins - physiology ; Cell viability ; Deactivation ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNA-Binding Proteins - physiology ; Dormancy ; Fluorescence ; Gene Deletion ; Gene Expression Regulation, Bacterial ; histone-like protein ; Histones ; Hot Temperature ; Humans ; Inactivation ; Microbial Viability - radiation effects ; Microbiology ; mycobacteria ; Mycobacterium smegmatis ; Mycobacterium smegmatis - genetics ; Mycobacterium smegmatis - growth & development ; Mycobacterium smegmatis - physiology ; Mycobacterium smegmatis - radiation effects ; nonculturability ; Prolongation ; Proteins ; Resuscitation ; Rpf ; Ultraviolet radiation ; Ultraviolet Rays</subject><ispartof>FEMS microbiology letters, 2010-07, Vol.308 (2), p.101-107</ispartof><rights>2010 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2010</rights><rights>2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2010 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4978-c7aeaf74b38b30fa27311dc621c3c5c3a85c6cad00bfec58b359c6cb54fe27b83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2010.01988.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2010.01988.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20497227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anuchin, Aleksey M</creatorcontrib><creatorcontrib>Goncharenko, Anna V</creatorcontrib><creatorcontrib>Demina, Galina R</creatorcontrib><creatorcontrib>Mulyukin, Andrey L</creatorcontrib><creatorcontrib>Ostrovsky, Dmitry N</creatorcontrib><creatorcontrib>Kaprelyants, Arseny S</creatorcontrib><title>role of histone-like protein, Hlp, in Mycobacterium smegmatis dormancy</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>The role of histone-like protein (Hlp) in the development of a dormant state in long-incubated stationary-phase Mycobacterium smegmatis cells was studied in two models: (1) adoption of 'nonculturable' (NC) state, which is reversible due to resuscitation with proteinaceous resuscitation-promoting factor (Rpf) and (2) the formation of morphologically distinct, ovoid resting forms. In the first model, inactivation of the hlp gene resulted in prolongation of culturability of starved cells followed by irreversible nonculturability when mycobacterial cells were unresponsive to resuscitation with Rpf. In the second model, M. smegmatis strain with the inactivated hlp gene was able to form dormant ovoid cells, but they were less resistant to heating and UV radiation than those of wild-type strain. The susceptibility of ovoid cells produced by Δhlp mutant to these damaging factors was probably due to a less condensed state of DNA, as revealed by fluorescent microscopy and DAPI staining. Evidently, Hlp is essential for cell viability at a later stage of NC dormancy or provides a greater stability of specialized dormant forms.</description><subject>Adoption</subject><subject>Bacterial Proteins - physiology</subject><subject>Cell viability</subject><subject>Deactivation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Dormancy</subject><subject>Fluorescence</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>histone-like protein</subject><subject>Histones</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Inactivation</subject><subject>Microbial Viability - radiation effects</subject><subject>Microbiology</subject><subject>mycobacteria</subject><subject>Mycobacterium smegmatis</subject><subject>Mycobacterium smegmatis - genetics</subject><subject>Mycobacterium smegmatis - growth & development</subject><subject>Mycobacterium smegmatis - physiology</subject><subject>Mycobacterium smegmatis - radiation