Infectious prion protein in the filtrate even after 15nm filtration
The evaluation of the removal efficacy during manufacturing is important for the risk assessment of plasma products with respect to possible contamination by infectious prions, as recently reported in several papers on the potential for prion transmission through plasma products. Here, we evaluated...
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| Veröffentlicht in: | Biologicals 2010-03, Vol.38 (2), p.311-313 |
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| container_title | Biologicals |
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| creator | Yunoki, Mikihiro Tanaka, Hiroyuki Urayama, Takeru Kanai, Yuta Nishida, Aya Yoshikawa, Maki Ohkubo, Yuji Kawabata, Yoshiyasu Hagiwara, Katsuro Ikuta, Kazuyoshi |
| description | The evaluation of the removal efficacy during manufacturing is important for the risk assessment of plasma products with respect to possible contamination by infectious prions, as recently reported in several papers on the potential for prion transmission through plasma products. Here, we evaluated a virus removal filter which has 15
nm pores. An antithrombin sample immediately prior to nano-filtration was spiked with prion material prepared in two different ways. The removal (log reduction factor) of prion infectivity using animal bioassays was ≥4.72 and 4.00 in two independent filtrations. However, infectivity was detected in both the pellet and supernatant following ultracentrifugation of the 15
nm filtered samples, indicating difficulty in complete removal. The data supports the conclusion that a certain amount of infectious prion protein is present as a smaller and/or soluble form (less than ∼15
nm in diameter). |
| doi_str_mv | 10.1016/j.biologicals.2009.10.018 |
| format | Article |
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nm pores. An antithrombin sample immediately prior to nano-filtration was spiked with prion material prepared in two different ways. The removal (log reduction factor) of prion infectivity using animal bioassays was ≥4.72 and 4.00 in two independent filtrations. However, infectivity was detected in both the pellet and supernatant following ultracentrifugation of the 15
nm filtered samples, indicating difficulty in complete removal. The data supports the conclusion that a certain amount of infectious prion protein is present as a smaller and/or soluble form (less than ∼15
nm in diameter).</description><subject>Infectivity</subject><subject>Prion</subject><subject>Removal</subject><subject>Soluble form</subject><subject>Virus removal filter</subject><issn>1045-1056</issn><issn>1095-8320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkE1LxDAQhoMouK7-h3ry1Dpp-pEcpfixsOBFz6FJJprSbTXJLvjvTVkFj0KYhJl5J-88hFxTKCjQ5nYolJvH-c3pfgxFCSBSvgDKT8iKgqhzzko4Xd5VnVOom3NyEcIAQGnVVivSbSaLOrp5H7IP7-YpxTmim7J04jtm1o3R9xEzPOCU9Taiz2g97X4LSXJJzmz6Ha9-7jV5fbh_6Z7y7fPjprvb5prVLOataS0i04wrIazqudVCtw0XzAI1XHBdQZlsGV2ZhoKhSvO-VaqpS1NxVbM1uTnOTRY_9xii3LmgcRz7CZN_2VYNAOPAU6c4dmo_h-DRyrTbrvdfkoJcuMlB_uEmF25LKXFL2u6oxbTKwaGXQTucNBrnEylpZvePKd-Xb30U</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Yunoki, Mikihiro</creator><creator>Tanaka, Hiroyuki</creator><creator>Urayama, Takeru</creator><creator>Kanai, Yuta</creator><creator>Nishida, Aya</creator><creator>Yoshikawa, Maki</creator><creator>Ohkubo, Yuji</creator><creator>Kawabata, Yoshiyasu</creator><creator>Hagiwara, Katsuro</creator><creator>Ikuta, Kazuyoshi</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20100301</creationdate><title>Infectious prion protein in the filtrate even after 15nm filtration</title><author>Yunoki, Mikihiro ; Tanaka, Hiroyuki ; Urayama, Takeru ; Kanai, Yuta ; Nishida, Aya ; Yoshikawa, Maki ; Ohkubo, Yuji ; Kawabata, Yoshiyasu ; Hagiwara, Katsuro ; Ikuta, Kazuyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-7d7fee3c38b99fba8fc9c76893f01d898c402114dc4d610d1bc8a7bb652d48b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Infectivity</topic><topic>Prion</topic><topic>Removal</topic><topic>Soluble form</topic><topic>Virus removal filter</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yunoki, Mikihiro</creatorcontrib><creatorcontrib>Tanaka, Hiroyuki</creatorcontrib><creatorcontrib>Urayama, Takeru</creatorcontrib><creatorcontrib>Kanai, Yuta</creatorcontrib><creatorcontrib>Nishida, Aya</creatorcontrib><creatorcontrib>Yoshikawa, Maki</creatorcontrib><creatorcontrib>Ohkubo, Yuji</creatorcontrib><creatorcontrib>Kawabata, Yoshiyasu</creatorcontrib><creatorcontrib>Hagiwara, Katsuro</creatorcontrib><creatorcontrib>Ikuta, Kazuyoshi</creatorcontrib><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Biologicals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yunoki, Mikihiro</au><au>Tanaka, Hiroyuki</au><au>Urayama, Takeru</au><au>Kanai, Yuta</au><au>Nishida, Aya</au><au>Yoshikawa, Maki</au><au>Ohkubo, Yuji</au><au>Kawabata, Yoshiyasu</au><au>Hagiwara, Katsuro</au><au>Ikuta, Kazuyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Infectious prion protein in the filtrate even after 15nm filtration</atitle><jtitle>Biologicals</jtitle><date>2010-03-01</date><risdate>2010</risdate><volume>38</volume><issue>2</issue><spage>311</spage><epage>313</epage><pages>311-313</pages><issn>1045-1056</issn><eissn>1095-8320</eissn><abstract>The evaluation of the removal efficacy during manufacturing is important for the risk assessment of plasma products with respect to possible contamination by infectious prions, as recently reported in several papers on the potential for prion transmission through plasma products. Here, we evaluated a virus removal filter which has 15
nm pores. An antithrombin sample immediately prior to nano-filtration was spiked with prion material prepared in two different ways. The removal (log reduction factor) of prion infectivity using animal bioassays was ≥4.72 and 4.00 in two independent filtrations. However, infectivity was detected in both the pellet and supernatant following ultracentrifugation of the 15
nm filtered samples, indicating difficulty in complete removal. The data supports the conclusion that a certain amount of infectious prion protein is present as a smaller and/or soluble form (less than ∼15
nm in diameter).</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.biologicals.2009.10.018</doi><tpages>3</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Infectivity Prion Removal Soluble form Virus removal filter |
| title | Infectious prion protein in the filtrate even after 15nm filtration |
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