effects</subject><subject>nonculturability</subject><subject>Prolongation</subject><subject>Proteins</subject><subject>Resuscitation</subject><subject>Rpf</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU9P2zAYh61piBa2rzAs7cClKa_tOLYPHBBa10lFHDbOluM6xV0Sd3Ei6LefQ4HDJoQvtt73efzvhxAmMCdpXGznhIs8K1Qh5xRSFYiScv74AU1fGx_RFJiQGQElJugkxi0A5BSKYzShkCtBqZiiRRdqh0OF733sQ-uy2v92eNeF3vl2hpf1boZ9i2_2NpTG9q7zQ4Nj4zaN6X3E69A1prX7T-ioMnV0n5_nU3S3-Pbrepmtbr__uL5aZTYdKDMrjDOVyEsmSwaVoYIRsrYFJZZZbpmR3BbWrAHKylmeIK5SoeR55agoJTtF54d90w3_DC72uvHRuro2rQtD1CIv0ndIpd4nGVNSkIIm8us_5DYMXZueoSmDguZcCUjUl2dqKBu31rvON6bb65evTMDlAXjwtdu_9gnoMTK91WMyekxGj5Hpp8j0o17crMZV8tnBD8PuDTv7z07W2cGqTNBm0_mo734mggGRnErB2V_h-6Dz</recordid><startdate>201007</startdate><enddate>201007</enddate><creator>Anuchin, Aleksey M</creator><creator>Goncharenko, Anna V</creator><creator>Demina, Galina R</creator><creator>Mulyukin, Andrey L</creator><creator>Ostrovsky, Dmitry N</creator><creator>Kaprelyants, Arseny S</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>201007</creationdate><title>role of histone-like protein, Hlp, in Mycobacterium smegmatis dormancy</title><author>Anuchin, Aleksey M ; Goncharenko, Anna V ; Demina, Galina R ; Mulyukin, Andrey L ; Ostrovsky, Dmitry N ; Kaprelyants, Arseny S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4978-c7aeaf74b38b30fa27311dc621c3c5c3a85c6cad00bfec58b359c6cb54fe27b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adoption</topic><topic>Bacterial Proteins - 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Academic</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anuchin, Aleksey M</au><au>Goncharenko, Anna V</au><au>Demina, Galina R</au><au>Mulyukin, Andrey L</au><au>Ostrovsky, Dmitry N</au><au>Kaprelyants, Arseny S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>role of histone-like protein, Hlp, in Mycobacterium smegmatis dormancy</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2010-07</date><risdate>2010</risdate><volume>308</volume><issue>2</issue><spage>101</spage><epage>107</epage><pages>101-107</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>The role of histone-like protein (Hlp) in the development of a dormant state in long-incubated stationary-phase Mycobacterium smegmatis cells was studied in two models: (1) adoption of 'nonculturable' (NC) state, which is reversible due to resuscitation with proteinaceous resuscitation-promoting factor (Rpf) and (2) the formation of morphologically distinct, ovoid resting forms. In the first model, inactivation of the hlp gene resulted in prolongation of culturability of starved cells followed by irreversible nonculturability when mycobacterial cells were unresponsive to resuscitation with Rpf. In the second model, M. smegmatis strain with the inactivated hlp gene was able to form dormant ovoid cells, but they were less resistant to heating and UV radiation than those of wild-type strain. The susceptibility of ovoid cells produced by Δhlp mutant to these damaging factors was probably due to a less condensed state of DNA, as revealed by fluorescent microscopy and DAPI staining. Evidently, Hlp is essential for cell viability at a later stage of NC dormancy or provides a greater stability of specialized dormant forms.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>20497227</pmid><doi>10.1111/j.1574-6968.2010.01988.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEMS microbiology letters, 2010-07, Vol.308 (2), p.101-107 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adoption Bacterial Proteins - physiology Cell viability Deactivation Deoxyribonucleic acid DNA DNA damage DNA-Binding Proteins - physiology Dormancy Fluorescence Gene Deletion Gene Expression Regulation, Bacterial histone-like protein Histones Hot Temperature Humans Inactivation Microbial Viability - radiation effects Microbiology mycobacteria Mycobacterium smegmatis Mycobacterium smegmatis - genetics Mycobacterium smegmatis - growth & development Mycobacterium smegmatis - physiology Mycobacterium smegmatis - radiation effects nonculturability Prolongation Proteins Resuscitation Rpf Ultraviolet radiation Ultraviolet Rays |
| title | role of histone-like protein, Hlp, in Mycobacterium smegmatis dormancy |
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